中国循证心血管医学杂志2018年10月第10卷第10期 Chin J Evid Bad Cardiovasc Med,October,2018,Vol.10,No.10• 1212 •• 论著 •
BRD7对过氧化氢处理的心肌细胞线粒体膜电位和胞浆中
Cyt C蛋白水平影响研究
法语在线学习李春晓1，李艳1，张静静2，张勇涛1，杨杰书3
基金项目：青岛市医药科研基金资助(2015-WJZD041)
作者单位：1 266000 青岛，青岛中心医院心血管内科;2 266000
青岛，青岛中心医院心职业病科;3 266000 青岛，青岛中心医院心查体
中心
通讯作者：杨杰书,E-mail:Yang0972********@ doi：10.3969/j.issn.1674-4055.2018.10.16【摘要】目的 研究溴区包含蛋白7（BRD7）对过氧化氢处理的心肌细胞线粒体膜电位和胞浆中细
胞色素C（Cyt C）蛋白表达影响。方法 以real-time PCR和Western blot方法检测过氧化氢处理后的心
英语应用文写作肌细胞中BRD7转录和表达水平。用BRD7 shRNA慢病毒和shRNA control慢病毒感染心肌细胞，以real-time PCR和Western blot方法检测过氧化氢条件下干扰效果。MTT测定细胞增殖活性，流式细胞术测定细胞凋亡情况，JC-1法检测线粒体膜电位，Western blot方法检测胞浆中Cyt C蛋白水平。结果 过氧化氢处理后的心肌细胞中BRD7转录和表达水平升高。BRD7 shRNA慢病毒能够下调过氧化氢条件下心肌细胞中BRD7的表达水平。过氧化氢降低心肌细胞增殖活性，诱导心肌细胞凋亡，降低线粒体膜电位，增加胞浆中Cyt C蛋白水平。下调心肌细胞中BRD7表达可以拮抗过氧化氢对心肌细胞增殖活性、凋亡、线粒体膜电位及胞浆中Cyt C蛋白水平影响。结论 下调BRD7提高过氧化氢条件下心肌细胞线粒体膜电位，降低胞浆中Cyt C蛋白水平，抑制细胞凋亡。
【关键词】线粒体膜电位；溴区包含蛋白7；心肌细胞；过氧化氢年月日英文
【中图分类号】R542.2 【文献标志码】 A 【文章编号】1674-4055(2018)10-1212-04
拖车费英文Influence of BRD7 on mitochondrial membrane potential and cytoplasmic Cyt C protein expression in cardiomyocytes treated with hydrogen peroxide Li Chunxiao *, Li Yan, Zhang Jingjing, Zhang Yongtao, Yang
slep山西培训学校Jieshu. *Department of Cardiovascular Medicine, Qingdao Central Hospital, Qingdao 266000, China.
ipbCorresponding author: Yang Jieshu, E-mail: Yang0972********@
[Abstract ] Objective To study the influence of bromodomain-containing protein 7 (BRD7) on mitochondrial membrane potential and expression of cytoplasmic cytochrome C (Cyt C) in cardiomyocytes treated with hydrogen peroxide. Methods The transcription and expression level of BRD7 in cardiomyocytes after treated with hydrogen peroxide were detected by using real-time fluorescence quantitative polymera chain reaction (RT-PCR) and Western blotting assay. The cardiomyocytes were infected with BRD7 shRNA lentivirus and shRNA control lentivirus, and interventional effects in hydrogen peroxide conditions were detected by using RT-PCR and Western blotting assay. The cardiomyocyte proliferation activity was detected by using methyl thiazolyl tetrazolium test (MTT), cardiomyocyte apoptosis was determined by using flow cytometry, mitochondrial membrane potential was detected by using JC-1 method, and level of cytoplasmic Cyt C protein was detected by using Western blotting assay. Results The transcription and expression level of BRD7 in cardiomyocytes were incread after treated with hydrogen peroxide. The expression level of BRD7 shRNA lentivirus in cardiomyocytes was down-regulated in hydrogen peroxide conditions. The cardiomyocyte proliferation activity was decread, cardiomyocyte apoptosis was induced, mitochondrial membrane potential was reduced and level of cytoplasmic Cyt
C protein was incread by hydrogen peroxide. The cardiomyocyte proliferation activity, cardiomyocyte apoptosis, mitochondrial membrane potential and level of cytoplasmic Cyt C protein were antagonized by down-regulated by BRD7. Conclusion Down-regulated BRD7 can rai mitochondrial membrane potential in cardiomyocytes in hydrogen peroxide conditions, reduce level of cytoplasmic Cyt C protein, and inhibit cardiomyocyte apoptosis.
[Key words ] Mitochondrial membrane potential; Bromodomain-containing protein 7; Cardiomyocytes; Hydrogen peroxide 心血管系统疾病的发病率和致死率在世界范
围内位居各种疾病的首位，心血管系统是机体组beer是什么意思
dominant织器官血液供应的基础，其可以为组织器官提供
营养物质和氧气[1]。缺氧缺血、高糖、动脉粥样硬化等都会引起心血管系统氧化损伤，而心肌细胞损伤是心血管系统损伤发生的关键[2-4]。溴区包含蛋白7（bromodomain-containing protein 7，BRD7）是溴区包含蛋白家族成员，其具有高度保守性，在脑组织、结肠、心脏等组织中广泛表达，能够通过结合乙酰化的组蛋白调控基因的转录，在肿瘤发生中发挥抑制作用，能够促进细胞
deduct
