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From: Methods in Molecular Biology: Drosophila:Methods and Protocols
Edited by: C. Dahmann © Humana Press Inc., Totowa, NJ
Appendix
Phenotypic Markers in Drosophila
ast
Jennifer Childress and Georg Halder
426Childress and Halder
how oldPhenotypic markers in Drosophila:wt,wild type; w,white; Me,Moiré; Roi, Roughoid; L,Lobe; Dr,Drop; Gla,Glazed; B,Bar; Cy,Curly; Hu,Humeral; y,yellow; e,ebony; sn,singed; Sb,Stubble; Sco,Scutoid; Tb,Tubby; Bc,Black cells; Sp, Sternopleural; Ser,Serrate; Ubx,Ultrabithorax.Photographs were taken on a Leica Z16 using the In-Focus software from Meyer Instruments,Houston,Texas. G raphic production by Leisa McCord.
Phenotypic Markers427
mansion
428Childress and Halder
A
Acousto-Optical Tunable Filters (AOTF),225
Akaline Phosphata (AP),211,289Anesthetics,29–31,243,246,248Apple-juice plates,e Fly food AP,e Akaline Phosphata aRNA,amplified RNA,304
homework的音标
attB ,bacterial attachment site,190–191attP ,phage attachment site,190–191Axelrod equation,230
B
enhancementBacterial artificial chromosome (BAC),191,407
BAC,e Bacterial artificial chromosome Balancer chromosome,38–40
Balancers,e Balancer chromosome BCIP,e 5-bromo-4-chloro-3-indolyl-phosphate
5-bromo-4-chloro-3-indolyl-phosphate (BCIP),211
C
Carbon dioxide,29–31,361Canton S,40
Cell lines,391–424applications,393BG2-c2,394BG3-c1,394
cell size,measurement of,413
pioneeringcell DNA content,measurement of,413Clone 8 cells,393culture media,395
DNA staining of S2 cells,377,384dsRNA,407
introduction into cells,409synthesis,408
equipment,396–398
fly extract,preparation of,401frozen stocks,preparation of,403gene silencing,e RNA interference harvesting of S2 cells,377,384immunolabelling,399,412–413Kc cells,392,393,394live imaging,411–412igbt是什么
witnessing
maintenance,guidelines for,400,402mbn-2 cells,393,394model system,394
stubbornly
RNA and protein extraction,400,414–415
RNA interference,400
bill mechanichigh throughput cell-bad screen in 384-well plates,410
in S2R +cells and 24-well dishes for microscopy,409S2 cells,392,393,394S2R +cells,394
Schneider cells,392–394rum,395
sterility,general guidelines,400storage,403
thawing cells from frozen stocks,403transfection
Baculovirus infection,404Calcium phosphate/DNA precipitates,404electroporation,404reagents,395,398stable transfection,406
lection of stably transformed cells,398vectors,399,406
transient transfection,405websites,392
Cell culture,e Cell lines
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