种常⽤培养基配⽅
146种常⽤培养基配⽅
THE COMPOSITION OF MEDIA
培养基及成分
1. Acetobacter Medium (醋酸菌培养基)
Gluco (葡萄糖) 100g Yeasst extract (酵母膏) 10g英汉字典
CaCO3 20g Agar (琼脂) 15g
Distilled water (蒸馏⽔) 1000ml Adjust (调) pH to
适⽤范围：恶臭醋酸杆菌混浊变种
2. Nutrient Agar (营养⾁汁琼脂)
Pepton (蛋⽩胨) 5g Beef extract (⽜⾁膏) 30g
NaCl 5g Agar (琼脂) 15g
Distilled water (蒸馏⽔) Adjust (调) pH to
[Note]：When cultivation of Bacillus，5mg of to may be added .
It is favorable to promote spore formation .
牛津小学英语
适⽤范围：产⽓⽓杆菌、粪产碱杆菌、蜡状芽孢杆菌、蜡状芽孢杆菌蕈状变种、地⾐形芽孢杆菌、巨⼤芽孢杆菌、多粘芽孢杆菌、尘埃芽孢杆菌、短⼩芽孢杆菌、嗜热脂肪芽孢杆菌、枯草芽孢杆菌、枯草芽孢杆菌深⿊变种、苏云⾦芽孢杆菌、苏云⾦芽孢杆菌蜡螟亚种（青⾍菌）、苏云⾦芽孢杆菌⼽尔斯德变种、苏云⾦芽孢杆菌猝倒亚种、产氨短杆菌、黄⾊短杆菌、⾕氨酸棒状杆菌、北京棒杆菌、⼤肠埃希⽒菌（⼤肠杆菌）、铜绿假单胞菌（绿脓杆菌）、凸形假单胞杆菌、荧光假单胞菌、弯曲假单胞菌、恶臭假单胞菌、假单胞杆菌、藤黄⼋叠球菌、亚黄⼋叠球菌、尿素⼋叠球菌、⾦黄⾊葡萄球菌、运动发酵单孢菌
3. Azotobacter Medium (固氮菌培养基)
KH2PO4 K2HPO4
Trace(微量) Yeast axtract(酵母膏)
Mannitol(⽢露醇) 20g FeCl3 Tract(微量)
Distilled water (蒸馏⽔) 1000ml Agar (琼脂) 15g
Adjust (调) pH to
适⽤范围：固氮菌、胶质芽孢杆菌
4. Corn Meal Medium (⽟⽶粉培养基)
cinema什么意思Maize flour (⽟⽶粉) 5g Peptone (蛋⽩胨)
Gluco (葡萄糖) 1g Tap water (⾃来⽔) 1000ml
英语作文翻译[Note]:Boil the mixture in autoclave at 121℃for 1 hr. distribute the medium into 18ⅹ18 mm tubes , each contains 10 ml of the liquid , then autoclave at 121℃for 1 hr . again (15磅蒸煮1⼩时，分装⼊18ⅹ18毫⽶试管，每管深度达6厘⽶。15磅再次灭菌15⼩时。)
5. Lactic-bacteria Medium I (乳酸菌培养基I )
Yeast extract (酵母膏) Peptone (蛋⽩胨)
Gluco (葡萄糖) 10g KH2PO4 2g
Tomato juice (西红柿汁) 100ml Tween (吐温) 80
Distilled water (蒸馏⽔) 900ml pH
适⽤范围：植物乳杆菌（胚芽乳杆菌）、嗜热乳酸链球菌
6. Lactic-bacteria Midium Ⅱ(乳酸菌培养基Ⅱ)
Lacto-cain peptone (乳酪蛋⽩胨) 10g Beef extract (蛋⽩胨) 10g
Yeast extract (酵母膏) 5g Gluco (葡萄糖) 5g
Tween (吐温) 80 1g K2HPO4 2g
Na-acetate (醋酸钠) 5g Diamine citrate (柠檬酸⼆胺) 2g
ambleDistilled water (蒸馏⽔) 1000m pH
适⽤范围：植物乳杆菌（胚芽乳杆菌）
7. Peotone Gluco Yeast extract Medium PGY （蛋⽩胨、酵母膏、葡萄糖培养基）
Peptone（蛋⽩胨）10g Yeast extract （酵母膏）5g
Gluco （葡萄糖）1g Distilled water （蒸馏⽔）1L
8. Glycerol Agar （⽢油琼脂）
Peptone （蛋⽩胨）5g Beef extract （酵母膏）3g
Glycerol （⽢油）20g Top water （⾃来⽔）1000ml
Agar （琼脂）15g pH
9. Rhizobium medium （根瘤菌培养基）AS 9
Yeast eztract （酵母膏）1g Soil eztract （⼟壤浸提液）200ml
Mannitol （⽢露醇）10g Agar （琼脂）15g
Distilled water （蒸馏⽔）800ml pH
[Note]：Soil extract：Suspend 50g finely and dried gardon soil in 200ml of tap water. Autoclave at 121℃for 1 hr. Decant through cotton-cloth, filler though paper, make up volume to 200ml. Resterilize for 20 minutes at 121℃, then mixed with othringredients and distributed. (⼟壤浸提液的制法：取⼟壤50克，加⽔200毫升，15磅蒸煮1⼩时，经滤纸过滤后加⽔补⾜到200毫升。) 适⽤范围：⼤⾖根瘤菌（慢⽣型）、豇⾖慢⽣根瘤菌、花⽣根瘤菌、紫云英根瘤菌、
⼤⾖根瘤菌（快⽣型）、⼤⾖根瘤菌、豌⾖根瘤菌、苜蓿根瘤菌、⽥菁根瘤菌
10. Mannitol Agar (⽢露醇琼脂)
Yeast extract (酵母膏) 5g Peptone (蛋⽩胨) 3g
Mannitol (⽢露醇) 25g Agar (琼脂) 15g
Distilled water (蒸馏⽔) 1000ml
11. Gluco Asparagine （葡萄糖、天门冬素琼脂）
Gluco （葡萄糖）10g Asparagine （天门冬素）
K2HPO4 Water （⽔）1000ml
pH
适⽤范围：刺孢⼩单孢菌绛红变种、紫⾊⼩单孢菌（绛红⼩单孢菌）
12. Gau′s Synthetic Agar （⾼⽒合成⼀号琼脂）
KNO3 1g Soluble starch（可溶性淀粉）20g
K2HPO4
NaCl FeSO4
Agar （琼脂）20g water （⽔）1000ml
pH
适⽤范围：刺孢⼩单孢菌绛红变种、紫⾊⼩单孢菌（绛红⼩单孢菌）、⽩黄链霉菌、⽩⾊链霉菌、抗⽣链霉菌、双重轮丝链霉菌、产⾊链霉菌、烬灰链霉菌、天蓝⾊链霉菌、灭蚊链霉菌、红霉素链霉菌、青⾊链霉菌、球孢链霉菌、浅灰链霉菌、灰⾊链霉菌、吸⽔链霉菌、淡紫灰链霉菌、黄⾊长孢链霉菌、藤黄⾊链霉菌、细黄链霉菌、⿊化链霉菌、玫瑰⾊链霉菌、华美链霉菌、嗜热链霉菌、委内瑞拉链霉菌、紫⾊直丝链霉菌、紫⾊链霉菌、绿⾊链霉菌
13. Wort Agar （麦芽汁琼脂）
Dilute the world (without hop) to 12 Brix. Add 15g agar into 1000ml of the diluted word..Melt the agar by heating, then distribute the medium into tubes. Autoclave at 110 for 30 minutes. (将发酵啤酒的原料（未加酒花），稀释⾄12柏林，加琼脂15克，溶化后分装。15磅灭菌30分钟。)
适⽤范围：克鲁斯假丝酵母、郎⽐可假丝酵母、解脂假丝酵母、马其顿假丝酵母、拟热带假丝酵母、粗壮假丝酵母、皱褶假丝酵母、热带假丝酵母、产朊假丝酵母、阿舒假囊酵母、⽩地霉、果⾹地霉、地霉属、异常汉逊酵母、异常汉逊酵母变种、阿拉伯糖醇汉逊酵母、施⽒汉逊酵母、菅囊酵母、伯顿毕⾚酵母、膜醭毕⾚酵母、粘红酵母、⼩红酵母、胶红酵母、深红酵母、卡尔斯伯酵母、酿酒酵母、椰园酿酒酵母、发酵性酵母、洛格酵母、罗斯酵母、鲁⽒酵母多形鲁⽒酵母、威尔酵母、酵母、路德类酵母、栗酒裂殖酵母、掷抱酵母、贝雷丝孢酵母、⽪状丝孢酵母、糙孢曲霉、⽆壳曲霉、鲜橙曲霉、红曲霉、紫红曲霉、红⾊红曲霉、红曲霉菌
14. Potato Dextro Agar PDA (马铃薯、葡萄糖琼脂)
Potato extract (马铃薯汁) 1000ml Dextro(gluco) (葡萄糖) 20g
Agar (琼脂) 20g
[Note]: Potato extract: Slice potatoes thin. Add 1000ml of water, immediately to prevent Oxidtion of juice and boil until soft. (approximately 30min.). Filter through cotton-cloth. Autoclave at 115℃for 20 minutes.（注：取去⽪马铃薯200克，切成⼩块，加⽔1000毫升煮沸30分钟，滤去马铃薯块，将滤液补⾜⾄1000毫升，加葡萄糖20克，琼脂15克，溶化后分装，15磅灭菌30分钟。）适⽤范围：酵⽶⾯假单胞菌（酵⽶⾯黄杆菌）、⽩⾊链霉菌、烬灰链霉菌、青⾊链霉
菌、球孢链霉菌、灰⾊链霉菌、龟裂链霉菌、伞枝梨头霉、雅致放射⽑霉、棒曲霉、
⽶曲霉、出芽短梗霉、⽩僵霉、灰葡萄孢、顶头孢霉、巴西⽑壳、长刺⽑壳、橄榄包
⽑壳、反曲⽑壳、琥珀⽑壳、圆酵⽑壳、束状刺盘孢、新⽉弯孢霉、奇异翅孢壳、地英语口语mp3下载
⽣翅孢壳、串珠镰孢、尖镰孢、盘长孢菌（鲁保⼀号）、银⽩杨盘长孢、⽟蜀⿉长蠕
孢、深黄被孢霉、⼩被孢霉、多头被孢、拉曼被孢霉、葡萄⾊被孢霉、⽣⾹⽑霉、卷
枝⽑霉、两型孢⽑霉、直⽴⽑霉、球孢⽑霉、丝球⽑霉、⼤⽑霉、多型孢⽑霉、总状
⽑霉、鲁⽒⽑霉、五通桥⽑霉、⿊球漆斑菌、露湿漆斑菌、玫烟⾊拟青霉、棉铃⾍拟
青霉、拟青霉、球形⾩孢、⽩腐菌、少根根霉、华根霉、科恩根霉、戴尔根霉、⽇本根霉、⽖哇根霉、⽶根霉、点头根霉、绿穂霉、簇孢匍柄霉、雅致枝霉、康宁⽊霉、绿⾊⽊霉、黄萎轮枝孢、⼤丽花轮枝孢
15. Czapek′s Agar（察⽒琼脂）moma
Sucro （蔗糖）30g NaNO3 3g
KCl
K2HPO4 1g
Agar (琼脂) 13g Distilled water (蒸馏⽔) 1000ml
pH
适⽤范围：红霉素链霉菌、洋葱曲霉、⾦头曲霉、泡盛曲霉、泡盛曲霉烟⾊变种、亮⽩曲霉、炭⿊曲霉、⿅⽪⾊曲霉、棒曲霉、⽆花果曲霉、黄柄曲霉、黄曲霉、烟曲霉、构巢曲霉、⿊曲霉、赭曲霉、⽶曲霉、寄⽣曲霉、酱油曲霉、⼟曲霉、宇佐美曲霉、焦曲霉、杂⾊曲霉、温特曲霉、阿达青霉、产黄青霉、黄绿青霉、桔青霉、顶青霉、园弧青霉、绳状青霉、灰黄青霉、岛青霉、淡紫青霉、特异青霉、赭绿青霉、产紫青霉、娄地青霉、⼩刺青霉、荨⿇青霉、
16. Concentrated Sucro Czapek′s (浓糖察⽒琼脂)
Sucro (蔗糖) 200g NaNO3 3g
KCl
K2HPO4 1g
Agar (琼脂) 13g Distilled water (蒸馏⽔) 1000ml
适⽤范围：谢⽡曲霉
17. Synthetic Potato Medium (综合马铃薯培养基)
20%Potato extract (马铃薯汁) 1L Gluco (葡萄糖) 20g
KH2PO4 3g
Vitanib B1 (硫胺素) Trace (微量) Agar (琼脂) 15g
pH 6
适⽤范围：⽶曲霉、酱油曲霉、栖⼟曲霉、宇佐美曲霉、出芽短梗霉、⽩僵霉、灰葡萄孢、顶头孢霉、巴西⽑客、长刺⽑壳、球⽑壳、橄榄包⽑壳、反曲⽑壳、琥珀⽑壳、圆酵⽑壳、多主枝孢、束状刺盘孢、葫芦科刺盘孢、新⽉弯孢霉、奇异翅孢壳、地⽣翅孢壳、串珠镰孢、蚀脉镰孢霉、盘长孢菌（鲁保⼀号）、银⽩杨盘长孢、瓶⽑壳、⾦龟⼦绿僵菌、⽣⾹⽑霉、卷枝⽑霉、两型孢⽑霉、直⽴⽑霉、球孢⽑霉、丝球⽑霉、⼤⽑霉、多型孢⽑霉、总状⽑霉、鲁⽒⽑霉、五通桥⽑霉、⿊球漆斑菌、露湿漆斑菌、玫烟⾊拟青霉、棉铃⾍拟青霉、淡紫⾊拟青霉、拟青霉、⽩腐菌、少根根霉、根霉属的菌、华根霉、科恩根霉、戴尔根霉、台湾根霉、⽇本根霉、⽖哇根霉、⿊根霉、雪⽩根霉、少根根霉、⽶根霉、点头根霉、三宝垄根霉、根霉属的种、绿穂霉、康宁⽊霉、⽊素⽊霉、拟康⽒⽊霉、⽊霉、粉红⽊霉、绿⾊⽊霉、黄萎轮枝孢、⼤丽花轮枝孢、双孢蘑菇、⼤肥菇、发光假蜜环菌、⽊⽿、皱⽊⽿、⽑⽊⽿、⼤秃马勃、长根菇、⾦针菇、棘托⽵荪、短裙⽵荪、短裙⽵荪东北变种（暂定）、长裙⽵荪、⽩⿁笔、⽆裙⽵荪、宽鳞⼤孔菌、凸圆灵芝（⽩芝）、紫芝、灵芝（红芝，⾚芝，⽇本灵芝，韩国灵芝）、灰树花、猴头菌、⾹菇、粗⽑⽃菇、洁丽⾹菇、豹⽪菇、⽟蕈、真姬菇、安络⼩⽪伞菌、⿁⽑针、⿊柄⽪伞菌、尖顶⽺肚菌、圆锥⽺肚菌、⾼⽺肚菌、⿊脉⽺肚菌、⽺肚菌、滑菇、光帽黄伞、尖鳞黄伞、⾦顶侧⽿、榆黄磨、⾦顶磨、⽩黄侧⽿、紫孢平菇、姬菇、裂⽪侧⽿、拟囊体侧⽿（鲍鱼菇）、真线侧⽿短孢变种、真线侧⽿、扇形平菇、扁形平菇、佛罗⾥达⽆孢⼦侧⽿、佛罗⾥达侧⽿、蚝菇、糙⽪侧⽿、平菇、贝形侧⽿、鹰翅菌、肺形侧⽿、柳树菌、树窝、扁柄侧⽿、美味侧⽿、紫孢侧⽿、粉褶侧⽿、粉红侧⽿、凤尾菇、漏⽃状侧⽿、榆⼲侧⽿、⼤榆磨、侧⽿、茯苓、⿊菇
、烟⾊红菇、裂褶菌、鸡纵、⾦⽿、黄⽊⽿、银⽿、⽩⽊⽿、紫晶⼝磨、银丝菇、丝盖⼩包脚菇、草菇
18. Corn Meal Medium (⽟⽶粉培养基)
Mix corn meal with water [20:100(W/V)] well . Distribute the mixture into 100ml bottles , each bottle contains about 30ml of the mixture . Autoclave at 121℃for 30minutes . (20%⽟⽶粉装瓶，15磅灭菌30分钟。)
19. Filter Paper Medium （滤纸培养基）
（NH4）2SO4 1g KH2PO4 1g
职称英语准考证打印
NaCl
Distilled water （蒸馏⽔）1000ml A strip of filter paper （滤纸条）6ⅹ1cm （厘⽶）
20. Soybean Cake Meal Agar (黄⾖饼粉琼脂)
10% infusion of soybean cake meal (10%黄⾖饼粉浸汁) 1000ml
Gluco (葡萄糖) 10g NaCl
21. Sawdust，Wheat-bran Medium （⽊屑、麸⽪培养基）
Sawdust of broad leaf tree 75g Wheat bran 25g
Preparation：Mix the two ingredients well . Add appropriate water and blend (the amount of water added may be becided by kneading with fingers ). Fill the mixture into broad mouth of bottles . Stick a hole on the filling, put the cotton pulg in bottle mouth .
壳⽃科植物或阔叶树的⽊屑75克麸⽪25克
制法：加⽔适量充分混匀，⽔量以⼿捏时指间有⽔珠为宜，装瓶并压紧，在中⼼处扎⼀⼩洞，堵
上棉塞，20磅灭菌1⼩时。
22. Pine Block or Pine Sowdust Medium （松⽊条或松⽊屑培养基）
1. Cut 1021 cm pine biocks and immer them in 1-2% sucro solution . Alow the biocks fully absorbing suger solution .Fill the biocks into broad month of bottle . Park the bottle with paper . Auto
爱很简单英文版clave at 20 lbs for 1 hr . (将10ⅹ2ⅹ1厘⽶的松⽊条浸泡在1-2%的蔗糖⽔中。使⽊条吸⾜糖⽔，装瓶不宜过紧，⽤纸包好，20磅灭菌1⼩时。)
2. Pine Sawdust Medium
Pine Sawdust (松⽊屑) 75% Rice bran (⽶糠) 23-24%
Socro (蔗糖) 1-2% Water (appropriate)⽔（适量）
Autoclave at 20 lbs for 1 hr.(20磅灭菌1⼩时）
23. Rice Straw. Dung Medium (稻草、马粪培养基)
Dried hor dung (or cow dung) （⼲马粪或⽜粪）65%
Died straw （⼲稻草）35% Urea （尿素）%
Calcium superhosphate （过磷酸钙）% Gypsum powder（⽯膏粉）1%
Ammonium sulphate （硫酸铵）%
[Note]: Add appropriate water, pile them up and cau them fermentation. （⽔适量进⾏堆积发酵）?
24. Straw Rice Bran Medium （稻草、⽶糠培养基）
Dried straw （⼲稻草）75-80% Rice bran （⽶糠）20-25%
[Note]：Select no mouldy straw and cut them about 3 cm in length. Immer them in fresh water for 12 hr. Allow the straw fully (the amount of water added may be decided by kneading with fingers. It is right when a drop of water appeared between fingers). ( [注]：选⽆霉稻草，切成长约3厘⽶的⼩段，⽤清⽔浸泡12⼩时，使充分吸⽔，然后倾去⽔，加⽶糠和⽔，⽔量以⽤⼿捏时指间有⽔珠为宜。)
25. Rice Medium (⽶饭培养基)
Fill 10g of rice into a 50ml of Erlenmeyer flask. Add 20-30ml water. Autoclave at 121℃for 30 minutes (or fill 2g of rice into a 15ⅹ150mm tube). Add 6 ml of water. Autoclave at 121℃for 30 minutes. (取⼤⽶10克，⼊50毫升椎形瓶中，⽔20-30毫升于15磅灭菌30分钟即成，或取⼤⽶2克，装⼊15ⅹ150毫⽶试管中，加⽔6毫升，于15磅灭菌30分钟。)
26. Filter Paper Medium (滤纸条培养基)
(NH4)2SO4 1g KH2PO4 1g
Yeast Extract (酵母膏)
Distilled Water (蒸馏⽔) 1000ml
Strip of filter paper 1ⅹ7 cm (滤纸条1ⅹ7 厘⽶)
[Note]：Siddolve the above ingredients. Distribute the liqid medium into tubes (each tube contains 1ml of the broth). Autoclave at 121℃for 30 minutes. Then put one or two sterilized strips of filter paper into a tube aptically. (将上述溶液分配成溶液后分装⼊试管，每管1毫升，15磅灭菌30分钟。再将灭菌的滤纸条（1条或2条）投⼊试管中即好使⽤。)
27. Yeast Extract-Medium Extract Agar I (酵母膏、麦芽膏琼脂)
Yeast extract (酵母膏) 10g Mall extract (麦芽膏) 10g
Gluco (葡萄糖) 4g Distilled water (蒸馏⽔) 1000ml
Agar (琼脂) 20g pH
适⽤范围：红霉素链霉菌
28. Yeast Extract Peptone （酵母膏、蛋⽩胨琼脂）资产负债表的编制
