pcDNA3.1(+)
pcDNA3.1(-)
Catalog nos. V790-20 and V795-20, respectively
Version I
081401
28-0104
tech_
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Table of Contents
Table of Contents (iii)
Important Information (v)
Purchar Notification (vi)
Methods (1)
Overview (1)
Cloning into pcDNA3.1 (2)
Transfection (6)
Creation of Stable Cell Lines (7)
Appendix (10)
pcDNA3.1 Vectors (10)
pcDNA3.1/CAT (12)
Technical Service (13)
References (15)
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Important Information
Contents pcDNA3.1 is supplied as follows:
Catalog no.Contents
V790-2020 µg pcDNA3.1(+), lyophilized in TE, pH 8.0
20 µg pcDNA3.1/CAT, lyophilized in TE, pH 8.0
V795-2020 µg pcDNA3.1(-), lyophilized in TE, pH 8.0
20 µg pcDNA3.1/CAT, lyophilized in TE, pH 8.0 Shipping/Storage Lyophilized plasmids are shipped at room temperature and should be stored at -20°C.
Product Qualification Each of the pcDNA3.1 vectors is qualified by restriction enzyme digestion with specific restriction enzymes as listed below. Restriction digests must demonstrate the correct banding pattern when electrophored on an agaro gel. The table below lists the restriction enzymes and the expected fragments.
Vector Restriction Enzyme Expected Fragments (bp) pcDNA3.1(+)Nhe I
Pst I
Sac I
5428
1356, 4072
109, 5319
pcDNA3.1(-)Nhe I
Pst I
Sac I
5427
1363, 4064
169, 5258
pcDNA3.1/CAT Nhe I
Pst I
Sac I
6217
2145, 4072
109, 6008
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