学校代码：10564 学号：2016307373
分类号：S859.7 密级：
硕士学位论文
芹菜素-7-O-葡萄糖苷体外抗氧化及
抗炎活性研究
岳茹凤
汤有志副教授
第一指导教师：
母亲节快乐英文怎么写
第二指导教师：
学院名称：
兽医学院
兽医硕士
专业学位类：
无
领域：
答辩委员会主席：刘雅红教授
中国·广州
2018 年6 月
华南农业大学
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摘要
氧化应激是指动物体内氧化与抗氧化作用失衡，自由基长期过量产生后在动物体内产生的一种负面作用，被认为是导致动物衰老、疾病的重要因素之一。动物养殖过程中，氧化应激可引起的动物疾病有心脏病、肾脏病、腹水症、围产期疾病、胎衣不下、乳房炎、消化道炎症等，进而影响动物生产，威胁养殖业健康发展。氧化应激还可诱导环氧合酶（Cyclooxygena，COX）和脂氧合酶（Lipoxygena，LOX）的表达，促进炎症介质的分泌，引起动物机体的炎症反应，降低动物生产性能。芹菜素-7-O-葡萄糖苷（Apigenin-7-O-glucoside，AG）为洋甘菊水提物中主要的活性黄酮类化合物，AG被认为是其最丰富的成分之一，具有抗氧化和抗炎活性，其抗氧化及抗炎活性作用尚未被完全阐明。本文进行了AG抗氧化、抗炎活性的初步研究，以期为AG在兽医临床应用提供依据。
本论文通过1,1-二苯基－2-三硝基苯肼自由基（1,1-diphenyl-2-picrylhy-drazyl , DPPH）法及铁离子还
原/抗氧化能力法（Ferricion reducing antioxidant power, FRAP），对AG在化学体系中抗氧化能力进行初步评价，发现AG捕获DPPH自由基及Fe3+还原能力较弱；通过2,2-偶氮二（2-甲基丙基咪）二盐酸盐（2,2-azobis （2-amidinopropane）hydrochloride, AAPH）诱导绵羊血红细胞、质粒pBR322氧化损伤，Cu2+/H2O2诱导牛血清白蛋白（Bovine rum albumin, BSA）氧化损伤，研究AG在化学模拟生物体系中的抗氧化能力，结果发现AG在上述体系中能很好的减轻绵羊血红细胞、质粒pBR322及BSA受自由基氧化损伤程度，表现出较好的抗氧化活性。泥淖
本论文以脂多糖（Lipopolysaccharide，LPS）刺激RAW264.7细胞，构建炎症模型，对AG抗炎活性进行评价。发现AG能明显减少RAW264.7细胞中一氧化氮（Nitric oxide，NO）产生，减少细胞肿瘤坏死因子（Tumour necrosis factor-α，TNF-α）、白介素6（Interleukin-6，IL-6）的分泌，通过逆转录聚合酶链式反应（Rever transcripta-polymera chain reaction，RT-PCR）研究发现AG还能抑制经LPS诱导RAW264.7引起细胞内TNF-α、IL-6及一氧化氮合酶（Inducible nitric oxide syntha，iNOS）mRNA 的表达。表明AG能很好的缓解LPS刺激RAW264.7细胞产生的炎症反应，在该体系中具有良好的抗炎活性。
关键词：氧化应激；黄酮；芹菜素-7-O-葡萄糖苷；抗氧化；抗炎
Rearch on in Vitro Antioxidant and Anti-inflammatory Activities of
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Apigenin-7-O-glucoside
Yue Rufeng
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(College of Veterinary Medicine, South China Agricultural University, Guanzhou 510642,buildup
P. R. China)piggybank
Abstract
Oxidative stress refers to the imbalance of oxidation and antioxidation in animals. It is a negative effect of the long-term overproduction of free radicals in animals. It is considered to be one of the important factors leading to animal aging and dia. During animal breeding, oxidative stress can cau animal dias such as heart dia, kidney dia, ascites, perinatal dias, retention of placenta, mastitis, and digestive tract inflammation, which in turn affect animal production and threaten the healthy development of the aquaculture industry. Oxidative stress also induces the expression of cyclooxygena (COX) and lipoxygena (LOX), promotes the cretion of inflammatory mediators, induces inflammatory reactions in animals and reduces animal performance. Apigenin-7-O-glucoside (AG) is the main active flavonoid in the water extract of Cham
omile. AG is considered to be one of its most abundant components, and it has antioxidant and anti-oxidant properties. The inflammatory activity, its antioxidative and anti-inflammatory activity has not yet been fully elucidated. This article has carried on the preliminary study of AG anti-oxidation, anti-inflammatory activity, in order to provide the basis for AG in the veterinary clinical application.
In this thesis, 1,1-diphenyl-2-picrylhy-drazyl (DPPH) and ferricion reducing antioxidant power were ud. (FRAP), preliminary evaluation of the antioxidant capacity of AG in the chemical system, found that AG capture DPPH free radicals and Fe3+ reducing ability is weak; through 2,2'-azobis (2-amidinopropane) hydrochloride (AAPH) induces oxid ative damage of red blood cells and plasmid pBR322, and Cu2+/H2O2 induces oxidative damage of bovine rum albumin (BSA). Antioxidant capacity in the biological system, the results found that AG in the above system can be a good reduction of red blood cells in sheep, plasmid pBR322 and BSA free radical oxidation damage, showing good antioxidant activity.
In this thesis, RAW264.7 cells were stimulated with lipopolysaccharide (LPS) to construct an inflammatory model, and the anti-inflammatory activity of AG was evaluated. It was found that AG can significantly reduce the production of nitric oxide (NO) in RAW264.7 cells and reduce the tumor necrosis factor-α (TNF-α) and interleuk in-6 (IL-6). cretion. Rever transcripta-polymera chai
n reaction (RT-PCR) studies revealed that AG can also inhibit RAW264.7 cell mRNA expression of intracellular of inducible Nitric oxide syntha (iNOS), TNF-α, and IL-6 by LPS-induced through rever transcripta-polymera chain reaction. It was shown that AG can well alleviate the inflammatory respon produced by LPS-stimulated RAW264.7 cells and has good anti-inflammatory activity in this system.shoeplay
Key words: oxidative stress; flavonoids; apigenin-7-O-glucoside; antioxidant; anti- inflammatory
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