Development and Validation of a HPLC Method for the Determination of Five Bioactive Compounds in
the "Xuebijing" Injection
期刊名称： Analytical Letters
作者： Chen, Yong,Li, Yerui,Chen, Xueying,Wang, Longhu,Sun, Changhai,Yan,
Wenjie,Liu, Xuesong
年份： 2010年
期号： 第15期
关键词： Quantitative analysis; RP-HPLC; TCM; Timed wavelength measure;
oXuebijingo injection
摘要：A reverd pha high performance liquid chromatographic (RP-HPLC) method with ultraviolet (
UV) detector was developed for simultaneously determining five bioactive components (i.e., hydroxysafflor yellow A: HSYA; paeoniflorin, ferulic acid, benzoic acid, and danshensu) in "Xuebijing" (XBJ) injection, a widely ud traditional Chine medicine (TCM) for treating psis and multiple organ dysfunction syndrome. A Zorbox SB C18 column was ud with 0.2% phosphoric acid (V/V)-acetonitrile as the mobile pha under the condition of gradient elution. The five components were analyzed by using a timed wavelength measure according to their maximum absorption wavelength. The intraday and interday precisions of the five investigated compounds were less than 1.17% and
the average recoveries ranged from 97.3% to 103.2%. There were
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