MCF-7 Cells
human breast adenocarcinoma cell line
细胞名称 | MCF7 |
种属 | 人 |
组织来源 | 乳腺腺癌 miracle是什么意思 |
形态特点 | 上皮细胞 |
致瘤性 | + |
产物或抗原 | 产生胰岛素样生长因子结合蛋白（IGFBP）BP-2，BP-4，BP-5；血型抗原O，Rh+；月份 英文 |
受体 | 雌激素受体+ |
癌基因 | Wnt7h+ |
prospects同工酶 | PGM3, 1; PGM1, 1-2; ES-D, 1-2; AK-1, 1; GLO-1, 1-2; G6PD,B |
染色体核型 | The stemline chromosome numbers ranged from hypertriploidy to hypotetraploidy, with the 2S component occurring at 1%. There were 29 to 34 marker chromosomes per S metapha; 24 to 28 markers occurred in at least 30% of cells, and generally one large submetacentric (M1) and 3 large subtelocentric (M2, M3, and M4) markers were recognizable in over 80% of metaphas. No DM were detected. Chromosome 20 was nullisomic and X was disomic. |
生长特性 | 粘附 |
文献评价奥巴马竞选总统演讲 | MCF7细胞系表现出分化的乳腺上皮细胞的一些特征，包括通过胞浆雌激素受体呈递雌二醇和形成dome等。MCF7细胞系包含Tx-4癌基因。肿瘤坏死因子α（TNFα）抑制MCF7细胞系的生长。抗雌激素处理可以调节IGFBP的分泌；MCF7细胞系caspa3基因的第3外显子有47bp缺失，导致在成熟mRNA剪接过程中整个第3外显子缺失；第3外显子含有125bp，读码框架的改变使得蛋白质翻译过程提前终止，因此开始的英文MCF7细胞系不能检测到caspa3蛋白的表达；Caspa3的缺失使得MCF7细胞对各种化疗药物诱导的凋亡十分不敏感，重建caspa3可以增强MCF7细胞对化疗药物的敏感性 |
传代 | 吸去培养基，用0.25%胰酶、0.03%EDTA溶液漂洗一遍；吸去漂洗液，加入1-2ml胰酶/EDTA溶液，室温（或37℃）放置至细胞从培养瓶脱落。加入新鲜培养基，吹匀，分至新的培养瓶中。推荐使用1∶3-1∶6的比例传代； |
培养基更换 | 每周2-3次； |
冻存液 | 培养基 95%; DMSO, 5%； |
培养条件 | 长江英文ATCC培养基；MEM Eagle（2mM L-谷氨酰胺，1.5g/L碳酸氢钠，0.1 mM非必需氨基酸，1.0 mM丙酮酸钠，0.01mg/ml牛胰岛素，90%；胎牛血清，10%； |
参考文献bernard | CANCER RESEARCH 61, 348-354, January 1, 2001 |
THE JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 273, No. 16, Issue of April 17, pp. 9357-9360, 1998 |
THE JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 276, No. 4, Issue of January 26, pp. 2935-2942, 2001 |
THE JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 276, No. 14, Issue of April 6, pp. 10759-10766, 2001 |
The Journal of Cell Biology, Volume 148, Number 6, March 20, 1239-1254，2000 |
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备注 | 文献报道酚红有弱的雌激素作用，推荐MCF7细胞培养采用不含酚红的培养基；tell laura i love her |
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美国模式培养物集存库ATCC
ATCC可以提供以下类别生物标准品：一对一数学辅导细胞株（3000多种）；菌株（15000多种）；动植物病毒株（2500多种）以及重组物质等。
现已成为可信赖的活体微生物、细胞系等获得、保存和发放的国家资源中心。ATCC有29,000多种不同品系可靠的动物细胞和微生物培养体，它能满足各科学团体对可信赖品系的需要。仅1981年就有39,000多培养物发放给全世界的科学工作者，并成功地用冷冻和冷冻干燥的方法保存各种细胞品系。 ATCC是一个非官方(学会单位)无利润的公司，由各科学组织推选成员组成理事会进行管理。现有工作人员125人。参加的科学团体有：美国免疫协会、美国生物化学会、美国细胞生物学会、美国微生物学会、美国动物学会、美国遗传学会、原生动物学会和组织培养协会等16个学术组织。在ATCC可利用各种材料以及工具和设备进行广泛的研究。主要研究项目有：酶联免疫吸附分析技术(ELISA)，真核细胞转录
的调控，杂交瘤、淋巴类和髓类以及其他细胞系的建立，人突变细胞系的基因蛋白质，借助计算机进行鉴别分析，培养物的特征鉴别和保存技术的改进,关于化学致癌、致畸和致突变的。
The First Part
Source of the MCF-7 cell line:
MCF-7 is a cell line that was first isolated in 1970 from the breast tissue of a 69-year old Caucasian woman. Of the two mastectomies she received, the first revealed the removed tissue to be benign. Five years later, a cond operation revealed malignant adenocarcinoma in a pleural effusion from which was taken cells for MCF-7. The woman was treated for breast cancer with radiotheraphy and hormonotherapy.
Copyright picture from . Reproduced with permission from Altogen Biosystems.sinocom
Us for the MCF-7:
MCF-7 cells are uful for in vitro breast cancer studies becau the cell line has retained veral ideal characteristics particular to the mammary epithelium. The include the abili
ty for MCF-7 cells to process estrogen, in the form of estradiol, via estrogen receptors in the cell cytoplasm. This makes the MCF-7 cell line an estrogen receptor (ER) positive control cell line.
Characteristics of MCF-7:
In addition to retaining their estrogen nsitivity, MCF-7 cells are also nsitive to cytokeratin（细胞角蛋白）. They are unreceptive to desmin, endothelin, GAP, and vimentin. When grown in vitro, the cell line is capable of forming domes and the epithelial like cells grow in monolayers. Growth can be inhibited using tumor necrosis factor alpha (TNF alpha), and treatment of MCF-7 cancer cells with anti-estrogens can modulate insulin-like growth factor finding protein's, which ultimately have the effect of a reduction in cell growth.
Stability of cell line:
Genetically, the MCF-7 line has not been maintained exactly. Originally, it was described
as having a karyotype containing 85 chromosomes, which has since been reduced by 16 chromosomes. Today's cell line has a karyotype containing 69 chromosomes. Furthermore, there are genetic discrepancies between the MCF-7 cell line from the Michigan Cancer Foundation and the ATCC（美国型培养菌种集，American Type Culture Collection）cell line. This indicates that the ATCC cell line is of a different source than the other MCF-7 cell lines.
The Second Part:
MCF-7 Culturing Protocol:
1.U Eagle's MEM, supplemented with 10% FBS, 1% penicillin/streptomycin. Can also add non esntial amino acids (0.1 mM), Insulin (10ug/mL) and Sodium pyruvate (1mM). Add 10nM estrogen to media for a 3-4x increa in cell numbers. Maintain temperature at 37°C in humidified, concentrated CO2 (5%) atmosphere.
