收稿日期:2007-07-15
基金项目：国家自然科学基金项目(30360060)；海南
省自然科学基金项目
作者简介王 英()，女，陕西长安人，博
士，华南热带农业大学讲师3通讯作者甘蔗种质遗传基础的ISSR 分析
王 英，庄南生3，高和琼，黄东益
(华南热带农业大学农学院，海南儋州 571737)
英语二历年真题摘 要：采用ISSR 分子标记技术对96份甘蔗种质(39份祖亲种、57份栽培品种或品系)的遗传基础进行了分析.利用筛选出的7对多态性较强的引物，构建了甘蔗96份种质的ISSR 指纹图谱，这7对引物组合共扩增出85条谱带，其多态性为100%.96份种质的遗传相似系数变化范围在01459～01918，平均01656.聚类分析表明，随着相似系数结合线的不同，可分别将参试的甘蔗种质从属间(甘蔗属与斑茅种)、野生种(割手密种、大茎野生种、印度种、中国种)与栽培种(热带种)间、栽培种与杂交栽培品种(或品系)间区别开来.各祖亲种与杂交栽培品种(或品系)的遗传相似性由大到小依次为热带种、印度种和中国种、大茎
野生种、云南割手密种，其他割手密种，斑茅.多数品种(品系)的ISSR 聚类结果与系谱基本相符，某一品种常与其父代或其祖代的某一亲本聚在同一组中.
关 键 词：甘蔗；种质资源;invalidate>fec
ISSR ；遗传基础中图分类号:S566文献标识码:A
ISS R analysis f or sugarcane ger mpla smlove of iris>单片机培训
WANG Ying ,ZHU ANG N an 2sheng 3,GA O H e 2qong ,HU ANG Dong 2yi
车挂件(C ollege of Agronomy ,S outh China Univers ity of T ropical Agriculture ,D anzhou ,H ainan 571737,China)
wengoAbstra ct :Modern sugarcane cultivars (S acchar um spp 1)are polyploid or aneu ploid clones wit h complex genetic background,a nd t he major components of the genome derived f rom S 1of ficina rum 1and the remainder f rom S 1spontaneum ,S 1ba rberi ,S 1robustum ,S 1sinen a nd Eria nthus a rundinaceus 1In this study ,96accessi ons of sugarcane germplasms (39parental s pecies ,57cultivars or clones)were a nalyzed by Inter 2Simple Sequence Repeat s (ISSR ).7primer s were lected and ud to esta b lish the ISSR fin gerprints of the germplasms 1There were 85polymorphi
拉姆斯 波顿
c ba nds a nd were accounting for 100%1The ISSR data were clustered us ing UP G MA method 1The genetic similarit y coefficients among 96ger mplasms ranged f rom 01459to 01918with a mea n of 016561Bad on the differe nce of the similarity coefficients ,the sugarcane germplasms could be distinguished among intergenus (Saccha rum and E 1ar undinaceus ),wild species (S 1spontaneum ,S 1bar b eri ,S 1sinen a nd S 1robustum ),cultivated s pecies (S 1off icina rum )1cultivated s pecies a nd cultivars derived from inters pecific hybridization 1The similarity between cultivars and parental s pecies S 1off icinar um were the highest ,a nd followed by S 1bar b eri ,S 1si nen ,S 1robustum ,S 1spontaneum and E 1a rundinaceu s in the or der from high to low 1Clustering bad on ISSR sh owed t hat the grou pings of most cultivar s were corres ponded to their pedigrees in which a given cultivar and one of its parents was always clustered into t he same grou p 1
K ey w or ds :suga rcane ;ger mplasm ;ISSR ;ge netic ba
甘蔗是世界上主要的糖料作物之一，其遗传
背景非常复杂.甘蔗属(Saccha ru m )内分为热带种(S 1of fici n aru m )、印度种(S 1barberi )、中国种(S 1)、割手密种(S 1)和大茎野生种(S 1f )等多个原始野生种[]第33卷
2007年8月湖南农业大学学报(自然科学版)Jour nal of Huna n Agricultural Univer s ity (Natural Scienc
hot stuffes )Vol 133Aug 12007霜牙
8:1971-.
.sinen s pon ta neu m robus u m 1.
