Grapevine virus A variants of group II associated with Shiraz dia in South Africa are prent in plants affected by Australian Shiraz dia, and have also been detected in the USA 期刊名称： PLANT PATHOL
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作者： Goszczynski,DE,Habiliconsider
作者机构： [Goszczynski
朝令暮改年份： 2012年
期号： 第-期
关键词： Grapevine virus A;RT-PCR;Shiraz dia;single-strand conformation
polymorphism;variants
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摘要：Primers were designed for RT-nested PCR amplification of the highly
variable 293-nt fragment from the 5' terminal part of the Grapevine virus A (GVA)melon
astronaut是什么意思replica gene, specific to South African variants of molecular groups I and II. This
dinosaursnew technique, along with RT-PCR for simultaneous amplification of variants of
conflictiongroups I, II and III, as well as cloning of amplicons, single-strand conformation
polymorphism (SSCP) analysis of clones and quencing, were ud to investigate
the populations of variants infecting 16 local Shiraz grapevines with different Shiraz
dia (SD) status. The techniques were also ud to study variants in GVA-infected
grapevines from Australia and the USA. The Australian grapevines included ven
2011湖北高考数学plants of cvs Shiraz and Merlot affected by Australian Shiraz dia (AuSD), and
one plant of cv. Crimson
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