f/2 Medium
(Guillard & Ryther 1962, Guillard 1975)
To 950 mL filtered awater add:
Make final volume up to 1 L with filtered awater and autoclave.
*Note: Autoclaved f/2 medium produces extensive silica precipitate. We delete silicate when it is not required by the alga (e f/2-Si medium below).
f/2 Trace Metal Solution
(Guillard & Ryther 1962, Guillard 1975)
To 950 mL dH2O add:
Make final volume up to 1 L with dH2O. Autoclave.
f/2 Vitamin Solution
(Guillard & Ryther 1962, Guillard 1975)
To 950 mL dH2O add:
Make final volume up to 1 L with dH2O. Autoclave and store in refrigerator. Note: Vitamin B12 and biotin are obtained in a crystalline form. When preparing the vitamin B12stock solution, allow for approximately 11% water of crystallization (for each 1 mg of Vitamin B12, add 0.89 mL dH2O). When preparing the biotin stock solution, allow for approximately 4% water of crystallization (for each 1 mg of biotin, add 9.6 mL dH2O).
f/2 Derivatives
Black Sea Medium: For brackish water organisms (16 psu, half-strength nutrients). Combine 500 mL f/2 medium and 500 mL dH2O. Autoclave.参与者英文
royal什么意思
f/2 agar: Prepare 1 liter of f/2 medium and dissolve 9g Bacto-agar (heat and mix). For test tubes, dispen dissolved agar medium into tubes, autoclave, and then cool with tubes slanted at an angle. For Petri plates, autoclave in a flask, cool almost to the gelling point, and then aptically dispen into sterile Petri plates. Note: Agar can be added to other media (e.g., f/50 agar), and agar concentration can be varied to produce softer or firmer substrates.
杨桃的英文
f/2-Si: Prepare as for f/2 medium but omit Na2SiO3 · 9H2O. This is preferred over f/2 medium for organisms with no silica requirement becau less precipitation forms.
f/2 + Se: Extra silicon and lenium are beneficial to veral diatom strains. Prepare 1 L of f/2 medium but u 2 mL of silicate stock, then add 1.0 mL of lenium stock solution (1.29 mg H2SeO 3 /L distilled H2O). Autoclave.
f/2 (11 psu): For brackish water organisms. Mix 650 mL distilled H2O and 350 mL filtered awater. Add f/2 medium nutrients and autoclave.fidel castro
加拿大留学优势
f/2-Si (24 psu): Mix 750 mL distilled H2O and 250 mL filtered awater. Prepare as for f/2 medium but omit Na2SiO 3 · 9H2O.
eddie怎么读f/4: Add 500 mL f/2 medium to 500 mL filtered awater, then autoclave.
sick什么意思
f/4-Si: Autoclave 1 L of filtered awater. When cool, aptically add f/2-Si nutrients at half concentration (i.e., 0.5 mL).
considerably>coldturkeyf/20-Si: Autoclave 1 L of filtered awater. When cool, aptically add f/2-Si nutrients a t one tenth concentration (i.e., 100 μL).
f/50-Si: This is more than a 1/25 dilution of f/2-Si medium. We autoclave 1 L of awater in a Teflon-lined bottle. Wait for the autoclaved awater to cool to room temperature (important). Aptically add 40 μL of sterile f/2 nutrients (20 μL of vitamins).
f/50-Si + CCMP1320 as food:Prepare f/50 and aptically add 50 μL of healthy, moderately den culture of CCMP1320.
f/2m: To 1L f/2 medium add 1 g methylamine · HCl, mix until dissolved and autoclave. This medium is ud to test for contamination by methylaminotrophic bacteria.
f/2p: To 1 L f/2 medium, add 1 g Bacto-peptone, mix until dissolves and autoclave. This medium is ud to test for contamination by non- methylaminotrophic bacteria and fungi.
f/2pm: To 1L f/2 medium add 1 g Bacto-peptone and 1 g methylamine · HCl, mix until dissolved and
autoclave. This general medium is ud to test for contamination by bacteria and fungi.
f/2 + NPM: Add f/2 nutrients to 900 mL of awater and autoclave. After cooling, aptically add 100 mL of the following organic stock solution. Dispen aptically into test tubes.
Organics Stock Solution
烟熏眼妆(modified from Guillard 1960)
To 900 mL dH2O add:
Bring up to 1 L with dH2O. Dispen in small aliquots and autoclave.
References
Guillard, R.R.L. 1960. A mutant of Chlamydomonas moewusii lacking contractile vacuoles. J. Protozool. 7: 262-268.
Guillard, R.R.L. 1975. Culture of phytoplankton for feeding marine invertebrates. pp 26-60. In Smith, W.L. and Chanle,y M.H. (eds.) Culture of Marine Invertebrate Animals. Plenum Press, New York, USA.
Guillard, R.R.L. and Ryther, J.H. 1962. Studies of marine planktonic diatoms. I. Cyclotella nana Hustedt and Detonula confervacea Cleve. Can. J. Microbiol.8: 229-239.
