Development and Validation of a HPLC Method for the Determination of Five Bioactive Compounds in
the "Xuebijing" Injection
期刊名称: Analytical Letters
作者: Chen, Yong,Li, Yerui,Chen, Xueying,Wang, Longhu,Sun, Changhai,Yan,
Wenjie,Liu, Xuesong
年份: 2010年
期号: 第15期
关键词: Quantitative analysis; RP-HPLC; TCM; Timed wavelength measure;
oXuebijingo injection
摘要:A reverd pha high performance liquid chromatographic (RP-HPLC) method with ultraviolet (
UV) detector was developed for simultaneously determining five bioactive components (i.e., hydroxysafflor yellow A: HSYA; paeoniflorin, ferulic acid, benzoic acid, and danshensu) in "Xuebijing" (XBJ) injection, a widely ud traditional Chine medicine (TCM) for treating psis and multiple organ dysfunction syndrome. A Zorbox SB C18 column was ud with 0.2% phosphoric acid (V/V)-acetonitrile as the mobile pha under the condition of gradient elution. The five components were analyzed by using a timed wavelength measure according to their maximum absorption wavelength. The intraday and interday precisions of the five investigated compounds were less than 1.17% and
the average recoveries ranged from 97.3% to 103.2%. There were
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