认证培训2020年33卷6期Vol.33No.6
!"农业学& SouthwestChinaJouenaaoeAgeicuatueaaSciences1327
文章编号：1001-4829(2020)6-1327-05DOI：10.ki.scjvs.2020.6.035罗氏沼虾TRQ基因生物信息学及其mRNA表达分析姜建萍1>2，袁翔1，邱庆庆蔦黄光华3，杨秀荣1，蒋和生1，杨学明3*，蒋钦杨1foolproof
(1•广西大学动物科学技术学院，广西南宁5300042广西药用植物园，广西南宁530023；3•广西水产科学研究院，广西南宁
在线朗读>夏天的英文单词530021)
摘要：【目的】分析罗氏沼虾(Macroorachium ronbergii)胰蛋白酶(TRY)基因的生物信息学及其mRNA的表达情况，为深入探究TRQ基因的生物学功能和提高罗氏沼虾产量提供参考依据&【方法】利用MevA/gn、PRtPwvm、PmtScWe和MLRC等在线软件对罗氏沼虾FRQ基因进行生物信息学分析，采用实时荧光定量PCR检测其在罗氏沼虾腹部神经、胃、心脏、R、性腺、肝胰腺、肌肉和肠道等8个组织中的表达情况，进一步分析TRQ基因在罗氏沼虾生长快速家系(FG)和生长慢速家系(SG)个体肌肉组织中的表达规律&【结果】罗氏沼虾TRY氨基酸序列与刀额新对虾#Metapeeaeue enii TRY氨基酸序列的同源性
最高，与凡纳滨对虾(Litopenaee ◎annamei)TRY氨基酸序列的同源性最低&罗氏沼虾TRY蛋白的分子量为28.54kD%理论等电点为4.44，属于疏水性蛋白&罗氏沼虾TRY蛋白二级结构中)螺旋占17.67%%延伸链占21.05%%无规则卷曲占61.28%&罗氏沼虾TRQ基因在所检组织中均有表达，在雌性和雄性个体的肝胰腺组织中均高表达，而在雌性个体的R和雄性个体的肌肉组织中低表达&此外，罗氏沼虾TRY基因在FG个体肌肉组织中的表达量极显著高于在SG个体肌肉组织中的表达量#P<0.01)&【结论】罗氏沼虾与刀额新对虾的亲缘关系最近;TRY基因在罗氏沼虾各组织中均广泛表达％以在雌、雄罗氏沼虾肝胰腺组织中表达量最高，且存在性别差异性&TRY基因在罗氏沼虾生长发育过程中发挥了重要作用，可能正向调控罗氏沼虾的生长发育&
可爱用英语怎么说
关键词：罗氏沼虾'生长性状；FRY基因'基因表达'生物信息学
stv中图分类号:S917.4文献标识码：A
Bioinformatice Analysie and mRNA Expression of
TRY Geee in Macrobracyium ronbergi i
JIANG Jian-ping1,2&YUAN Xiang1,QIO Qing-qing1&HUANG Guang-hua3&YANG Xiu-rong1,
JIANG He-sheng1&YANG Xue-ming3*&JIANG Qin-xang1*
中国梦演讲稿教师篇(1-Col b ye of Animal Science and Technology,Guanyvi Univevip,Guanyvi Nanning530004,China；2-Guanyvi Botnical Garden of Medicinal Plants,Guanyvi Nanning530023,China；3-Guanyvi Academy of Fisheg Sciences,Guanyvi Nanning530021,China)
Abstract:【Objective]The purpo of this study was A undevtand the biological function and increasing the production of Macrobrachium ronbergii by analyzing bioinformatics of tgpsasc(TRY)gene mRNA expression pWtern.【Method]Softpares including MeyA/gn,Prot-Paeam,PeotScaaeand MLRCweeeud topeeeoem the bioin eo ematics ana aysis,and eeaa-timeeauoeescencequantitatieePCR(RT-PCR)was ud todetecttheeipee s ion oe TRY gene among the abdomina agang aion,stomach,hea et,gi a,gonad,hepatopanc eeas,musc ae and intestine, and furPer explored its expression in the muscle of individuals from fasC5mwing families and slow-5rowing families.【Result]The homology of amino acib quence of TRY gene bergi i was the highest and lowest compared with the amino acid quences of Metapenaeus ensis and Liopenaee eannamei,respectively.The molecular weight of TRY protein was28-54kD,and the theoretical pl was4.44,belonging A the hydmphobic protein.The condag st/cturo of coding protein was consisted of)-helix,extended strand and random coil,accounting for17.clipper
67%,21.05%and61.28%,respectively.-RY gene was widely expresd in the tested tissues,and was
highly expresd in the hepatopan-
收稿日期：2020-01-02
基金项目：广西科技重大专项(桂科AA17204080-6"'广西自然科学基金项目(2018GXNSFBA281209)'广西博士后“两站”项目(T3340097968)fit
英语短故事
作者简介：姜建萍(1988-)&女，重庆开州人，博士，主要从事动物遗传育种研究工作，E-mail：jianbmnping818@126-com；*为通讯作者：杨学明(1969-),E-mail:nnyxming@126-com；蒋钦杨(1981-),E-mail：jiangqinyang@126-com。cmas of females and males.Meanwhile,it had a lower expression in gill of females and in muscle of males.In addition,themRNA eipee s ionsoe TRY genein east-geowing geoup(FG)wassignieicantayhigheethan thatin saow-geowing group(SG)( P<0.01).【Conclusion] M.conbergii had the caosteeaationship with M8eneie*TRY genewaswideayei-presd in the tissues bergii,but it was shown the highe2teipee2ion aeeeain hepatopanceea2,and thediteibution oe TRY wa2di e eentbetween eemaae2and maae2.Thu2,TRY
