Reagents needed:
121.1 g | Tris ba三视图 |
700 ml | ddH2O |
数字化教学>整式的加减70 mltangyuan | Concentrated HCl (37.2% - 12.1M) |
| |
** NOTE: Many electronic pH Meters are unable to accurately determine the pH of concentrated Tris solutions. Be sure to u an appropriate probe. **
Directions:
陈工孟1) Mix 121.1 g of Tris ba with 700 ml of ddH最后一次讲演2O by stirring.
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2) Add HCl in the following volumes to reach the desired pH:
pH 7.4 = 70 ml of HCl; pH 7.6 = 60 ml of HCl; pH 8.0 = 42 ml of HCl
3) Fine adjust to the desired pH (7.4 - 8.0) with concentrated HCl.
4) Add ddH中国复兴之路2O until final volume is 1 L.
5) Autoclave to sterilize.
