记账凭证编号邻近闪烁分析 (Scintillation Proximity Assay, SPA)mars talk
该技术主要利用能被激发冷光的特制平板(FlashPlate) 或圆球微粒 (SPA bead)，其底部或表面连接药物标的蛋白 (如受体、酶…等生物活性分子)，当药物标的蛋白与同位素标记的配体之间产生专一性的结合，在近距离释放的射线能量能激发圆球微粒上的闪烁物质惠州教育 (scintillant) 二本师范大学排名而产生冷光，相对的，不能专一性结合的分子，因无法近距离接触生活大爆炸第七季 bead，其标定的同位素所发出的射线能量大部分在水溶液消散而不足以激发产生冷光。此方法在实验过程中，不需分离步骤，且可连续侦测，常用于自动化操作与大量药物筛选
How does SPA work?
When a radioactive atom decays it releas sub-atomic particles such as electrons. The distance the particles travel through water is limited and is dependent upon the energy of the particle. Scintillation proximity assay (SPA) relies upon this limitation.
For example, when a tritium atom decays, it releas a ß-particle. If the tritium atom is within 1.5 µm of a suitable scintillant molecule, the energy of the ß-particle will be sufficient to reach the scintillant and excite it to emit light. If the distance is greater than 1.
5 µm, the ß-particle will not have sufficient energy to travel the required distance to reach the scintillant. In an aqueous solution, collisions of the ß-particles with water molecules dissipate the energy and prevent stimulation of the scintillant.
SPA in action
In SPA, the scintillant is incorporated into small fluomicrospheres or beads. If a radioactive molecule is bound to the bead it is brought in clo enough proximity that it can stimulate the scintillant to emit light.
The beads are designed and constructed to bind specific molecules, through a wide range of bead coatings.
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A wide range of applications is supported, including receptor binding, enzyme, molecular interaction and radioimmunoassays.
SPA bead types
∙ SPA beads can be subdivided into two groups, bad on the wavelength of light emitted
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and subquent detector ud:
∙ SPA imaging beads SPA scintillation beads
Summary of features and benefits
Working with a technology leader affords you veral benefits.
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∙ Standard and custom workstation configurations – choo a single or multiple workstations
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∙ Access to cutting-edge development of new process for industry and academic rearch
∙ Worldwide rvice and support
