质粒测序引物

2023年12月4日发(作者：关于春节的传说)

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载体名称

P3*FLAG-CMV

pAc5.1/V5-His(_A,_B,_C)

pAcG2T

pACT

pACT2

pACT2

pACYC184(BamHI-site)

pACYCDuet-1(MCSI)

pACYCDuet-1(MCSII)

pAdEasy-1

pAdTrack-CMV

pAS2-1

pB42AD

pBABE

pBacPAK8

pBAD

pBAD/HisABC

pBBR1MCS

pBD-GAL4

pBI121

pBIND

pBK-CMV

pBluescriptIIKS(+/-)

pBluescriptIISK(+/-)

pBluescriptKS(+/-)

pBluescriptSK(+/-)

pBR322(BamHI-site)

pBR322(EcoRI/HindIII-sites)

pBR325(BamHI-site)

pBR329(BamHI-site)

pBridge(MCSI)

pBridge(MCSII)

pBS-T II

pBS（AMP）

pBSR

pBudCE4.1(MCSI)

pBudCE4.1(MCSII)

pBudCE4.1/lacZ/CAT

pBV220

pCAL-c

上游引物（5'primer）

CMV-30(825-854)

pAc5-5

pGEX-5

T7EEV

GAL4 ADfor

p17110

PBRforBam

ACYCDuetUP1 Primer

DuetUp2

not available

not available

GAL4 Bdfor/P24990

pB42ADF

pBMN_5'

BAC1

pBAD-F

pBAD-5'

T3/M13rev

pBD-GAL4-F

35S

GAL4-Bdfor

M13F/T7

T3/M13rev

T3/M13rev

T3/M13rev

T3/M13rev

PBRforBam

pBRforEco

PBRforBam

PBRforBam

GAL4-Bdfor

not available

T3/M13rev

T7

T3

CMV-Profor,T7

EF-1α Fwd

CMV-Profor,T7

pBV220F

T7/Tac-Profor

下游引物（3'primer）

CMV-24(1129-1152)

BGH rev

not available

T3/EBVrev

pGAL4-ADrv

p12584

PBRrevBam

DuetDOWN1

T7-Terminator

not available

not available

pGAL4-BDrv/P31430

PB42ADR

BAC2

pBAD-R

T7/M13for

PBD-GAL4-R

NOS

T3,EBVrev

M13R/T3

T7/M13for（优先用M13F）

T7/M13for

T7/M13for

T7/M13for

PBRrevBam

pBRrevHind

PBRrevBam

PBRrevBam

3'BD

not available

T7/M13for

T3

T7ter

c-mycrev,EBVrev

BGH

c-mycrev,EBVrev

PBV220R

T7-Terminator pCAL-n

pCAMBIA1301(1300)

pCAMBIA1304

pCAMBIA2300

pCANTB5E

pCAT3-enhancer

pCDFDuet-1(MCSI)

pCDFDuet-1(MCSII)

pcDNA1.1

pcDNA2.1

pcDNA3

pcDNA3.1(+/-)

pcDNA3.1/His(_A,_B,_C)

pcDNA3.1/myc-His(_A,_B,_C)

pcDNA3.1/V5-His(_A,_B,_C)

pcDNA3.1/Hygro(+,-)

pcDNA3.1/Zeo(+.-)

pcDNA3.3TOPOTA

pcDNA4/HisMax(_A,_B,_C)

pcDNA4/HisMax-TOPO

pcDNA4/HisMax-TOPO/lacZ

pcDNA4/myc-His(_A,_B,_C)

pcDNA4/TO

pcDNA4/V5-His(_A,_B,_C)

pcDNA4-TET/ON/AMP+

pcDNA5/FRT

pcDNA6/myc-His(_A,_B,_C)

pcDNA6/V5-His(_A,_B,_C)

pCEP4

pCI

pCI-neo

pCMS-EGFP

pCMS-EGFP

pCMV-3Tag-4A

pCMV5

pCMV6

pCMV6-XL4

pCMV-HA

pCMV-MYC(AMP+)

pCMVmyc/nuc

pcmv-scriptEXVector

pCMV-Sport6

pCOLADuet-1(MCSI)

T7/Tac-Profor

pCAMBIA1301-5'(ECORI)/M13R

pCAMBIA1301-5'(ECORI)

M13R(-48)

S1

RVP3

ACYCDuetUP1 Primer

DuetUP2

CMV-Profor,T7

M13for/T7

T7/CMV-Profor

pcDNA3.1F/T7/CMV-Profor

T7/CMV-Profor

T7/CMV-Profor

T7/CMV-Profor

T7/CMV-Profor

T7

CMV-Profor

Xpressfor

Xpressfor

Xpressfor

T7,CMV-Profor

TREfor/CMV-Profor

CMV-Profor,T7

CMV-Profor

T7/CMV-Profor

CMV-Profor,T7

CMV-Profor,T7

pCEP-F/CMV-Profor

T7（17BASE）

T7（17BASE）

T7

T7

T3 /PFLAG-CMV-F

pCMV5F

CMV-Profor

CMV-Profor/T7

PCMV-F,CMV-Profor,TREfor

PCMV-F

CMV-Profor

T3

T7/M13for

ACYCDuetUP1 Primer

T7-Terminator

pCAMBIA1301-3'(HindIII)

pCAMBIA1301-3'(HindIII)

M13F(-47)/M13F(-49)

S6

此载体无反向引物

DuetDOWN1

T7TER

pCDM8-3

M13rev/Tac-Profor

BGH rev/SP6

BGH rev

BGH rev

BGH rev/c-mycrev

BGH rev/V5rev

BGH rev/V5rev

BGHrev

TKPArever

BGH rev

BGH rev

BGH rev

BGH rev,c-mycrev

BGH rev

BGH rev,V5rev

BGH rev

BGH rev,c-mycrev

BGH rev,V5rev

EBVrev

EBVrev

EBVrev,T3

T3

EBVrev,T3

T7

PCMV5R

pCMV6-3、HGHrev

pCMV6-3、HGHrev

PCMV-R，EBVrev

PCMV-R, EBVrev

BGH rev,c-mycrev

T7

SP6/M13rev

DuetDOWN1 pCOLADuet-1(MCSII)

pCR2.1

pCR2.1-TOPO

pCR3.1

pCR3.1-Uni

pCR4

pCR4-Blunt

pCR4-TOPO

pCR-Blunt

pCR-BluntII

pCR-BluntII-TOPO

pCRII

pCRII-TOPO

pCR-ScriptSK(+)

pCR-XL-TOPO

pCS2+(MCSI)

pCS2+(MCSII)

pDB_GAL4cam

pDB_leu

pDEST22

pDEST15

pDEST20

pDEST26

pDisplay

pDNR-LIB(MCS_A)

pDonar

pDONR201

pDONR207

pDONR223

pDrive

pDsRED1-C1

pDsRed1-N1

pDsRed2-N1

pDsRed2-C1

pDsRED2-C1(KAN+)

pDsRed-Express

pDSRED-monomer-N1(KAN+)

pDSRED-N1(KAN+)

pEASY-Blunt

pEASY-BluntSimple

pEASY-T1 Simple

DuetUP2

T7,M13for

M13for,T7

T7

T7

T3/M13rev

T3/M13rev

M13rev,T3

M13rev

M13rev/SP6

M13rev/SP6

M13rev/SP6

M13rev/SP6

T7/M13for

M13rev

SP6

T3

T7P

PDBLeu-5

DEST22F

pGEX-5

pGEX-5

TREfor/CMV-Profor

T7/CMV-Profor

M13for,T7

M13F

PDONR-F

PDONR-F

M13F

T7/M13rev

(DsRed1-N-f)

CMV-Profor

(DsRed1-N-f)

(DsRed1-N-f)

pDsRED-ex-C1-F

dsRed1_N_primer/CMV-F

dsRed1_N_primer

pEGFP-N-5’

M13F

M13F

M13F

T7TER

M13rev

M13rev

BGH rev

BGH rev

T7/M13for

T7/M13for

M13for,T7

T7,M13for

T7/M13for

M13for/T7

T7/M13for

M13for/T7

T3/M13rev

T7,M13for

EBVrev,T7?

pSG5-3

PDBLeu-3

DEST22R

T7-Terminator

pFastBac-3

T7/M13for

c-mycrev

pSG5-3,M13R

M13R

PDONR-R

PDONR-R

M13R

SP6/M13for

DsRed1-C-r

RFP-Nrev,CMV-R?

DsRed1-C-r

DsRed1-C-r

pEGFP-N-3’

dsRed1_C_primer/CMV-R

pDSRED-N-R

T7/ M13R

T7/ M13R（距离插入位点合适，优先安排）

M13R（距离插入位点合适，优先安排） pEASY-T1

pEASY-T3

pECFP-N(C)

pEF/myc/cyto/ER/mito/nuc

pEF1(4,6/myc-His)

pEF1(4,6/V5-His)

pEF1(4;6/His_A;_B;_C)

pEF5/FRT/V5-D(-TOPO)

pEGFP

pEGFP-1

pEGFP-C

pEGFP-C1

pEGFP-C2

pEGFP-C3

pEGFP-F

pEGFP-N

pEGFP-N1

pEGFP-N2

pEGFP-N3

pENTR

pET-11(-a,-b,-c,-d)

pET-12(-a,-b,-c)

pET-14b

pET-15b

pET-16b

pET-17b

pET-17xb

pET-19b

pET-20b(+)

pET-21(-a,-b,-c,-d)(+)

pET-22b(+)

pET-23(-a,-b,-c,-d)(+)

pET-24(-a,-b,-c,-d)(+)

pET-25b(+)

pET-26b(+)

pET-27b(+)

pET-28a(-b,-c)(+)

pET-29a(-b,-c)(+)

pET-3(-a,-b,-c,-d)

pET-30_Ek/LIC

pET-30_Xa/LIC

pET-30a(-b,-c)(+)

pET-31b(+)

M13F/T7

M13F/T7

PECFP-N-5.

pEF-5

T7/pEF-5

T7/pEF-5

T7/Xpressfor

T7/pEF-5

M13rev

not available

PEGFP-C-5′

EGFP-Cfor

EGFP-Cfor

EGFP-Cfor

EGFP-Cfor

PEGFP-N-5′

CMV-Profor

CMV-Profor

CMV-Profor

M13F

T7

T7

T7

T7

T7

T7

T7

T7

T7

T7

T7

T7

T7

T7

T7

T7

T7

/T7

T7

T7

T7

/T7

not available

M13R

M13R/SP6

PECFP-N-3,

Pcdna3.1R/BGH rev

Pcdna3.1R/BGH rev

Pcdna3.1R/BGH rev

Pcdna3.1R/BGH rev

Pcdna3.1R/BGH rev

EGFP-Nrev

EGFP-Nrev/ PEGFP-N-3′

PEGFP-C-3′

SV40-pArev

SV40-pArev

SV40-pArev

SV40-pArev

PEGFP-N-3′

EGFP-Nrev

EGFP-Nrev

EGFP-Nrev

M13R

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator pET-32_Ek/LIC

pET-32_XA/LIC

pET-32a(-b,-c)(+)

pET-33b(+)

pET-34b(+)

pET-35b(+)

pET-36b(+)

pET-37b(+)

pET-38b(+)

pET-39b(+)

pET-40b(+)

pET-41_Ek/LIC

pET-41a(-b,-c)(+)

pET-42a(-b,-c)(+)

pET-43.1a(-b,-c)(+)

pET-43.1a_EK/LIC

pET-44a(-b,-c)(+)

pET-44a_EK/LIC

pET-45b(+)

pET-46_Ek/LIC

pET-5(-a,-b,-c)

pET-52b(+)

pET-7

pET-9(-a,-b,-c,-d)

pETcoco-1

pETDuet-1(MCSI)

pETDuet-1(MCSII)

pET-T4

pEYFP-C1

pEYFP-N1

pFLAG-ATS

pFLAG-CTC

pFLAG1

pFLAG-CMV(-2)

pFLAG-CMV1

pGAD424

pGADGH

pGADT7

pGADT7-Rec

pGADT-T

pGAD10

pGAPZa-A

pGBKT7

TRXfor

TRXfor

/TRXfor

T7

not available

GST-Cfor

/pGEX-5

/pGEX-5

T7

T7

T7

T7

T7

T7

T7

pETUP1

DuetUp2

T7

pEGFP-C-5’

pEGFP-N-5’,CMV-Profor

N26for

N26for

N26for

CMV-30；CMV-Profor

CMV-30

GAL4_ADfor

GAL4_ADfor

T7/GAL4_ADfor

T7/GAL4_ADfor

T7

GAL4_ADfor

a-FACTOR

T7

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

coliDOWN

not available

T7-Terminator

not available

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

T7-Terminator

DuetDOWN1

T7-Terminator

T7-Terminator

pEGFP-C-3'

pEGFP-N-3’

pTrcHis-rv；C24rev

pTrcHis-rv；C24rev

pTrcHis-rv；C24rev

CMV-24

CMV-24

3'AD

3'AD

3'AD

3'AD

3,BD

3'AD

3'AOX

3,BD pGBT9

pGEM-1

pGEM-3

pGEM-3Zf(+)(-)

pGEM-4

pGEM-4Z

pGEM-5Zf(+)(-)

pGEM-7Zf(+)(-)

pGEM-9Zf(+)(-)

pGEM-T

pGEM-T_Easy

pGene/V5-His_A(_B,_C)

pGEX-2T

pGEX-2TK

pGEX-3X

pGEX-4T-1(-2,-3)

pGEX-5X-1(-2,-3)

pGEX-6P-1(-2,-3)

pGL2

pGL3

pGL3-Basic(MCSI)

pGL3-Basic(MCSII)

pGL4

pGlow(-TOPO)

pGM-T

pGWC

pHook-2

pIND/V5-His_A(_B,_C)

PinpointTMVector

pIRES(MCSI)

pIRES(MCSII)

pIRES2-EGFP

pIRES-hrGFP-2a

pIRESneo2

pIVEX(all)

pLEXA

pLNCX

pLNCX2

pLVTHM（H1启动子下游）

pLXSN

pMALc

MATCHMAKER5'DNA-BD/GAL4BD

T7

T7

SP6/M13rev

T7

T7/M13for

SP6/M13rev

SP6/M13rev

T7/M13for

T7/M13for

T7/M13for

pGENE-5

pGEX-5

pGEX-5

pGEX-5

pGEX-5

pGEX-5

pGEX-5

GLP1

RVP3

RVP3(PGL3+)?

RVP4,

T7

T7

SP6

T7/CMV-Profor

not available

PinpointPrimer

T7

not available

CMV-Profor,pIRES2-EGFP.P5’

CMV-Profor,T3

T7/CMV-Profor

T7/pBRrevBam

pLEXA-F

pLNCX-F

CMV-Profor

H1primer

pLXSN-F

MalE primer

MATCHMAKER3'DNA-BD

SP6

SP6

T7/M13for

SP6

SP6/M13rev

T7/M13for

T7/M13for

SP6/M13rev

SP6/M13rev

SP6/M13rev

BGH rev,V5rev

pGEX-3

pGEX-3

pGEX-3

pGEX-3

pGEX-3

pGEX-3

GLP2

GLP2(RVP4)(没有特别说明用GLP2)

GLP2(PGL3-)?

PLXSN-3'?

pGlow-3

SP6

T7

not available

BGH rev,V5rev

SP6

pIRES-3

T3,EBVrev

IRESrev,pIRES2-EGFP.P3’

pIRES-hrGFP-3

pIRES-3

T7-Terminator

pLEXA-R

pLNCX-R

pRevTRE-3

SP6

pLXSN-R

M13F(-47) pMAL-c2E

pMAL-C2x

pMALd

pMALe

pMAL-P4X

pMAL-p2X

pMET A/B/C

pMD18-T

pMD19-T

pMD20-T

pMOSBlue

pMSCV-puro

pMT/V5-His_A(_B,_C)

pNTAP

pCTAP

pOTB7

pOptiVEC-TOPO TA

pPC86

pPC97

pPIC3.5K

pPic9k(A+,Z+)

pPICZa

pPROEXHTA

pProEX-HTa(b,c)

pPD129.36

pQE30or40or60(AMP+)

pRC/CMV2/CAT

pRECEIVER

pRECEIVER-M03

pREP4

pRK5

pRSET

pRSFDuet-1(MCSI)

pRSFDuet-1(MCSII)

pSecTac2

pSG5

pSHlox1

pSHUTTLE-CMV

pSI

pSilencer1.0-U6

pSilencer2.0-U6

pSilencer2.1-U6neo

pSilencer3.1-H1hygro

MalE primer

Pmal-c2x-F(P5’)

MalE primer

MalE primer

MalE primer

MalE primer

AUG1F

M13R(-48)ECORI

M13F47

M13F47

T7/M13rev

pLXSNfw

Mtfor

T3

pCTAP5'primer

M13rev/T7

CMV-F/CMV-Profor

PPC86-F

PBD-GAL4-F

5'AOX

5’AOX(Ppic9k-f)/a-factor

5'AOX/PPICZa-F

M13R(-48)

M13R48/ Tac-Profor

PQE30-F

CMV/T7

CMV-F 此载体

pRECEIVER-M03F

pREP4 forward primer

SP6

T7/Xpressfor

DuetUP1

DuetUP2

T7/CMV-Profor

pSG5-5,/T7

T7

PSHUTTLE-CMV-F

T7

T7

T7

T7

M13F(-47)

M13F(-47)

Pmal-C2X-R(P3’ )

M13F(-47)

M13F(-47)

M13F(-47)

M13F(-47)

AUG1R

M13F(-47)(HINDⅢ)

M13R48

M13R48

M13for

pMSCVrev

BGH rev,V5rev

T7

T7

M13for/SP6

EMCVIRES rever

PPC86-R

PBD-GAL4-R

3'AOX

3’AOX(Ppic9k-R)

3'AOX

无反向引物

pBADrv/pTrcHis-3

M13F20/M13F

PQE-30R

无反向引物

pRECEIVER-M03R

EBV rever primer

pSG5-3

T7ter

DuetDOWN1

T7TER

BGH rev/c-mycrev

PSG5-R(SV40)

SP6

PSHUTTLE-CMV-R

EBVrev

T3

M13for

M13for

3.0REV pSilencer4_1-CMVneo

pSILENCEV2.0-U6

pSIneo

Psit

pSK01-T

pSK-CMV(KAN+)

pSOS

pSP64

pSP64/65

pSP65

pSP70

pSP71

pSP72

pSP73

pSport1

pSPORT1

pSPT18

pSTBlue

pSUPER

pSURE-T

pSURE-Tsimple

pSuperCos

pT3T7

pT7-7

pT7Blue®

pT7T3_18U

pT7T3D-PAC

pTA2

pT-Adv

pTAg

pTARGETTM

pTG19-T

pTARGETTM

pThioHisA,B,andC

pTO-T7

pTRC99A

pTrc99a-c

pTRC99a-c

pTrcHisB

pTrcHisA

pT-REX-DEST30

pTriEx1

pSilener4.1-5'

T7

T7

T7

M13F

T7

Psos5'

SP6

SP6

SP6

SP6

SP6

SP6

SP6

SP6/M13F(-47)

SP6/M13for

SP6

T7/M13rev

T7/M13F

T7

M13F

M13F-47

pBRforEco

M13rev/T3

T7

T7

M13rev/T7

M13rev/T7

M13F/T7(优先使用M13F）

M13rev

T7/M13rev

pTarget.F(在T7前面）/T7

M13F(-47)

T7/M13F

Trx Forward

T7

PTRC99A-F

PTRC99C-F

PTRC99C-F

PTrcHisA-F/Xpressfor

PTrcHisA-F

TREfor

T7/TriExup

CMV-Profor

2.0REV

EBVrev,T3

T7T

M13R

T3

Psos3'

M13rev

此载体无反向引物

M13rev

T7

T7

T7

T7

T7/M13R

T7/M13rev

T7

SP6/M13for

M13R

T3

M13R

M13R-48

T7

M13for/T7

not available

M13F(-47)

M13for/T3

M13for/T3

T3/M13R

T7,M13for

M13for

pTarget.R

M13R(-48)

PTARGET-SEQ

Trx Rever / M13for

T7TER

pTrcHis-rv

PTRC99C-R

PTRC99C-R

PTrcHisA-R

PTrcHisA-R

T7

TriExdown pTriEx1.1

pTriEx1.1_Hygro

pTriEx1.1_Neo

pTriplEX1

pTriplEX2

pTWIN-1

pTXB1

pTXB2

pTXB3

pTYB1

pTYB2

pTYB3

pTYB4

pTZ_18U

pUB6/V5-His

pUC13

pUC18

pUC18(19)/118(119)

pUC19

pUC57

pUC8

pUC9

pUM-T

pUCm-T(AMP+)

pUNI/V5-His

pVAX1T7

pVP22/mycHis

pVP22/mycHis2

pWE15

pYES2

pYES-DEST52

pYEX_4T_1

pYEX_4T_2

pYEX_4T_3

pYEX_4T_4

pZeoSV2+/hu-Tyr

pZERO-1

pZERO-2

pZome-1-C

pZome-1-N

YCp50(BamHI-site)

YEp13(BamHI-site)

YEp24(BamHI-site)

T7/TriExup

T7/TriExup

T7/TriExup

TriplEx-LD-5

TriplEx-LD-5；pTR5‘

T7

T7

T7

T7

T7

T7

T7

T7

T7/M13rev

UB-F

M13rev/M13rev(-48)

M13rev/M13rev(-48)

M13F(-47)

M13rev/M13rev(-48)

M13rev/M13rev(-48)

M13rev/M13rev(-48)

M13rev/M13rev(-48)

M13F(-20)

M13F/T7

not available

T7/CMV-Profor

VP22-Cfor

T7/CMV-Profor

pBRrevHind

T7

T7/GAL1-Profor

Clontech

pGEX-5

pGEX-5

pGEX-5

T7

M13rev/SP6

M13rev/SP6

Zome-F

Zome-F

PBRforBam

PBRforBam

PBRforBam

TriExdown

not available

IRESrev

T7,TriplEx-LD-3

T7,TriplEx-LD-3

T7TER

MxeInteinrev

MxeInteinrev

MxeInteinrev

Inteinrev

Inteinrev

Inteinrev

Inteinrev

M13for

BGH rev,V5rev

M13for/M13for(-49)

M13for/M13for(-49)

M13R(-48)/PUC19(HINDⅢ)

M13for/M13for(-49)

M13for/M13for(-49)

M13for/M13for(-49)

M13for/M13for(-49)

M13R(-20)

M13R

BGH rev,V5rev

PCDNA3.1R/BGHR

BGH rev,c-mycrev

VP22-Nrev

T7

pYes2.R

V5rev/CYC1-Terminator

pYEX_4T-3

pYEX_4T-4

pYEX_4T-4

pYEX_4T-4

not available

T7/M13for

M13for/T7

Zome-R

Zome-R

PBRrevBam

PBRrevBam

PBRrevBam YIp5(BamHI-site)

RNAi-ReadypSIREN-RetroQ-U6ZsGreen

菌PCDB

天为时代TA载体

m13（+）

pMSCV-neo

PBRforBam

U6

T7

T7

T7？

pMSCV-5'

PBRrevBam

BGH

T3

T3？

pMSCV-3'

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