Species-specific detection and quantification of common barnacle larvae from the Japane coast using quantitative real-time PCR.
期刊名称： Biofouling
网报>funny mud pee翻译作者： Noriyuki Endo,Kana Sato,Kiyotaka Matsumura,Erina Yoshimura,Yukiko男士头发护理
Odaka,Yasuyuki Nogata
作者机构： Environmental Science Rearch Laboratory,Civil Engineering Rearch and Environmental Studies
年份： 2010年
期号： 第7-8期
walt disney
surpri什么意思>hiroko关键词： barnacle; larvae; quantitative detection; quantitative real-timethetimes
polymera chain reaction; species; identification; 12S ribosomal RNA
英语口试自我介绍摘要：Species-specific detection and quantification methods for barnacle larvae using quantitative real-time polymera chain reaction (qPCR) were developed. Species-specific primers for qPCR were designed for 13 barnacle species in the mitochondrial 12S ribosomal RNA gene region. Primer specificity was examined by PCR using template DNA extracted from each of the 13 barnacle species, other unidentified barnacle species, and field collected zooplankton samples. The resulting PCR products comprid single bands following agaro gel electrophoresis when the templates corresponded to primers. The amplifications were highly species-specific even for the field plankton samples. The field plankton samples were subjected to qPCR assay. The calculated DNA contents for each barnacle species were cloly correlated with the number of larvae
caligulatodo内容由中国教育图书进出口有限公司引进
