• 1750 •广东医学2020 年 9 月第 41 卷第17 期Guangdong Medica丨Journa丨Sep. 2020, Vol. 41，No. 17
地塞米松对小鼠急性胰腺炎中miRNA - 155及
菠萝的英文NF- k B信号通路的影响*
陈希妍，杨飞云，杨亚琴，牛丽丹，马彦娟，石金河，郝同琴&
新乡医学院第一附属医院急诊科（河南卫辉453100)
【摘要】目的探讨地塞米松（dexam ethasone,DEX)对急性胰腺炎（acute pancreatitis，AP)小鼠模型胰腺
组织中m iR N A- 155及核因子-k B(N F- k B)信号通路的影响。方法利用蛙皮素建立小鼠AP动物模型，
实验分组为对照组、模型组、地塞米松组（DEX组），分别连续3次予生理盐水、蛙皮素（10 (xg/iriL浓度按50 pVkg)、蛙皮素（剂量同模型组）+DEX(1 m L/kg)腹腔注射。利用ELISA法检测各组动物胰腺组
织及血清中
肿瘤坏死因子-a(tu m o r necrosis factor- a，T N F- e x)、白细胞介素-6 (interleukin- 6，IL- 6 )、白细胞介素-
1P(interleukin- 1p，IL- 1p)的含量；利用实时荧光定量P C R(R T- qPC R)检测各组动物胰腺组织中m iR N A-
155 niRNA的表达情况；利用Western丨)lot检测各组胰腺组织中嶙酸化p65及丨k B c x蛋白的表达情况结果
widebandELISA检测结果显示，与模型组比较，DEX组动物胰腺组织及血清中IL- 1p、T N F- a与IL- 6的含量显著降
低，对照组最低。RT-qPCR结果显示，与模型组相比较，DEX组胰腺组织中miRNA-155 mRNA的表达显著
regulation降低，对照组最低。W estern blot结果显示，与模型组相比较，DEX组胰腺组织中磷酸化p65及I k B c x蛋白的表
达均显著降低。结论 DEX可能通过抑制m iR M A-155的表达，进而抑制p65及I k B c x蛋白的磷酸化，从而抑
制N F- K B信号通路的活化，减少炎症因子如IL- 1p、T N F- a与IL- 6等的合成与释放，对胰腺腺泡细胞起
jaci velasquez destiny
到保护作用。
【关键词】急性胰腺炎；miRNA-155; NF-k B信号通路；地塞米松
【中图分类号】R576;R459.7 【文献标志码】A
DOI：10.13820/jki.gdyx.20200342
lhdThe effect of dexamethasone on the miRNA - 155 and NF -k B signaling pathway in mice with acute pancreatitis.
CHEN Xi -y a n^YANG Fei - yun, YANG Ya - q in, N1U Li -dan, MA Yan - jua n, SHI Jin - h e, HAO Tong - qin. Department o f Emergency, the First Affiliated Hospital o f Xinxiang Medical University, Weihui 453 \00, Henan, China Corresponding author:HAO Tong - qin. E -mail:****************
【Abstract] Objective Fo investigate the effect of dexamethasone on the miRNA -155 and NF -k B signal path
way in pancreas in mice with acute pancreatitis (A P). Methods Establishm ent of the AP mou model by using caer-
ulein. The mice were randomly divided into control group (th e saline group) , the model group and dexamethasone group
(DEX g ro u p). The contents of TNF -a and IL - 6, IL -1 p in pancreas tissue and rum in each grouj) were tested using
plankingELISA method. RT - qPCR was ud to asss expression of miRNA -155 mRNA in pancreas. W estern blot was ud to
asss expression of p - p65and p -I k B«proteins in pancreas. Results The results of ELISA test showed that dexametha
sone could significantly reduce the contents of IL -1 p, TNF -a and IL -6in pancreas tissue and rum, comparing with
power是什么意思
圣诞快乐 英语the model group. The RT - qPCR results showed that dexamethasone could significantly reduce the expression of miRNA -
155 mRNA in pancreas tissue, compared with the model group. Western blot results showed that dexamethasone could sig
nificantly reduce the expression of p -[^65 and p -iKBa proteins in pancreas tissues, compared with the model group. Conclusion Dexamethasone could inhibit the expression of miRNA -155 , further inhibit the phosphorylation of p65 and
I k B cx，thus inhibit the activation of NF —k B signaling pathway. The reduce the synthesis and relea of inflammatory
factors such as IL —1 p, TNF — a and IL -6.It plays protective effect on pancreatic acinar cells.
英文字母顺序
【Key words】acute pancreatitis;miRNA-155 ;NF-k B signaling pathway;dexamethasone
好文章阅读网*基金项目：河南省组织再生重点实验室开放课题项目（KFKT16003)
A迪fg作者：郝同琴，主任医师；E - mai丨:****************
