The Nijmegen modification of the Bethesda assay for factor VIII:C inhibitors: improved specificity and
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补英语期刊名称： Thrombosis & Haemostasis
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作者： VERBRUGGEN,B
年份： 1995年
期号： 第2期bear的用法
关键词： Humans;Hemophilia A;Factor VIII;Antibodies;Immunoassay;Logistic
Models;Sensitivity and Specificity;Reproducibility of Results;Predictive Value of
Tests;Hydrogen-Ion Concentration
南京英语培训班摘要：Antibodies against factor VIII coagulant activity can appear in haemophiliacs who are treated wit
pirateh factor VIII preparations but also spontaneously in non-haemophiliacs. The Bethesda assay is the most commonly ud method to detect the antibodies, but it lacks specificity especially in the lower range resulting in unreliable data. Two modifications are propod and tested to resolve the imperfections: 1. Buffering the normal plasma ud in the assay- and control mixture with 0.1 M imidazole to pH 7.4. Replacing the imidazole buffer in theperth是什么意思
control mixture by immunodepleted factor VIII deficient plasma. The modifications allow better discrimination between positive and negative samples and improve reliability.
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