专利名称：METHOD FOR THE INDIVIDUAL
ADAPTATION OF EXCITATION INTENSITIES
IN A MULTIBAND FLUORESCENCE
MICROSCOPE AND MULTIBAND
FLUORESCENCE MICROSCOPE FOR
CARRYING OUT SAID METHOD
发明人：WEISS, ALBRECHT
申请号：EP99962089.1
申请日：19991127
公开号：EP1141764A1
公开日：
20011010
专利内容由知识产权出版社提供
摘要：The invention relates to a method for the individual adaptation of excitation intensities in a multiband fluorescence microscope with veral excitation bands that are different in their spectrums and with pertaining fluorescence bands. The intensities of the individual fluorescence bands in the microscope image are determined and compared with standard intensity values greater than or equal to zero. For every excitation band that is assigned to a fluorescence intensity deviating from the standard intensity values a lective filter (23; 28, 29, 31, 32) is introduced into the illumination optical path. The transmission degree of said illumination optical path is variably adjusted in such a
manner that by attenuating the excitation band the pertaining fluorescence intensity is adjusted to its standard intensity value. A multiband fluorescence microscope for carrying out said method has a t of filter slides (20) clo to the aperture diaphragm (5) which t consists of individually displaceable, cloly spaced apart filter slides (21; 21a, 21b) with
lective filters (23; 28, 29, 31, 32) with a continuously adjustable transmission degree for every excitation band and with at least one free opening (22). The invention also relates to various advantageous embodiments of the t of filter slides (20).
申请人：LEICA MICROSYSTEMS WETZLAR GMBH
地址：Ernst-Leitz-Stras 17-37 35578 Wetzlar DE
国籍：DE
代理机构：Reichert, Werner F., Dr.
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