Hybrid QconCAT-Bad Targeted Absolute and Data-Independent Acquisition-Bad Label-Free Quantification Enables In-Depth Proteomic
Characterization of Wheat Amyla/Trypsin
Inhibitor Extracts
期刊名称： Journal of Proteome Rearch
年份： 2021年
期号： 第3期
关键词： non-celiac wheat nsitivity;celiac dia;alpha-amyla/trypsin
inhibitor;flour;Triticum aestivum;wheat;label-free quantification;QconCAT;data-independent acquisition;bottom-up proteomics
摘要：Wheatamyla/trypsininhibitors (ATIs) have gained significant relevance as inducers ofintestin
al and extra-intestinal inflammation. In this study, we prenta novel hybrid data-independent acquisition (DIA) liquid chromatography–massspectrometry (LC-MS) approach, combining QconCAT technology with shortmicroflow LC gradients and DIA and apply the method toward the quantitativeproteome analysis of ATI extracts. The prented method is fast, robust,and reproducible and provides preci QconCAT-bad absolute quantificationof major ATI proteins while simultaneously quantifying the proteomeby label-free quantification (LFQ). We analyzed extracts of 60 varietiesof common wheat grown in replication and evaluated the reproducibilityand precision of the workflow for the quantification of ATIs. Applyingthe method to analyze
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