pPIC9K序列

2023年12月4日发(作者：南京白鹭洲公园)

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载体基本信息

Invitrogen

pPIC9K, pPIC 9K

毕赤酵母蛋白表达载体

高拷贝

AOX1

多克隆位点，限制性内切酶

9276 bp

5´ AOX1:5´-GACTGGTTCCAATTGACAAGC-3´；

5' 测序引物及序列:

alpha-Factor-F: 5´-TACTATTGCCAGCATTGCTGC-3´

3' 测序引物及序列: 3´ AOX1:5´-GCAAATGGCATTCTGACATCC-3´

载体标签: N-alpha factor

载体抗性: 氨苄和卡那

筛选标记: HIS4

备注: 利用alpha分泌因子，分泌表达蛋白。

产品目录号: V175-20

稳定性: 稳定 Stable

组成型: 组成型 Constitutive

病毒/非病毒: 非病毒

出品公司:

载体名称:

质粒类型:

表达水平:

启动子:

克隆方法:

载体大小:

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载体质粒图谱和多克隆位点信息

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载体简介

pPIC9K载体中存在卡那抗性基因，允许利用卡那抗性在酵母体内筛选多克隆拷贝，载体其余部分与pPIC9载体完全一样。pPIC9K载体能够使用的毕赤酵母宿主菌是KM71和GS115。

pPIC9K载体详细信息如下：

• pPIC9载体大小9276 bp，是融合表达载体。

• 载体构建过程中，可供使用的单一限制性内切酶位点为SnaB I, EcoR I, Avr II, Not I.

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• 利用alpha因子分泌信号肽，分泌表达蛋白基因。

• 在载体构建过程中，你的基因必须保证与信号肽的起始密码子的读码框一致。

• 毕赤酵母中利用HIS4进行筛选。

• 为了将基因插入GS115或KM71的AOX区, 使用SacI限制性内切酶线性化质粒(GS115中产生His+Mut+基因型，KM71中产生His+ MutS基因型)

•为了将基因插入GS115或KM71的His4区, 使用Sal I或者Stu I限制性内切酶线性化质粒(GS115中产生His+ Mut+基因型，KM71中产生His+ MutS基因型)

•将基因插入GS115的AOX1区域，需要使用Bgl II线性化质粒 (产生His+MutS基因型)。

载体序列

LOCUS pPIC9K 9276 bp

DEFINITION pPIC9K

ACCESSION

KEYWORDS

SOURCE

ORGANISM other quences; artificial quences; vectors.

COMMENT This file is created by Vector NTI

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COMMENT VNTAUTHORNAME||

FEATURES Location/Qualifiers

source 1..9276

/organism="pPIC9K"

/mol_type="other DNA"

misc_feature 1152..1172

/label="alpha_factor_primer"

misc_feature complement(1327..1347)

DNA circular SYN

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/label="3_AOX1_primer"

CDS complement(1980..4514)

/label="ORF frame 2"

gene complement(4928..5743)

/label="KanR2"

/gene="KanR2"

CDS complement(4928..5743)

/label="ORF frame 3"

misc_feature complement(5908..5927)

/label="pBRrevBam_primer"

CDS complement(5909..6631)

/label="ORF frame 3"

misc_feature complement(6913..6935)

/label="pGEX_3_primer"

rep_origin complement(7332..7951)

/label="pBR322_origin"

gene complement(8106..8966)

/label="Ampicillin"

/gene="Ampicillin"

CDS complement(8106..8966)

/label="ORF frame 2"

promoter complement(9008..9036)

/label="AmpR_promoter"

ORIGIN

1 AGATCTAACA TCCAAAGACG AAAGGTTGAA TGAAACCTTT TTGCCATCCG ACATCCACAG

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61 GTCCATTCTC ACACATAAGT GCCAAACGCA ACAGGAGGGG ATACACTAGC AGCAGACCGT

121 TGCAAACGCA GGACCTCCAC TCCTCTTCTC CTCAACACCC ACTTTTGCCA TCGAAAAACC

181 AGCCCAGTTA TTGGGCTTGA TTGGAGCTCG CTCATTCCAA TTCCTTCTAT TAGGCTACTA

241 ACACCATGAC TTTATTAGCC TGTCTATCCT GGCCCCCCTG GCGAGGTTCA TGTTTGTTTA

301 TTTCCGAATG CAACAAGCTC CGCATTACAC CCGAACATCA CTCCAGATGA GGGCTTTCTG

361 AGTGTGGGGT CAAATAGTTT CATGTTCCCC AAATGGCCCA AAACTGACAG TTTAAACGCT

421 GTCTTGGAAC CTAATATGAC AAAAGCGTGA TCTCATCCAA GATGAACTAA GTTTGGTTCG

481 TTGAAATGCT AACGGCCAGT TGGTCAAAAA GAAACTTCCA AAAGTCGCCA TACCGTTTGT

541 CTTGTTTGGT ATTGATTGAC GAATGCTCAA AAATAATCTC ATTAATGCTT AGCGCAGTCT

601 CTCTATCGCT TCTGAACCCC GGTGCACCTG TGCCGAAACG CAAATGGGGA AACACCCGCT

661 TTTTGGATGA TTATGCATTG TCTCCACATT GTATGCTTCC AAGATTCTGG TGGGAATACT

721 GCTGATAGCC TAACGTTCAT GATCAAAATT TAACTGTTCT AACCCCTACT TGACAGCAAT

781 ATATAAACAG AAGGAAGCTG CCCTGTCTTA AACCTTTTTT TTTATCATCA TTATTAGCTT

841 ACTTTCATAA TTGCGACTGG TTCCAATTGA CAAGCTTTTG ATTTTAACGA CTTTTAACGA

901 CAACTTGAGA AGATCAAAAA ACAACTAATT ATTCGAAGGA TCCAAACGAT GAGATTTCCT

961 TCAATTTTTA CTGCAGTTTT ATTCGCAGCA TCCTCCGCAT TAGCTGCTCC AGTCAACACT

1021 ACAACAGAAG ATGAAACGGC ACAAATTCCG GCTGAAGCTG TCATCGGTTA CTCAGATTTA

1081 GAAGGGGATT TCGATGTTGC TGTTTTGCCA TTTTCCAACA GCACAAATAA CGGGTTATTG

1141 TTTATAAATA CTACTATTGC CAGCATTGCT GCTAAAGAAG AAGGGGTATC TCTCGAGAAA

1201 AGAGAGGCTG AAGCTTACGT AGAATTCCCT AGGGCGGCCG CGAATTAATT CGCCTTAGAC

1261 ATGACTGTTC CTCAGTTCAA GTTGGGCACT TACGAGAAGA CCGGTCTTGC TAGATTCTAA

1321 TCAAGAGGAT GTCAGAATGC CATTTGCCTG AGAGATGCAG GCTTCATTTT TGATACTTTT

1381 TTATTTGTAA CCTATATAGT ATAGGATTTT TTTTGTCATT TTGTTTCTTC TCGTACGAGC

1441 TTGCTCCTGA TCAGCCTATC TCGCAGCTGA TGAATATCTT GTGGTAGGGG TTTGGGAAAA

1501 TCATTCGAGT TTGATGTTTT TCTTGGTATT TCCCACTCCT CTTCAGAGTA CAGAAGATTA

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1561 AGTGAGAAGT TCGTTTGTGC AAGCTTATCG ATAAGCTTTA ATGCGGTAGT TTATCACAGT

1621 TAAATTGCTA ACGCAGTCAG GCACCGTGTA TGAAATCTAA CAATGCGCTC ATCGTCATCC

1681 TCGGCACCGT CACCCTGGAT GCTGTAGGCA TAGGCTTGGT TATGCCGGTA CTGCCGGGCC

1741 TCTTGCGGGA TATCGTCCAT TCCGACAGCA TCGCCAGTCA CTATGGCGTG CTGCTAGCGC

1801 TATATGCGTT GATGCAATTT CTATGCGCAC CCGTTCTCGG AGCACTGTCC GACCGCTTTG

1861 GCCGCCGCCC AGTCCTGCTC GCTTCGCTAC TTGGAGCCAC TATCGACTAC GCGATCATGG

1921 CGACCACACC CGTCCTGTGG ATCTATCGAA TCTAAATGTA AGTTAAAATC TCTAAATAAT

1981 TAAATAAGTC CCAGTTTCTC CATACGAACC TTAACAGCAT TGCGGTGAGC ATCTAGACCT

2041 TCAACAGCAG CCAGATCCAT CACTGCTTGG CCAATATGTT TCAGTCCCTC AGGAGTTACG

2101 TCTTGTGAAG TGATGAACTT CTGGAAGGTT GCAGTGTTAA CTCCGCTGTA TTGACGGGCA

2161 TATCCGTACG TTGGCAAAGT GTGGTTGGTA CCGGAGGAGT AATCTCCACA ACTCTCTGGA

2221 GAGTAGGCAC CAACAAACAC AGATCCAGCG TGTTGTACTT GATCAACATA AGAAGAAGCA

2281 TTCTCGATTT GCAGGATCAA GTGTTCAGGA GCGTACTGAT TGGACATTTC CAAAGCCTGC

2341 TCGTAGGTTG CAACCGATAG GGTTGTAGAG TGTGCAATAC ACTTGCGTAC AATTTCAACC

2401 CTTGGCAACT GCACAGCTTG GTTGTGAACA GCATCTTCAA TTCTGGCAAG CTCCTTGTCT

2461 GTCATATCGA CAGCCAACAG AATCACCTGG GAATCAATAC CATGTTCAGC TTGAGACAGA

2521 AGGTCTGAGG CAACGAAATC TGGATCAGCG TATTTATCAG CAATAACTAG AACTTCAGAA

2581 GGCCCAGCAG GCATGTCAAT ACTACACAGG GCTGATGTGT CATTTTGAAC CATCATCTTG

2641 GCAGCAGTAA CGAACTGGTT TCCTGGACCA AATATTTTGT CACACTTAGG AACAGTTTCT

2701 GTTCCGTAAG CCATAGCAGC TACTGCCTGG GCGCCTCCTG CTAGCACGAT ACACTTAGCA

2761 CCAACCTTGT GGGCAACGTA GATGACTTCT GGGGTAAGGG TACCATCCTT CTTAGGTGGA

2821 GATGCAAAAA CAATTTCTTT GCAACCAGCA ACTTTGGCAG GAACACCCAG CATCAGGGAA

2881 GTGGAAGGCA GAATTGCGGT TCCACCAGGA ATATAGAGGC CAACTTTCTC AATAGGTCTT

2941 GCAAAACGAG AGCAGACTAC ACCAGGGCAA GTCTCAACTT GCAACGTCTC CGTTAGTTGA

3001 GCTTCATGGA ATTTCCTGAC GTTATCTATA GAGAGATCAA TGGCTCTCTT AACGTTATCT

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3061 GGCAATTGCA TAAGTTCCTC TGGGAAAGGA GCTTCTAACA CAGGTGTCTT CAAAGCGACT

3121 CCATCAAACT TGGCAGTTAG TTCTAAAAGG GCTTTGTCAC CATTTTGACG AACATTGTCG

3181 ACAATTGGTT TGACTAATTC CATAATCTGT TCCGTTTTCT GGATAGGACG ACGAAGGGCA

3241 TCTTCAATTT CTTGTGAGGA GGCCTTAGAA ACGTCAATTT TGCACAATTC AATACGACCT

3301 TCAGAAGGGA CTTCTTTAGG TTTGGATTCT TCTTTAGGTT GTTCCTTGGT GTATCCTGGC

3361 TTGGCATCTC CTTTCCTTCT AGTGACCTTT AGGGACTTCA TATCCAGGTT TCTCTCCACC

3421 TCGTCCAACG TCACACCGTA CTTGGCACAT CTAACTAATG CAAAATAAAA TAAGTCAGCA

3481 CATTCCCAGG CTATATCTTC CTTGGATTTA GCTTCTGCAA GTTCATCAGC TTCCTCCCTA

3541 ATTTTAGCGT TCAACAAAAC TTCGTCGTCA AATAACCGTT TGGTATAAGA ACCTTCTGGA

3601 GCATTGCTCT TACGATCCCA CAAGGTGGCT TCCATGGCTC TAAGACCCTT TGATTGGCCA

3661 AAACAGGAAG TGCGTTCCAA GTGACAGAAA CCAACACCTG TTTGTTCAAC CACAAATTTC

3721 AAGCAGTCTC CATCACAATC CAATTCGATA CCCAGCAACT TTTGAGTTGC TCCAGATGTA

3781 GCACCTTTAT ACCACAAACC GTGACGACGA GATTGGTAGA CTCCAGTTTG TGTCCTTATA

3841 GCCTCCGGAA TAGACTTTTT GGACGAGTAC ACCAGGCCCA ACGAGTAATT AGAAGAGTCA

3901 GCCACCAAAG TAGTGAATAG ACCATCGGGG CGGTCAGTAG TCAAAGACGC CAACAAAATT

3961 TCACTGACAG GGAACTTTTT GACATCTTCA GAAAGTTCGT ATTCAGTAGT CAATTGCCGA

4021 GCATCAATAA TGGGGATTAT ACCAGAAGCA ACAGTGGAAG TCACATCTAC CAACTTTGCG

4081 GTCTCAGAAA AAGCATAAAC AGTTCTACTA CCGCCATTAG TGAAACTTTT CAAATCGCCC

4141 AGTGGAGAAG AAAAAGGCAC AGCGATACTA GCATTAGCGG GCAAGGATGC AACTTTATCA

4201 ACCAGGGTCC TATAGATAAC CCTAGCGCCT GGGATCATCC TTTGGACAAC TCTTTCTGCC

4261 AAATCTAGGT CCAAAATCAC TTCATTGATA CCATTATTGT ACAACTTGAG CAAGTTGTCG

4321 ATCAGCTCCT CAAATTGGTC CTCTGTAACG GATGACTCAA CTTGCACATT AACTTGAAGC

4381 TCAGTCGATT GAGTGAACTT GATCAGGTTG TGCAGCTGGT CAGCAGCATA GGGAAACACG

4441 GCTTTTCCTA CCAAACTCAA GGAATTATCA AACTCTGCAA CACTTGCGTA TGCAGGTAGC

4501 AAGGGAAATG TCATACTTGA AGTCGGACAG TGAGTGTAGT CTTGAGAAAT TCTGAAGCCG

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4561 TATTTTTATT ATCAGTGAGT CAGTCATCAG GAGATCCTCT ACGCCGGACG CATCGTGGCC

4621 GACCTGCAGG GGGGGGGGGG GCGCTGAGGT CTGCCTCGTG AAGAAGGTGT TGCTGACTCA

4681 TACCAGGCCT GAATCGCCCC ATCATCCAGC CAGAAAGTGA GGGAGCCACG GTTGATGAGA

4741 GCTTTGTTGT AGGTGGACCA GTTGGTGATT TTGAACTTTT GCTTTGCCAC GGAACGGTCT

4801 GCGTTGTCGG GAAGATGCGT GATCTGATCC TTCAACTCAG CAAAAGTTCG ATTTATTCAA

4861 CAAAGCCGCC GTCCCGTCAA GTCAGCGTAA TGCTCTGCCA GTGTTACAAC CAATTAACCA

4921 ATTCTGATTA GAAAAACTCA TCGAGCATCA AATGAAACTG CAATTTATTC ATATCAGGAT

4981 TATCAATACC ATATTTTTGA AAAAGCCGTT TCTGTAATGA AGGAGAAAAC TCACCGAGGC

5041 AGTTCCATAG GATGGCAAGA TCCTGGTATC GGTCTGCGAT TCCGACTCGT CCAACATCAA

5101 TACAACCTAT TAATTTCCCC TCGTCAAAAA TAAGGTTATC AAGTGAGAAA TCACCATGAG

5161 TGACGACTGA ATCCGGTGAG AATGGCAAAA GCTTATGCAT TTCTTTCCAG ACTTGTTCAA

5221 CAGGCCAGCC ATTACGCTCG TCATCAAAAT CACTCGCATC AACCAAACCG TTATTCATTC

5281 GTGATTGCGC CTGAGCGAGA CGAAATACGC GATCGCTGTT AAAAGGACAA TTACAAACAG

5341 GAATCGAATG CAACCGGCGC AGGAACACTG CCAGCGCATC AACAATATTT TCACCTGAAT

5401 CAGGATATTC TTCTAATACC TGGAATGCTG TTTTCCCGGG GATCGCAGTG GTGAGTAACC

5461 ATGCATCATC AGGAGTACGG ATAAAATGCT TGATGGTCGG AAGAGGCATA AATTCCGTCA

5521 GCCAGTTTAG TCTGACCATC TCATCTGTAA CATCATTGGC AACGCTACCT TTGCCATGTT

5581 TCAGAAACAA CTCTGGCGCA TCGGGCTTCC CATACAATCG ATAGATTGTC GCACCTGATT

5641 GCCCGACATT ATCGCGAGCC CATTTATACC CATATAAATC AGCATCCATG TTGGAATTTA

5701 ATCGCGGCCT CGAGCAAGAC GTTTCCCGTT GAATATGGCT CATAACACCC CTTGTATTAC

5761 TGTTTATGTA AGCAGACAGT TTTATTGTTC ATGATGATAT ATTTTTATCT TGTGCAATGT

5821 AACATCAGAG ATTTTGAGAC ACAACGTGGC TTTCCCCCCC CCCCCTGCAG GTCGGCATCA

5881 CCGGCGCCAC AGGTGCGGTT GCTGGCGCCT ATATCGCCGA CATCACCGAT GGGGAAGATC

5941 GGGCTCGCCA CTTCGGGCTC ATGAGCGCTT GTTTCGGCGT GGGTATGGTG GCAGGCCCCG

6001 TGGCCGGGGG ACTGTTGGGC GCCATCTCCT TGCATGCACC ATTCCTTGCG GCGGCGGTGC

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6061 TCAACGGCCT CAACCTACTA CTGGGCTGCT TCCTAATGCA GGAGTCGCAT AAGGGAGAGC

6121 GTCGAGTATC TATGATTGGA AGTATGGGAA TGGTGATACC CGCATTCTTC AGTGTCTTGA

6181 GGTCTCCTAT CAGATTATGC CCAACTAAAG CAACCGGAGG AGGAGATTTC ATGGTAAATT

6241 TCTCTGACTT TTGGTCATCA GTAGACTCGA ACTGTGAGAC TATCTCGGTT ATGACAGCAG

6301 AAATGTCCTT CTTGGAGACA GTAAATGAAG TCCCACCAAT AAAGAAATCC TTGTTATCAG

6361 GAACAAACTT CTTGTTTCGA ACTTTTTCGG TGCCTTGAAC TATAAAATGT AGAGTGGATA

6421 TGTCGGGTAG GAATGGAGCG GGCAAATGCT TACCTTCTGG ACCTTCAAGA GGTATGTAGG

6481 GTTTGTAGAT ACTGATGCCA ACTTCAGTGA CAACGTTGCT ATTTCGTTCA AACCATTCCG

6541 AATCCAGAGA AATCAAAGTT GTTTGTCTAC TATTGATCCA AGCCAGTGCG GTCTTGAAAC

6601 TGACAATAGT GTGCTCGTGT TTTGAGGTCA TCTTTGTATG AATAAATCTA GTCTTTGATC

6661 TAAATAATCT TGACGAGCCA AGGCGATAAA TACCCAAATC TAAAACTCTT TTAAAACGTT

6721 AAAAGGACAA GTATGTCTGC CTGTATTAAA CCCCAAATCA GCTCGTAGTC TGATCCTCAT

6781 CAACTTGAGG GGCACTATCT TGTTTTAGAG AAATTTGCGG AGATGCGATA TCGAGAAAAA

6841 GGTACGCTGA TTTTAAACGT GAAATTTATC TCAAGATCTC TGCCTCGCGC GTTTCGGTGA

6901 TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG GTCACAGCTT GTCTGTAAGC

6961 GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG

7021 CGCAGCCATG ACCCAGTCAC GTAGCGATAG CGGAGTGTAT ACTGGCTTAA CTATGCGGCA

7081 TCAGAGCAGA TTGTACTGAG AGTGCACCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA

7141 AGGAGAAAAT ACCGCATCAG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG

7201 GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA

7261 GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC

7321 CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC

7381 AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG

7441 TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC

7501 CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC AATGCTCACG CTGTAGGTAT

页脚内容10 1

7561 CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG

7621 CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC

7681 TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT

7741 GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT

7801 ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC

7861 AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA

7921 AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC

7981 GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC

8041 CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT

8101 GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA

8161 TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT

8221 GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA

8281 ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC

8341 ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG

8401 CGCAACGTTG TTGCCATTGC TGCAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT

8461 TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA

8521 AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA

8581 TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC

8641 TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG

8701 AGTTGCTCTT GCCCGGCGTC AACACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA

8761 GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG

8821 AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC

8881 ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG

8941 GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT

9001 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA

页脚内容11 1

9061 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG TCTAAGAAAC CATTATTATC

9121 ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTCTTCA AGAATTAATT

9181 CTCATGTTTG ACAGCTTATC ATCGATAAGC TGACTCATGT TGGTATTGTG AAATAGACGC

9241 AGATCGGGAA CACTGAAAAA TAACAGTTAT TATTCG

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