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英文在线翻译中文Brefeldin A(BFA) solution
Product Information
Contents: Brefeldin A(BFA) solution
Catalog Number: CS0003
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Concentration: Brefeldin A(BFA) (4mg/ml) in Ethanol Volume: 60ul
mia farrowHandling Conditions: Wear suitable personal protective equipment and gloves.
Temperature Limitation: Store at -20°C. For long-term storage, product can also be stored at less than or equal to -70°C. Protect from light.kawa
幽默Description Brefeldin A is an inhibitor of intracellular protein transport. Incubation of cells in culture with Brefeldin A leads to blockade of protein transport to the Golgi complex (GC) and accumulation of proteins in the endoplasmic reticulum (ER). Addition of Brefeldin A during the last hours of in vitro activation of cells results in enhanced detection of intracellular cytokines. Brefeldin A is effective for enhanced detection of a majority of mou and human intracellular cytokines; Suggested working concentration is 2-20ug/ml. however, it is advid that the investigators evaluate the u and efficacy of this reagent as well as other protein transport inhibitors such as Monensin in their specific assay system. Applications Reported The BFA solution has been reported for u in flow cytometric analysis.
Applications Tested The PMA solution in prent of Ionomycin has been tested by treatment of mou splenocytes for 5 to 18 hours in combination with the Protein Transport Inhibitor (BFA or Monensin) followed by intracellular staining and flow cytometric analysis of IFNγ. This product is formulated with a solvent that will not freeze at recommended storage temperatures. It is recommended that this product be kept tightly aled and cold at all times to avoid excess evaporation.
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