机能实验报告 humor
Ach对离体家兔小肠运动作用
client version 摘要
目的:观察乙酰胆碱对离体家兔小肠肠肌的影响和机制。方法:将离体小肠固定在离体小肠灌流装置里,在保证各影响因素不相互干扰的情况下,分别给予家兔离体肠肌标本生理盐水(Nacl)、Ach、阿托品、阿托品+乙酰胆碱刺激,完成每次刺激并出现明显现象后用38℃的台氏液冲洗肠肌标本,观察其收缩活动的特点并记录。结果:结果显示当滴加生理盐水(Nacl)刺激离体肠肌时,张力曲线没什么变化;当用乙酰胆碱刺激离体肠肌时,张力曲线则明显上升,且频率加快;当滴加阿托品刺激时,张力曲线明显下降,且频率减慢;当滴加乙酰胆碱加上阿托品刺激时,张力曲线变化较不规则。结论:乙酰胆碱(Ach)对离体家兔肠肌有增强其运动的作用,且作用于M受体。 中文关键词:离体家兔小肠肠肌;乙酰胆碱;张力曲线 Abstract:
Objective: to obrve the acetylcholine on the influence and mechanism of intestinal musc
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形容词和副词le of rabbit small intestine in vitro. Methods: in vitro small intestine in vitro intestinal perfusion device fixed, in guarantee under the condition of each influence factor does not interfere with each other, in vitro intestinal muscle specimens were given the rabbit saline (Nacl), Ach, atropine, atropine + acetylcholine stimulation, complete with 38 ℃ after each stimulus and a significant phenomenon of Taiwans fluid colonics muscle specimens and obrve the characteristics of its contraction activities and record. Results: the results showed that when add saline (Nacl) stimulation in vitro intestinal muscle, nothing change tension curve; When using the acetylcholine stimulation in vitro intestinal muscle, tension curves are obvious ri, and the frequency to speed up; When add atropine stimulation, tension curve decread obviously, and slow frequency; When add the acetylcholine and atropine on, tension curve is irregular. Conclusion: acetylcholine (Ach) on in vitro rabbit intestinal muscle has enhanced the role of the movement, and the effects on M receptor.
unknow [Key words]:Isolated rabbit intestinal muscle;Acetyl choline;Tension curve.松鼠英文
引言
吸血鬼日记 第二季
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消化道平滑肌与骨骼肌、心肌一样,具有肌肉组织共有的特性,如兴奋性、传导性和收缩性等。但消化道平滑肌兴奋性较低,收缩缓慢,富有伸展性,具有紧张性、自动节律性,对化学、温度和机械牵张刺激较敏感等特点。给予离体肠肌以接近与在体情况的适宜环境,消化道平滑肌仍可保持良好的生理特性。Ach能激动M受体,而阿托品能阻断M受体。
1.材料和方法
1,1材料:家兔、HSS-1(B)型恒温浴槽、台氏溶液、生理盐水、Ach、阿托品、通气机、超级恒温器、张力换能器、生物信号采集处理系统 ;
1.2方法:(1)实验装置准备和仪器参数设置:把台式液在恒温器中加热并保持到38℃左右,然后在麦氏浴槽中加入一定量的38℃左右的台式液。通气管接95%O2+5%CO2混合气体。用螺丝夹调节气体管道的气体流量,调节至浴槽中气体一个个逸出为止。换能器输出线接微机生物信号处理系统,设置好相关仪器参数;
在职研究生有双证吗英语六级试卷 (2)离体家兔十二指肠标本制作:取家兔一只,用木槌击其头部至晕,立即剖开腹腔,找到
十二指肠,取出十二指肠,置于冰冷的氧饱和的台氏液培养皿中,沿肠壁除去肠系膜,用5ml注射器取台氏液将肠内容物冲洗干净,然后将十二指肠剪成1-2cm数小段,换以新鲜,通入95%氧气和5%二氧化碳的混合气体的台氏液备用。把十二指肠管置于有台氏液的培养皿中,然后穿线结扎2cmz左右肠肌的两端,然后把通混合气体的机制放在浴槽中一会;
(3)标本固定:肠管一端连线系于浴槽固定钩上,然后放入38℃HSS-1(B)型恒温浴槽中,再将肠管另一端系结在张力换能器的悬梁壁上,调节肌张力1.87g,在其中加入20ml的38℃的台氏液; (4)实验观察:离体肠管在浴槽中稳定适当时间后,记录一段正常张力曲线。然后滴加0.2ml的生理盐水,观察并记录其曲线张力,反应稳定换液冲洗,以此方法依次做如表1.的添加实验;