1. Purpo: To ensure the smooth operation of GC-2010 plus gas chromatograph by establishing
a standard operating procedure.
2. Scope: This SOP applies to the operation of GC-2010 plus gas chromatograph.
3. Responsibility: QC laboratory technician shall be responsible for this SOP and the director of laboratory assumes responsibility of supervision over and check of the effective implementation of this SOP.
4. Content:
4.1 Starting up
4.1.1 Select appropriate gas chromatographic column according to the standard requirements of the sample to be measured, and replace the chromatographic column.
4.1.2 Obrve whether the silica gel inside the all-in-one machine of nitrogen, hydrogen and air changes color or not and whether the liquid level is normal or not. Open the all-in-one machine of nitrogen, hydrogen and air in ca of no abnormality.最新大片推荐
4.1.3 Open the switching valves of N2, H2 and Air on the all-in-one machine of nitrogen, hydrogen and air. Obrve the pressure of N2 in the all-in-one machine of nitrogen, hydrogen and air. Start the GC-2010 Plus gas chromatograph when the pressure reaches more than 60MPa.pae
4.1.4 Enter the obrving interface by clicking the button “Moint” on the host.
4.1.5 Open the computer workstation. Long sound beeping reprents that the computer is online successfully.
4.1.6 Open the saved original method file or ret new parameters, including chromatographic column, injection port mode (split flow), column temperature, detector and end time (temperature: column temperature ＜injection port ＜detector). Auto-ignition is off. Method of creating new file: Click “Save method file as” to save the method under the method file, then click “Download”; Click “Download” directly in ca of the saved original method file.lama
4.1.7 Click “Start GC”.
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4.1.8 Obrve the instrument state on the right of the workstation or the host display screen. Wait for the flow rate of carrier gas and the temperature of all components reaching the t values.
4.1.9 Select “Ignition” on the right of the workstation as status of “ON”to ignite. Beep sound appears. And the host of GC-2010 Plus gas chromatograph displays real flame image.
4.1.10 Prepare to inject the sample after the baline is stable and the instrument gets ready.
4.2 Sample Analysis
4.2.1 Click “Single analysis”. Set name, ID and save path of the sample in the dialog box of “Sample registration”. Check “Auto increment”. Then click “Ok”.
4.2.2 When the dialog box of “Start to collect data” appears, inject the sample with micro-injector from the injection port and press the button “Start” on the host to conduct data collection.
4.2.3 The instrument will stop automatically and save the data file according to the end time t in the detector. If you want to stop the data collection in advance, you can press the button “Stop”after the peak appears and becomes stable.
接电话用英语怎么说4.2.4 The next single analysis can be performed when the instrument is in the state of “Be ready”. Make analysis and data collection on the standard sample and the sample to be tested in this way successively.
4.3 Data Processing
4.3.1 Open “GC Solution” on the desktop. Click “Re-analysis” in “Processing tool” and enter the data processing interface.
4.3.2 Find out the file to be procesd in the destination folder. Click “Manual integration”in ca ther
e are impure peaks. Select appropriate processing method in the manual integration bar and get corresponding data including “retention time”, “peak area”, “height” and “concentration”.
Then calculate the required results according to the requirements.
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4.4.1 Click “Data report”in “Data processing”. Open the existing report format file or lect “Create a new report format file”in “File”. Inrt appropriate item information including “chromatogram”, “sample information” and “peak table” as required.
4.4.2 Select the file to be printed in “Data” and drag it into “Report generation”. Then print it.谷歌英文版
英语职称考试成绩查询4.5 Shut down the Instrument
4.5.1 Set the temperature of column, injection port and detector as indoor temperature. Click “Download” and check “Off” as the status of “Ignition”. Then wait for cooling.
txu4.5.2 Click “Stop GC” and clo the workstation after the temperature of detector, injection port and column falls below 50℃. Beeping sound reprents that the workstation has disconnected with the GC host.
4.5.3 Turn off the computer and the host. And cut off the power.
4.5.4 Switch off the all-in-one machine of nitrogen, hydrogen and air. Press the Air vent switch on the all-in-one machine of nitrogen, hydrogen and air to remove the residual gas. Cut off the power after the nitrogen is exhausted.
5. Supplementary Provisions
Referenced Records List of this Document
6.This document is drafted by the laboratory, reviewed by the director of laboratory and approved by the chief of quality department.
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