Final Exam (part I) (英语试卷的参考答案)

更新时间:2023-06-23 22:21:50 阅读: 评论:0

Biochemistry Test part one
口福的意思
祝福用英语怎么说Name                   Student ID                   Score dismissal           
I
II
III
IV
Total
I  Write the Structures and state their applications respectively in Biochemistry. (5 x 4 = 20)
Number
Structure
Applications
1
dicyclohexylcarbodiimide
多肽合成中氨基酸单体的活化试剂或缩水试剂。
2
cyanogen bromide
特异性断裂甲硫氨酸C-端肽键
3
aspartyl protea active site
两个天冬氨酸
构成天冬氨酸蛋白酶的活性位点。
4
single ladyATP
能量分子,磷酸基团供体
5
cAMP
蛋白激酶A的活化信使
II  Matching Questions (30 x 1 = 30)
U the items listed in the table to answer questions 1-15. Not all of the answers will be ud.
survivedalternative是什么意思
A
B
C
D
E
1
zymogen activation
phosphorylation
kinas
liga
phosphatas
2
evolutionary tree
convergent
divergernt
thalasmia
sickle-cell
3
centrifugation
ion exchange
affinity
gel filtration
precipitationlithium
4
synonyms
anonymes
cleft
inner
surface
5
competitive
noncompetitive
uncompetitive
feedback
feedforward
6
turnover number
covalent bond
salt bridge
van der Waals
asparagine
7
cysteine
洛基英语histidine
arginine
aspartate
valine
8
γ-carboxyglutamate
tyrosine
lysine
rine
hydroxyproline   
9
ATP
cAMP
cGMP
homotropic
heterotropic
10
primary
condary
兔子障碍赛
tertiary
negative
positive
11
restriction endonucleas
orthologs
paralogs
adult
fetal
12
superoxide
Bohr effect
SD quence
gla domain
fingerprint
13
Ramachandran
Sanger
Chargaff
myoglobin
metmyoglobin
14
isozymes or isoenzymes
Ribozyme
allosteric
cooperative
noncooperative
15
stopped-flow
RNAi
gene disruption
Zn2+
Mg2+
16
rever trancripta
DNA polymera
RNA polymera
Fe+2
Fe+3
17
gene expression
gene transcription
mRNAs
tRNAs
rRNAs
18
site-directed mutagenesis
elagation
relea
expression
cloning
1. Some inverted repeat quences or palindromic quences can be specifically cleaved by ____11A_________.
2. The process by which very different evolutionary pathways lead to a protein with similar function is referred to as __2B___ evolution.
3. Under normal conditions, the iron of heme in myoglobin and hemoglobin is in the ___16D____ oxidation state.
4. Carbon dioxide reacts with the amino terminal groups of hemoglobin to form carbamate groups, which carry a ___10D___ charge. 
5. In normal adult hemoglobin, HbA, the β6 position is a glutamate residue, whereas in sickle-cell hemoglobin, HbS, it is a __7E___ residue. 
6. The T-state of hemoglobin is stabilized by a salt bridge between β1 Asp 94 and the C-terminal ____7B____ of the β1 chain.
7. In trypsin, the specificity pocket contains a/an ____7D____ residue that binds to amino acid residue carrying positive charge of the substrate.
8. In proteas such as papain, a _____7A___ residue is activated by hydrogen-bonding to a histidine residue.
9. Multiple forms of homologous enzymes within the same organism that catalyze the same reaction but with different kinetic properties are ____14A____.
10. The effects of substrates on allosteric enzymes are referred to as _____9D_____ effects.
11. ___9A___ is the most common donor molecule in phosphorylation reactions catalyzed by protein kinas.
12. α helices, β sheets, and turns are referred as__10B___ structure of proteins.
13. The purification bad on the attraction of the protein for a particular chemical group is ___3C__ chromatography.
14. The information-carrying intermediates for translation are _17C_.
15. The person who noted the A:T and G:C ratios in DNA are approximately 1:1 is _13C_.
16. Codons that specify the same amino acids are termed __4A__.
17. A pattern of DNA fragments by restriction digestions can rve as a _12E  of a particular DNA molecule.
18. Glutamine substitution with __6E__ is an example of a conrvative amino acid change.
19. The decread production of one of the subunits of hemoglobin can cau _2D__.
20. Two α chains and two γ chains are ud to constitute ___11E___ hemoglobin.
21. The kcat is often referred to as the _6A__.
22. Enzymes that do not obey Michaelis-Menten kinetics are _14C__.
23. The activity of carbonic anhydra requires metal ion __15D__.
24. We can u __18A___ to determine structure/function relationships in enzymes.
25. We usually refer the inhibition of the first step by the final product of the pathway as__5D__inhibition.
26. Activation of prothrombin requires modified amino acid __8A__ in gla domain.
27. The enzyme ud to create DNA from RNA is   16A  .
28. Removal of phosphates from proteins is catalyzed by protein __1E__.
29. Ω, β turns and loops are often found on   4E  of proteins.
30. Blood clotting cascades are controlled by __1A_. 

III Fill in the Blanks (2 x 10 = 20)
1. The aromatic amino acids in proteins are   Phe, Tyr, and Trp        .
2. During protein purification, both   测定比活性和回收率  are necessary each step.
3. Monoclonal antibodies are made by   生产抗体的B细胞与小鼠骨髓瘤细胞融合    .
4. Semi-conrvative replication of DNA was confirmed by M. Melson and F. W. Stahl experiment(s).
5. Distal histidine in hemoglobin donates   hydrogen bond  to oxyhemoglobin complex, which inhibits the oxidation of the iron to the ferric state.
6. α-hemoglobin is stabilized by   AHSP  .
understandable7. The meaning of Vmax catalyzed by an enzyme is   所有活性位点都与底物结合时的反应初速度  .
8. An affinity label of an enzyme is   有反应活性基团的底物类似物                .

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