目录
缩略词表 (1)
英文摘要 (2)
中文摘要 (4)
论文正文AhR活化上调肠上皮源IL-10对DSS诱导的小鼠结肠炎的保护作用及机制研究 (6)
第一章前言 (6)
第二章FICZ对DSS诱导的小鼠结肠炎的缓解作用 (8)
2.1 实验材料与方法 (8)
2.2 实验结果 (11)
2.3 讨论 (13)
第三章活化的AhR上调肠上皮源IL-10的表达并抑制炎性因子的表达 (15)
3.1 实验材料与方法 (15)
3.2 实验结果 (24)
3.3 讨论 (28)
第四章活化的AhR通过上调P-STAT3调控IL-10的表达 (29)
4.1 实验材料与方法 (29)
4.2 实验结果 (33)
4.3 讨论 (37)
全文结论 (38)
参考文献 (39)
alphabetsong文献综述白介素10与炎症性肠病 (44)
参考文献 (49)
读硕士学位期间发表论文情况 (57)
致谢 (58)
缩略词表
leapmotion英文缩写英文全称中文全称
AhR Aryl hydrocarbon receptor 芳香烃受体
FICZ 6-formylindolo[3,2-b]carbazole 6-甲酰基吲哚并[3,2b]-咔唑
IBD TCDD Inflammatory bowel dia
Tetrachlorodibenzo-p-dioxin
炎症性肠病
四氯二苯并-p-二恶英
ILC Intestinal epithelial cell 肠道上皮细胞IL-10 Interleukin-10 白介素10
aluminumalloy
festivals是什么意思IL-1β Interleukin-1β白介素1β
TNF-αHE Tumor necrosis factor-α
Hematoxylin-eosin
肿瘤坏死因子α
苏木精-伊红
IL-6 Interleukin-6 白介素6i still
CYP1A1 CytochromeP450,family1,subfamilyA,
polypeptide1
getout
细胞色素P450 1A1 GI Gastrointestinal 胃肠的
DSS Dextran sulfate sodium 葡萄糖硫酸钠经典英文台词
LPS Lipopolysaccharides 脂多糖
TBST PBS Tris buffered saline tween
Phosphate buffer solution
洗膜缓冲液
原理图英文磷酸盐缓冲液
BSA Bovine rum albumin 牛血清白蛋白
WB PCR Western blot
Polymera chain reaction
蛋白免疫印迹
聚合酶链式反应
RT-PCR Real-Time
PCR 荧光定量PCR
RNA DNA DMSO Ribo Nucleic Acid
Deoxyribonucleic acid
Dimethyl sulfoxide
核糖核酸
robert holley
脱氧核糖核酸
二甲基亚砜
STAT3 Signal transducer and activator of
transcription 3
信号传导与转录激活因子3
shij
Activation of Aryl Hydrocarbon Receptor Up-regulates Epithelial cells derived IL-10 expression and Meliorates Dextran Sulfate Sodium-Induced Murine Colitis
Abstract
Background:
Inflammatory bowel dia (IBD), consisting of ulcerative colitis and Crohn’s dia，is a major chronic disorder that affects the GI tract of humans. Although the preci pathogenesis of colitis is inadequately understood, prent evidence indicates that is caud by dysregulated mucosal immune respon and epithelial barrier dysfunction.
The aryl hydrocarbon receptor (AhR) is a cytoplasmic transcription factor activated by a large variety of environmental agents. Activation of the AhR is involved in the control of intestinal mucosal homeostasis. Evidences have suggested that AhR is suppresd in the intestines of IBD patients, and activation of AhR by ligands such as FICZ or TCDD inhibits the inflammatory respon in murine colitis models. Although a number of studies have suggested a protective role for AhR in colitis, the preci underlying mechanism remains unclear.
Interleukin-10 (IL-10) is an important anti-inflammatory cytokine that ameliorates mucosal inflammation. The goal of this study was to investigate the role of AhR activation in alleviating intestinal inflammation by upregulating IL-10 in intestinal epithelial cells (IECs).
Methods:
1. Colitis was induced in mice by the administration of 4% dextran sulphate sodium (DSS) for 7 days. The mice were given injections of the AhR agonist 6- formylindolo (3,2-b)carbazole (FICZ) starting 2 days after the first administration of DSS. Mice were weighted, colon tissues were collected and measured, and histology analys were performed.
2. IECs were isolated from colon, and the expression of IL-10 in IECs was assd by Western blot analysis. The mRNA levels of inflammatory cytokines were assd by
