托尔斯泰简介中国实用医刊2019年9月第46卷第18期Chine Journal of Practical Medicine,Sep.2019,Vol.46,No.18・7・
不同浓度左旋肉碱对人精子冷冻过程中DNA碎片
与线粒体膜电位及顶体反应的影响
程兰兰谭丽冯宗刚马丽影万利静
郑州大学第二附属医院生殖中心450014
通信作者：谭丽,Email:
【摘要】目的探讨精子冷冻过程中不同浓度左旋肉碱对精子DNA碎片指数(DFI)、线粒体膜电位(MMP)、
tnscompounds顶体反应(AR)的影响。方法随机选择2017年6月至11月于郑州大学第二附属医院就诊的患者40例，患者知武汉设计学校
情同意后进行实验。采用手淫法留取精液，所有标本充分混匀后均分为5组(每组40例)。A组：冷冻前精液组；
B0组：冷冻保护剂组；B5组：添加5mmol/L左旋肉碱组；B10组：添加10mmol/L左旋肉碱组；B20组：添加
20mmol/L左旋肉碱组。采用液氮蒸汽法冷冻精液,48h后复苏。采用计算机辅助精液分析系统检测各组精子的
前向运动(PR)、活力；应用流式细胞仪检测各组精子的DFI.MMP以及AR C结果冷冻后精子PR、活力、MMP均
低于冷冻前，精子DFI高于冷冻前；四个冷冻组间PR、活力比较差异未见统计学意义(P>0.05)；DFI依次为：B10
<B20<B5<B0，其中B10和B20组低于B0和B5组；MMP依次为：B0<B5<B10<B20，其中B10和B20组高于
B0和B5组，差异有统计学意义(P<0.05)；四个冷冻组间AR比较差异未见统计学意义(P>0.05)o结论精液
冻存会造成精子的损伤，降低精子PR、活力和MMP，增加精子DFI；精子冷冻过程中添加10mmol/L或20mmol/L
左旋肉碱会降低精子DFI，减轻MMP的下降，提高精子的冻存效果。因此，10mmol/L的左旋肉碱可以作为保护精
子的有效成分应用到人精液冷冻中。
【关键词】左旋肉碱；精子冷冻；DNA碎片；线粒体膜电位；顶体反应
神曲中国菜基金项目：河南省科技厅科技攻关项目(162102310207；142300410240)
DOI：10.3760/cma.j.issn.1674-4756.2019.18.002hospital是什么意思
Effects of L-carnitine in different concentrations on DNA fragmentation,mitochondrial membrane potential and acrosome reaction during human sperm cryoprervation
Cheng Lanlan,Tan Li,Feng Zonggang,Ma Liying,Wan Lijing
Reproductive Center,the Second Affiliated Hospital of Zhengzhou University,Zhengzhou450014,China Corresponding author：Tan Li,Email:
[Abstract]Objective To investigate the effects of L-camitine in different concentrations on
DNA fragmentation index(DFI),mitochondrial membrane potential(MMP)and acrosome reaction
负担英文(AR)during human sperm cry o prervation.Methods Forty patients were randomly lected from the
June2017to November2017in the Second Affiliated Hospital of Zhengzhou University.Experiments
were carried out on the patient connt.In addition,the men samples were collected by masturbation
trunk是什么意思
翻云覆雨的意思是什么method.All men samples were fully mixed and divided into five groups:group A with fresh men as
control,group B0with conventional cryoprotectant,group B5with5mmol/L L-camitine,group B10with
10mmol/L L-camitine,group B20with20mmol/L L-camitine.The men samples were frozen by
liquid nitrogen vapor,and then were thawed48h later.Computer-aided sperm analysis was ud to detect
the progressive motility(PR)and viability of spermatozoa before and after cryoprervation.Flow cytometry was ud to detect DFI,MMP and AR in each group.Results The PR,motility and MMP
after freezing were significantly lower than tho before freezing.The sperm DFI was higher than that
before freezing.There was no difference in the likelihoods of the PR on motility between four frozen groups职称英语新增文章
(P>0.05).The sperm DFI of spermatozoa were as follows:B10<B20<B5<B0,in which B10group
