Process for preparing broad host range small plasm

更新时间:2023-07-04 19:26:47 阅读: 评论:0

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专利名称:Process for preparing broad host range small plasmid rings as cloning vehicles 发明人:Ronald H. Oln
申请号:US06/312409
申请日:19811019finishes
公开号:US04376164A
公开日:rootkit
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摘要:The prent invention relates to novel, broad bacterial host range small plasmid deoxyribonucleic acid rings which rve as cloning vehicles for DNA fragments, particularly tho parated from other plasmid rings or from chromosones, recombined with the small plasmid rings and to the process for recombining the plasmid rings and to process for transferring them between host bacteria. In particular, the prent invention relates to the aggregate plasmid ring RP1/pRO1600, to pRO1600 and plasmid ring derivatives thereof, particularly including pRO1601; pRO1613 and pRO1614, all of which are carried for reference purpos in Pudomonas aeruginosa ATCC 15692 (also known as strain PAO1c) and are on deposit at the Northern Regional Rearch Laboratories (NRRL) of the U.S. Department of Agriculture at Peoria, Il. The plasmid ring RP1 (also known as R1822) is deposited in Pudomonas aeruginosa NRRL-B-12123 (and is a known plasmid ring). The pRO1600 portion of the aggregate is a new plasmid ring. The novel small plasmid rings are particularly uful for recombinant genetic manipulation wherein the DNA fragments are introduced into the plasmid rings to produce uful, cloneable characteristics in the host bacterium, particularly chemical generating characteristics.培训日语
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申请人:MICROLIFE TECHNICS, INC.代理人:Ian C. McLeod
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