ndo>目录英文
Molecular specificity of two commercial enzyme linked immunosorbent assays for human immunodeficiency virus antigens.伤停补时
against的用法期刊名称： Journal of Clinical Pathology
作者： Brown, K E, Shanson, D C, Mascagni, P, Coates, A R
年份： 1992年
期号： 第12期eastman kodak
摘要：Only "fair" agreement has been shown between the Abbott and DuPont enzyme linked immunosorbent assays when ud for the detection of human immunodeficiency virus (HIV) antigen in rum samples from asymptomatic HIV antibody positive homoxual men. To investigate the discrepancies between the two ELISA results, further experiments were performed. The rabbit detector antibody solutions of both tests were western blotted and showed that the DuPont test was specific for p24; the Abbott detector antibody had bands for p18, p41-43, gp120 as well as p24. By using dilutions
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dhp>cargoesof a known amount of HIV antigen, the Abbott test could detect 20 pg/ml p24; the DuPont test could detect 30 pg/ml
p24. The DuPont test was also more nsitive than the Abbott test at detecting a synthetic 104mer peptide of p24. Within the 104mer quence
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