either怎么读Cell Line Designation: U-937 ATCC® Catalog No. CRL-1593.2™ Table of Contents:
•Cell Line Description
• Biosafety Level
• U Restrictions
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•Handling Procedure for Frozen Cells
•Handling Procedure for Flask Cultures
• Medium Renewal
•Complete Growth Medium
• Cryoprotectant Medium
• References
• Replacement Policy
Cell Line Description
Organism:Homo sapiens (human)
Tissue: histiocytic lymphoma
Age: 37 years
Gender: male
Ethnicity: Caucasian
Morphology: monocyte
Growth properties: suspension
DNA profile ( STR analysis)
Amelogenin:
X
CSF1PO:
12
D13S317: 10, 12
D16S539:
12
D5S818:
12
D7S820: 9, 11
TH01: 6, 9.3
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TPOX: 8, 11
vWA:14, 15
Cellular products: lysozyme; beta-2-microglobulin (beta 2 microglobulin); tumor necrosis factor (TNF), also known as tumor necrosis factor alpha (TNF-alpha, TNF alpha), after stimulation with phorbol myristic acid (PMA)
Receptors expresd: complement (C3)
Depositors: H. Koren
Comments: The U-937 cell line was derived by Sundstrom and Nilsson in 1974 from malignant cells obtained from the pleural effusion of a patient with histiocytic lymphoma. Studies since 1979 have shown that U-937 cells can be induced to terminal monocytic differentiation by supernatants from human mixed lymphocyte cultures, phorbol esters, vitamin D3, gamma interferon, tumor necrosis factor (TNF) and, retinoic acid.
The cells are negative for immunoglobulin production and Epstein-Barr virus expression. The cells express the Fas antigen, and are nsitive to TNF and anti-Fas antibodies.
In 1994, PCR and cytogenetic analys showed that a number of stocks of U-937 were contaminated with the human myeloid leukemia cell line, K-562. In the earliest stocks available, the level of contamination was 0.6%. Distribution was discontinued in March 1994, except if required for patent purpos. Anyone who wishes to receive a sample of this original material should contact the Head of the ATCC Patent Depository.
A stock of CRL-1593™ found to be free of K-562 was propagated continuously for 8 weeks and tested weekly by PCR. Distribution and ed stocks give DNA profiles characteristic of U-937 only. Such preparations are now offered as authentic U-937 (ATCC CRL-1593.2™) and are believed to be free of cond subpopulations.
Biosafety Level: 1
Appropriate safety procedures should always be ud with
this material. Laboratory safety is discusd in the following publication: Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS Publication No. (CDC) 93-8395.
美白脸部皮肤小窍门
学韩语入门U.S. Department of Health and Human Services, Centers for Dia Control and Prevention. Washi
ngton DC: U.S. Government Printing Office; 2007. The entire text is available online at v/od/ohs/biosfty/bmbl4/bmbl4toc.htm. U Restrictions
eventfulThe cells are distributed for rearch purpos only.
The original U-937 cell line was established by Dr. K. Nilsson's laboratory in 1974 and he has requested the following: (1) In all papers reporting any u of this cell line or any derivatives thereof a direct reference should be made to Sundstrom and Nilsson (Int. J. Cancer 17: 565-577, 1976).
(2) Any propod commercial u of the cells should be negotiated with Professor Kenneth Nilsson, Rudbeck Laboratory, SE-751 85 Uppsala, Sweden. (3) No distribution of any of the cells or sublines derived therefrom should be made to third parties; (4) The cells should be ud for non-clinical, non-commercial rearch only.
Handling Procedure for Frozen Cells
To insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor pha and not at
–70°C. Storage at –70°C will result in loss of viability. SAFETY PRECAUTION: ATCC highly recommends that protective gloves and clothing always be ud and a full face mask always be worn when handling frozen vials. It is important to note that some vials leak when submerd in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas pha may result in the vesl exploding or blowing off its cap with dangerous force creating flying debris.
1. Thaw the vial by gentle agitation in a 37°C water bath.
To reduce the possibility of contamination, keep the O-
ring and cap out of the water. Thawing should be rapid
(approximately 2 minutes).
2. Remove the vial from the water bath as soon as the
contents are thawed, and decontaminate by dipping in or
spraying with 70% ethanol. All of the operations from this
point on should be carried out under strict aptic conditions.
3. Transfer the vial contents to a centrifuge tube containing
9.0 ml complete culture medium. and spin at
approximately 125 x g for 5 to7 minutes.
4. Resuspend cell pellet with the recommended complete
medium (e the specific batch information for the culture recommended dilution ratio). and dispen into a
25 cm2 or a 75 cm2 culture flask).
5. Incubate the culture at 37°C in a suitable incubator. A
5% CO2 in air atmosphere is recommended if using the
medium described on this product sheet.
Handling Procedure for Flask Cultures
The flask was eded with cells (e specific batch information), grown, and completely filled with medium at ATCC to prevent loss of cells during shipping.
1. Upon receipt visually examine the culture for
macroscopic evidence of any microbial contamination.
Using an inverted microscope (preferably equipped with pha-contrast optics), carefully check for any evidence of microbial contamination
2. Incubate the flask in an upright position for veral hours
at 37°C. After the temperature has equilibrated, aptically remove the entire contents of the flask and centrifuge at 125 xg for 5 to 10 minutes. Remove shipping medium and save for reu. Resuspend the cell pellet in 10 ml of this medium.
3. From this cell suspension remove a sample for a cell
count and viability. Adjust the cell density of the suspension to 2-3 x 105 viable cells/ml in the shipping medium.
4. Incubate the culture, horizontally, at 37°C in a 5% CO2
in air atmosphere. Maintain the cell density of the culture
as suggested under the subculture procedure. Subculturing Procedure
Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be subcultured by total medium replacement by centrifugation with subquent resuspension
at 2 x 105 viable cells/ml. Maintain cultures at a cell concentration between 1 x 105 and 2 x 106 cells/ml. Do not allow the cell concentration to exceed 2 x 106 cells/ml. Medium Renewal
Three to four times weekly Complete Growth Medium
The ba medium for this cell line is ATCC-formulated RPMI-
arletta1640 Medium, Catalog No. 30-2001.
张璐翻译To make the complete growth medium, add the following
components to the ba medium:
•f etal bovine rum to a final concentration of 10%
This medium is formulated for u with a 5% CO2 in air
atmosphere.
ATCC tested fetal bovine rum is available as ATCC
Catalog No. 30-2020 (500ml) and ATCC Catalog No. 30-
2021 (100ml).
Cryoprotectant Medium
Complete growth medium described above supplemented
with 5% (v/v) DMSO. Cell culture tested DMSO is available
as ATCC Catalog No. 4-X.
Additional Information
Additional product and technical information can be obtained
from the catalog references and the ATCC Web site at
, or by e-mail at
References
(additional references may be available in the catalog description at )
Sundstrom C and Nilsson K. Establishment and
characterization of a human histiocytic lymphoma cell
line (U-937). Int. J. Cancer 17: 565-577, 1976 PubMed:
76189427
Ralph P et al. Lysozyme synthesis by established
human and murine histiocytic lymphoma cell lines. J. Exp. Med. 143: 1528-1533, 1976 PubMed: 76192838
Koren HS et al. In vitro activation of a human
macrophage-like cell line. Nature 279: 328-331, 1979
PubMed: 79199698
Gidlund M et al. Natural killer cells kill tumour cells at
a given stage of differentiation. Nature 292: 848-850, 1981 PubMed: 81270742
Olsson I et al. Induction of differentiation of the
human histiocytic lymphoma cell line U-937 by 1
alpha,25-dihydroxycholecalciferol. Cancer Res. 43: 5862-5867, 1983 PubMed: 84055081
Morimoto H et al. Overcoming tumor necrosis factor
and drug resistance of human tumor cell lines by
combination treatment with anti-Fas antibody and drugs or toxins. Cancer Res. 53: 2591-2596, 1993 PubMed: 93265460
部分分式Giovannangeli C et al. Accessibility of nuclear DNA to
triplex-forming oligonucleotides: The integrated HIV-1
provirus as a target. Proc. Natl. Acad. Sci. USA 94: 79-84,
1997 PubMed: 97144397
Brigino E et al. Interleukin 10 is induced by
recombinant HIV-1 Nef protein involving the
calcium/calmodulin-dependent phosphodiestera
signal transduction pathway. Proc. Natl.
Reid YA et al. Cell Line Cross-contamination of U-937.
J. Leukocyte Biol. 57: 804, 1995 PubMed: 95279889
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
怨恨的意思Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
ATCC Warranty
The viability of ATCC products is warranted for 30 days from the date of shipment. If you feel there is a problem with this product, contact Technical Services by phone at 800-638-6597 or 703-365-2700 or by e-mail at Or you may contact your local distributor.
Disclaimers
This product is intended for laboratory rearch purpos only. It is not intended for u in humans.
While ATCC us reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or reprentations as to its accuracy. Citations from scientific literature and patents are provided for informational purpos only. ATCC does not warrant that such information has been confirmed to be accurate.
This product is nt with the condition that you are responsible for its safe storage, handling, and u. ATCC is not liable for any damages or injuries arising from receipt and/or u of this product. While reasonable effort is made to insure authenticity and reliability of strains on deposit, ATCC is not liable for damages arising from the misidentification or misreprentation of cultures.
Plea e the enclod Material Transfer Agreement (MTA) for further details regarding the u of this product. The MTA is also available on our Web site at
© ATCC 2010. All rights rerved.
ATCC® is a registered trademark of the American Type Culture Collection.
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