载体与引物表(完全)

2023年12月4日发(作者：什么是小数)

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TSINGKE武汉擎科新业生物技术有限公司载体和引物对应表Primer of VectorVectorpAAV-MCSPrimer(F)-CMV -FT7T3Primer(R)pFlag-CMV-R载体抗性-CMV --GAL4-2.1.R无反向引物42ADRBAC2PBAD30RP20M13F/T3pACT2pAD-GAL4-2.1Pao-aampAS2-1pB42ADPBACPAK8PBADPBAD30pBD-GAL4pBluescriptPbi121pBK-CMVpBR322pBR328pBridgepBS /M13-pBV220pCAMBIA 1301(1300)pCAMBIA 2300PCANTB5EpCAT3-enhancerpcDNA3.0pcDNA3.1pcDNA4pCE2.1pCEP4pCIpCI-neoPCMS-EGFPpCMV -HAPCMV-3Tag-4ApCMV5.0Pcmv5.-FlagpCMV-MYCpCMV-SportpCR2.1-TOPOPCR3.1pCS2PDBP17110(GAL4 AD)24990(GAL4 BD)pB42ADFBAC1TRXFPBAD30FP10T7/M13RAmp+Amp+35S-P. 3'T7pBR322.F无合适引物P24990(GAL4 BD)M13F/T7PBV220FP1M13R(-48)S1/M13RRVP3CMV-F/T7pcDNA3.1-F/pcDNA3.1F/T7T7/CMV-F(在T7前面）M13FpCEP-FpCI-F2/ T7(17ba)pCI-F2/ T7(19/17ba)T7 2GpCMV-FT3 /PFLAG-CMV-FPcmv5fPcmv5fpCMV-FT7M13F/31430T3/M13RPBV220RP2M13F(-47)S6BGH/SP6pcDNA3.1RBGHM13REBV-R(此载体没有反向引物)EBRT3T3pCMV-RT7Pcmv5rPcmv5rpCMV-RSP6//kanAmp+Amp+Amp+Amp+Amp+Amp+Amp+Amp+Amp+共3第1页TSINGKEPDC316武汉擎科新业生物技术有限公司VectorPrimer(F)Primer(R)pDonarM13FM13RpDONR207FORWARDREVERSEPdonr221M13FM13RPDONR221M13FM13RpDriveT7SP6pDsRED1-C1pDsRED-ex-C1-FpEGFP-N-3’(距离很近,一般不用)pDsRED2-C1pDsRED-ex-C1-FpEGFP-N-3’pDSRED-N1pEGFP-N1-5'pDSRED-N1-RPeasy-BLUNTM13RT7/M13FPeasy-blunt-simpleM13FM13R(克隆位点太近不建议用）Peasy-E1/E2T7T7TERPeasy-M1/M2CMV-FPeasy-T1M13RM13FPeasy-T1-simpleM13FM13R(克隆位点太近不建议用）Peasy-T3M13F/T7SP6/M13RpECFP-CpEGFP-C-5’pECFP-C-RpECFP-N1pEGFP-N-5’pEGFP-N-3’pEF/myc/ERpEF-FpCDNA3.1RpEGFP-1（BD BiosciencesClontech公司）pEGFP-CpEGFP-C-5’pEGFP-C-3'pEGFP-N(K+)pEGFP-N-5’pEGFP-N-3’pENTRM13FM13RpET-*(His)T7T7terpET32(a,b,c) (amp+)-N1pEGFP-N-5’pEGFP-N-3’-CMVpFLAG-CMV -FpFLAG-CMV -RpGAD424P171103'ADpGADGHP171103'ADpGADT7-RecP17110/T73'ADpGAPZa-Aa-FACTOR3'AOXpGBKT7(Kana+)T73'BDpGBT9pGBT9-FpGBT9-RpGEM-T(-Easy)M13F/T7SP6/M13RpGEX-(*)TpGEX-4T-5’pGEX-4T-3’PGL2GLP1GLP2PGL3RVP3GLP2(RVP4)PGM-TT7SP6PinpointTM VectorPinpoint PrimerSP6PIRESPIRES-F/T7T3/PIRES-RPrimer of Vector载体和引物对应表316.R载体抗性neo/kanneo/kanAmp+Amp+Amp+Amp+Amp+Amp+Amp+kan/neokan/neokan/neokan/neokankanAmp+kan/neokan +Amp+Amp+Primer of Vector共3第2页TSINGKE武汉擎科新业生物技术有限公司Primer(R)pIRES2-EGFP.P3’载体抗性Amp+载体和引物对应表VectorPrimer(F)pIRES2-EGFPpIRES2-EGFP.P5’PLEXAL44041pLXSNpLXSN-FpLXSNpLXSN-FPMPM-FpMAL-c2EMalE primerpMAL-C2xP5’pMAL-p2XMalE primerpMD18-TPQE30F/M13R(-48)/RV-MPmep4pMEP4-FpPIC9K(A+,Z+)5’AOX(pPIC9K-F)PPICZa5'AOX/PPICZa-FM13R(-48)6pRSETT7pSG5PSG5-FpShuttle-FpShuttle (stratagene公司）pShuttle-CMVCMV-FPsilencer2.1-u6 hygroT7pSilencer3.1-H1 hygroM13F(-47)01-TM13FpSK-CMVT7pSPORT1T7/M13RpSUPERT7pTA2M13F/T7(客户不要求情况下用pTAdv-TM13F /T7pTARGET TMpTarget.F(在T7前面）/T7pTRC99a-cpTRC99C-FpTrcHisAFpTRE2pTRE2-FPtriEX-1T7Ptriplex2T7pTWIN-1T7pTYB11NEB FpUC118M13R(-48)pUC18(19)M13F(-47)pUCm-TM13F/T7Pwsk29T7/M13F/M13F(-47)pXT7T7pYES2T7PZEOSV2T7M13F菌PCF-T(天为时代）T7天为时代TA载体反向无适合引物PM-RM13F(-47)P3’M13F(-47)M13F(-47)pMEP4-R3’AOX(pPIC9K-R)3'AOXpQE-R:无反向引物pSG5-RT7terpSG5-R无反向引物+Amp+Amp+Amp+Amp+Amp+Amp+Amp+M13RT3SP6/M13f(-47)T3T3/11751T7ter(客户确认再用）NEB RM13F(-47)M13R(-48)M13RT3/M13R/M13R(-48)13RT3

Amp+Amp+Amp+Amp+共3第3页

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