盐水鸭肝三、重要科学发现和主要技术发明、科技创新
1.重要科学发现、主要技术发明、主要科技创新重要科学发现
（1）房水抽吸联合激光房角光凝法建立青光眼大鼠模型，重点观察其眼压改变
特征和病理改变特点，探讨其科研应用的可行性。研究结果如下表（图）：
活动议程Figure 1.The photograph of the anterior chamber after photocoagulation of angle under the slit-lamp microscope:The edema of cornea and congestion of conjunctiva were displayed.Anterior chamber form
ed in the first day after operation;Cornea and lens were clear,and vascular network on iris was visible on 3d postoperation.
Table 1
IOP of rat eyes after photocoagulation （x ±s ,mmHg,n =75）
Eyes Pre-op IOP in post-op
1d 3d 7d 14d 21d 28d 35d 42d Right 16.56±1.8421.33±2.64
cb 25.56±2.35cb
对领导班子的意见和建议30.31±3.22
cb
25.67±2.32
cb
21.19±2.18
cb
17.08±1.36c
16.14±1.5216.28±1.58Left
16.70±1.93
17.55±1.58b
16.83±1.34
17.45±1.3716.97±1.1517.67±0.94
16.36±1.60
16.23±1.55
16.34±1.57
F group =122.952,P =0.000;F time =278.146,P =0.000；c P <0.01vs respective left eyes,b P <0.05vs respective pre-op value
Fig.2(A)Line graph:Reprentative IOP in each group within each week.**P<0.01indicated
significantly differed from the IOP baline by ANOVA.(B)Bar graph:Reprentative Variation in intraocular pressure (IOP)at different time points before and after treatments.*P<0.05and**P<0.01ind
icated significantly difference before and after treatment by ANOVA.
Fig.3H&E stain of rat eye at different time （×400）A-E:The angle of the anterior chamber gradually narrow,and trabecular meshwork space compresd even disappear.The edema and curl were in 1,2,4,6,8weeks after operation in the right eyes F-H:No obvious changes in the chamber angle,trabecular meshwork space and iris in 4,6and 8weeks after operation in the left Fig.3Nissl staining of rat retinal （×1000）A:A lots of RGCs were en in the right eye before photocoagulation B-E:The numbers of RGCs in the right eye were gradually decread with increa of time after operation F-J:No obvious change was en in the numbers of retinal ganglion cells left eye before and after operation
A
B
Fig.4(A)Nissl Staining of RGCs in rats (×1000)lots A C D E
B F G H
A B C D F
E G
H
I
J
photocoagulation.The numbers of RGCs in the right eyes were gradually decread with time extension after operation.No obvious change was en in the numbers of retinal ganglion cells in the left eye before and after operation.(B)Bar graph:Reprentative variation in numbers of retinal ganglion cells at different time points.The data were expresd as the mean±SD of independent experiment.Statistical analysis was performed using a one-way ANOVA(*indicates p<0.05and**indicates p<0.01).
A
B
Fig.5Retrograde labeling fluorescent gold of Rat RGCs（×1000）RGC labeled by FG：RGCs were l
abeled with FG in cytoplasm and the boundary was clear.Some cells could be en in the cell apoptosis process,and there was no fluorescent dye leakage.(B)Bar graph:The number of retinal ganglion cells （RGCs）decread with time,*P<0.05and**P<0.01indicated significantly difference before and after treatment
上述研究结果发现实验眼激光术后6次眼压均值较术前明显升高，差异有统计学意义(P< 0.01)；对照眼眼压较术前无明显变化(表1)，实验组可见少数RGCs内灰蓝色Nissl小体减少，甚至消失，而整个细胞染色均匀。光镜下见实验眼较对照眼RGCs数目明显减少
(图4)，逆行标记荧光金染色显示结果基本相似（图5）。RGCs死亡从术后7d开始出现，直至术后56d，死亡高峰在术后28~56d(表2)。
A
B
一同造句
C
Fig.6(A)Microglia cells were stained with CD11b in rat retinal (×1000).7days after chronic ocular hyp
ertension was made in SD rats,the expression of SD was obrved in the experimental group.(B)Bar graph:The expression of CD11b in retinal ganglion cells was significantly changed,and the expression of retinal ganglion cells was significantly incread in 14days,28days,56days,and the number of cells in the retina was not significantly incread.（C ）Green fluorescence:microglia Red fluorescence:TNF-a Yellow:CD11b (microglia)and TNF-a co location
上述研究结果发现：青光眼早期伴随着视网膜节细胞死亡并贯穿整个过程。小胶质细胞通过分泌TNF-a和IL-1B等因子诱导炎症反应，加重神经元损伤，促进节细胞的凋亡。特点表现为细胞形态改变，分布改变和比率增加。应用雷公藤甲素等作用于动物模型，均有保护神经节细胞的作用。
其主要原因是抑制小胶质细胞的活性。
怀念家乡的诗句
（2）雷公藤甲素在慢性青光眼大鼠模型中对视网膜神经节细胞的保护作用机制研究表明，
雷公藤甲素能够抑制中枢神经系统小胶质细胞活化。因此，本研究将雷公藤甲素用于慢性青光眼大鼠模型，观察RGCs存活情况的改变，探讨雷公藤甲素治疗青光眼的潜在可能性。
表3激光光凝大鼠房角青光眼造模后眼压变化（⎺x±s ，mmHg ）
战争与和平作文
时间
样本量雷公藤甲素组生理盐水组（眼数）
激光眼
对照眼
激光眼
对照眼
术前3216.42±1.3317.41±1.4816.56±1.8416.7±1.93术后1d 3221.29±2.2617.72±1.3521.33±2.6417.5±1.58术后2d 3224.94±2.1818.94±1.3025.56±2.3516.8±1.34术后1周3229.06±2.3921.50±1.4630.31±3.2217.4±1.37术后2周3225.13±2.0118.91±0.9325.67±2.3216.9±1.15术后3周3221.03±1.9217.72±0.7521.19±2.1817.6±0.94术后4周3217.22±0.8616.63±1.3417.08±1.3616.3±1.60术后5周3216.25±1.4916.50±1.3016.14±1.5216.2±1.55术后6周3216.44±1.2916.72±1.2216.28±1.5816.3±1.57术后7周3216.19±1.7316.56±0.9216.02±1.8316.1±1.62术前8周3216.42±1.3317.41±1.4816.56±1.8416.7±1.9
3注：F 时间=124.48，P ＜0.01；F 激光=224.56，P ＜0.01；F 组别=0.32,P =0.57；F 时间·激光=33.35,P ＜0.01；F 激光·组别=4.27，P =0.04；F 时间·组别=1.34，P=0.22(三因素方差分析，LSD-t 检验)
恶魔头像
Figure.7Retinal Nissl staining at different time points in model rats (×1000)in normal saline group the number of RGCs decread gradually with the passage of time,8weeks of sporadic,number of the corresponding time points of triptolide group RGCs significantly reduced.A:lar model of
preoperative B:physiological saline group:1weeks after operation;2week C:saline group after 4weeks;D:saline group after 8weeks;E:normal saline group after F triptolide 1weeks after operation G:triptolide group after 2weeks H:triptolide group after 4weeks,J:8weeks after operation by Triptolide
表4激光光凝大鼠房角青光眼造模后RGCs 计数（⎺x±s ，cells/0.024mm²）
红黄蓝宝宝之歌术后时间样本量雷公藤甲素组生理盐水组（周）（鼠）激光眼对照眼激光眼对照眼
1
2468.17±9.2867.00±6.4259.67±5.6867.33±4.762
48
66.67±11.79
68.33±12.25
58.83±7.67
67.25±6.30
