doi:10.3969/j.issn.1000⁃484X.2019.02.011
川芎嗪抑制NF⁃κB P65磷酸化对LPS 诱导的骨关节炎软骨细胞凋亡和炎症反应的调节作用①
画碟红萝卜面朱海泉 刘子敏 孟祥圣 孙 晓 王黎明②
(南京医科大学连云港临床医学院急诊外科，连云港222002)
中图分类号 R684.3 文献标志码 A 文章编号 1000⁃484X (2019)02⁃0181⁃05
①本文受江苏省青年基金资助(BK20160423)㊂②南京医科大学附属南京医院骨科，连云港222002㊂作者简介：朱海泉，男，硕士，副主任医师，主要从事创伤修复方面的研究㊂[摘 要] 目的：探讨川芎嗪对LPS 诱导的骨关节炎软骨细胞凋亡和炎症反应的影响及机制㊂方法:LPS 诱导骨关节炎模型㊂软骨细胞分为4个组：对照组；川芎嗪(20μmol /L)组;LPS(100ng /ml)组；川芎嗪(20μmol /L)+LPS(100ng /ml)组㊂Hoechst33258染色检测细胞凋亡㊂ELISA 检测一氧化氮(Nitric oxide,NO)㊁肿瘤坏死因子⁃α(TNF⁃α)和白细胞介素(IL)⁃6水平㊂蛋白印迹检测Ⅱ型胶原(collagenⅡ)㊁聚集蛋白聚糖(aggrecan)和基质金属蛋白酶⁃13(MMP⁃13)㊁NF⁃κB P65和p⁃NF⁃κB P65蛋白水平㊂结果：川芎嗪(20μmol /L)可改善LPS 诱导的骨关节炎软骨细胞形态异常及细胞数量的减少㊂LPS 组细胞凋亡率明显高于对照组(P <0.05)㊂川芎嗪可降低LPS 诱导的细胞凋亡率的升高(P <0.05)㊂与对照组相比,LPS 组Ⅱ型胶原和聚集蛋白聚糖表达明显降低,M
MP⁃13表达明显升高(P <0.05)㊂川芎嗪可抑制LPS 诱导的Ⅱ型胶原和聚集蛋白聚糖表达水平的下降及MMP⁃13表达水平的上升(P <0.05)㊂LPS 组NO㊁TNF⁃α和IL⁃6水平明显高于对照组(P <0.05)㊂LPS+川芎嗪组NO㊁TNF⁃α和IL⁃6水平显著低于LPS 组(P <0.05)㊂与对照组相比,LPS 组p⁃P65/P65比值明显升高(P <0.05)㊂川芎嗪可减弱LPS 诱导的p⁃P65/P65比值的上升(P <0.05)㊂结论：川芎嗪通过抑制NF⁃κB P65磷酸化减轻LPS 诱导的骨关节炎软骨细胞凋亡和炎症反应㊂
[关键词] 川芎嗪；骨关节炎；细胞凋亡；炎症;NF⁃κB P65
Ligustrazine regulates LPS⁃induced apoptosis and inflammatory respon of oste⁃oarthritis chondrocytes via inhibiting phosphorylation of NF⁃κB P65
7寸是多大ZHU Hai⁃Quan ,LIU Zi⁃Min ,MENG Xiang⁃Sheng ,SUN Xiao ,WANG Li⁃Ming .Department of Emergency Surgery ,Lianyungang Clinical Medicine Hospital of Nanjing Medical University ,Lianyungang 222002,China
孙的成语>ps扭曲[Abstract ] Objective :To explore the effect of ligustrazine on the LPS⁃induced apoptosis and inflammatory respon of osteoarthritis chondrocytes.Methods :Osteoarthritis model was induced by LPS.Chondrocytes were divided into four group:control group,ligustrazine(20μmol /L)group,LPS(100ng /ml)group and ligustrazine(20μmol /L)+LPS(100ng /ml)group.Apoptosis was me
asured by Hoechst33258staining.The levels of nitric oxide(NO),tumor necrosis factor α(TNF⁃α)and interleukin (IL)⁃6were detected by ELISA.The protein levels of collagenⅡ,aggrecan,matrix metalloproteina 13(MMP⁃13),NF⁃κB P65and p⁃NF⁃κB P65were tested by Western blot.Results :The LPS⁃induced abnormal cell morphology and decread number of cells were ameliorated by ligustrazine(20μmol /L).The apoptosis in LPS group was higher than control group(P <0.05).The LPS⁃induced enhancive apoptosis was reduced by ligustrazine(P <0.05).Compared with control group,the expression of collagenⅡand aggrecan was alleviated with incread expression of MMP⁃13(P <0.05).The LPS⁃induced declined expression of collagenⅡand aggrecan and elevated expression of MMP⁃13was inhibited by ligustrazine(P <0.05).The levels of NO,TNF⁃αand IL⁃6in LPS group were higher than control group(P <0.05).The levels of NO,TNF⁃αand IL⁃6in LPS+ligustrazine group were lower than LPS group(P <0.05).Compared with control group,the rate of p⁃P65/P65in LPPS group was enhanced(P <0.05).The LPS⁃indiced incread rate of p⁃P65/P65was decread by ligustrazine(P <0.05).Conclusion :Ligustrazine alleviates the LPS⁃induced apoptosis and inflammatory respon of osteoarthritis chon⁃drocytes via inhibiting phosphorylation of NF⁃κB P65.[Key words ] Ligustrazine;Osteoarthritis;Cell apoptosis;Inflammation;NF⁃κB P65
我是个医生 骨关节炎是最常见的慢性关节疾病，涉及炎症
网络编程技术安徽旅游景点和关节结构改变，会出现疼痛甚至关节功能障碍[1]㊂全世界60岁以上的人中约有10%的男性和18%的女性患有骨关节炎㊂骨关节炎是造成世界范围内疼痛㊁残疾和社会经济负担的主要原因[2]㊂骨关节炎治疗是医疗健康事业的重大课题，开发有效
的骨关节炎治疗方法迫在眉睫㊂草药被运用于骨关㊃181㊃朱海泉等 川芎嗪抑制NF⁃κB P65磷酸化对LPS 诱导的骨关节炎软骨细胞凋亡和炎症反应的调节作用 第2期
