samsonite甘蔗蜡提取物多廿烷醇的降血脂机制研究nope
作者:廖芳,陈伟伟
来源:《湖北农业科学》2013年第05期
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摘要:采用RT-PCR、Western Blot、L-[35S]放射性标记物掺入等方法,观察甘蔗蜡提取物多廿烷醇对Hep G2细胞羟甲基戊二酸辅酶A(HMG-CoA)还原酶mRNA转录及蛋白质表达量的影响,及其对HMG-CoA还原酶合成和降解的作用。结果表明,多廿烷醇降低HMG-CoA还原酶mRNA的转录和蛋白质的表达量,且作用效果随多廿烷醇浓度的升高而增强。此外,多廿烷醇抑制HMG-CoA还原酶的合成并加速其降解,多廿烷醇处理后HMG-CoA还原酶的半衰期明显缩短。
spaghetti 关键词:多廿烷醇;Hep G2细胞;羟甲基戊二酸辅酶A(HMG-CoA)还原酶
中图分类号:R285.5 文献标识码:A 文章编号:0439-8114(2013)05-1125-03
Study on the Hypolipidemic Mechanism of Policosanol Extracted from Sugarcane Wax
元旦快乐英语 LIAO Fang,CHEN Wei-wei无所谓的英文
inui (Department of Pharmacology, Gannan Medical University, Ganzhou 341000, Jiangxi, China)
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电脑游戏的危害 Abstract: The effects of policosanol extracted from sugarcane wax on mRNA transcription and protein expression level, synthesis and degeneration of 3-hydroxy-3-methylglutarl-coenzyme (HMG-CoA) reducta in Hep G2 cell were determined by RT-PCR, Western Blot and L-[35S] radioactive isotope tracing technology. The results showed that the mRNA transcription and protein expression level of HMG-CoA reducta decread with the increa of policosanol concentration(10,20,30 μg/L). Policosanol also suppresd the synthesis of HMG-CoA reductas while sped up its degeneration; the half life of HMG-CoA reductas treated by policosanol was shortened obviously.
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