MICROBIOLOGICAL ANALYSIS - INCUBATION
ANAEROBIC, CARBON DIOXIDE PURGED INCUBATION
(using the Mart Anoxomat)
微生物分析-厌氧培养，二氧化碳净化培养（使用Mart Anoxomat）
1 SCOPE范围
roarAn incubation technique for the lective cultivation of anaerobic, facultatively anaerobic and奖品
microaerophilic microorganisms in petri dishes.
此培养技术适用于选择性培养培养皿中厌氧、兼性厌氧以及微需氧微生物。
2 FIELD OF APPLICATION韩语教程应用范围
Anaerobic incubation is applicable to the detection of spoilage microorganisms in yeast and
beer.
厌氧培养用于检测酵母与啤酒中的有害微生物。arizona
3 PRINCIPLE原理
The petri dishes are placed in an airtight container such as an anaerobic jar and the atmosphere in thewole container is replaced by a mixture of hydrogen (H2), carbon dioxide (CO2) and nitrogen (N2).
The container is then incubated at an appropriate temperature for the specified time interval.
培养皿放在密闭容器中，如厌氧罐与氢气、氧气以及氮气混合气体取代容器中的空气。然后容器放在适宜的温度下按规定时段培养。
4 REAGENTS试剂
4.1 Anaerobic indicator (BBL 270504 or Oxoid, BR0055)
厌氧指示剂
4.2 Anaerobic catalyst (Palladium-coated aluminium pellets) (Mart, CA0000, package of 6 pcs)
厌氧催化剂
5 APPARATUS仪器
5.1 Mart jar (Mart, e.g. AJ9023).
Mart罐
Or: Anaerobic jar (BBL, Catalogue No. 60627) with gas tight cover and inlet/outlet valve
或厌氧罐，带密封盖和进出口阀。
5.2 Anoxomat anaerobic environment generator (Mart, basic system AN2CTS)
2011河北中考数学
months
Anoxomat厌氧发生器
5.3 Petri dish support rack. (optional, Mart, e.g. PH1040)
补课一对一
可叠放培养皿
5.4 Incubator, a thermostatically controlled cupboard
培养箱，恒温控制箱
5.5 Optional: thermal printer to document, control and retrace the sample processing history
可选择：热敏打印机，管理以及追溯样品处理历史。
6 PROCEDURE程序
The Mart Anoxomat system is operated according to the manufacturers instructions.
In short: place the petri dishes in the anaerobic jar or Mart jar. Couple the jar to the Mart
Anoxomat and switch on the system by pressing either the anaerobic or microaerobic button. In the former ca, the jar must contain an anaerobic catalyst to ensure conversion of residual oxygen to water. The desired atmosphere is achieved automatically by purging with a gaous mixture of 80-85% N2, 10% CO2 and 5-10% Hcurity什么意思2. This takes approximately 2 minutes. Disconnect the anaerobic/mart jar.
The anaerobic/Mart jar may be incubated in the incubator at the appropriate temperature.
Mart Anoxomat系统根据厂家说明操作。
总之：将培养皿放在厌氧罐或Mart罐中。连接罐与Mart Anoxomat，通过按下厌氧或微需氧按钮启动系统。前一种情况，罐必须包含厌氧催化剂确保将残余氧气转化为水。通过用80-85% N2, 10% CO2 以及 5-10% H2混合气体净化获得所需的大气。所需的时间2分钟。断开厌氧/教育部不建议占用假期补课mart罐。厌氧/mart罐放进培养箱在适宜的温度下培养。
