中文摘要
目的:
五年级上册练习题
在传统中医理论指导下，研究中药安神方对酒精成瘾小鼠条件性位置偏爱（CPP)、行为敏化及小鼠中脑边缘区域神经递质多巴胺(DA)、谷氨酸(Glu)和γ-氨基丁酸（GABA）浓度的影响，并初步探讨其对酒精成瘾的干预机制。以期为中草药复方防治酒精成瘾提供理论依据和新的治疗思路。
方法:
SPF级昆明种小鼠，雄性，223g，随机分为盐水组（S+S）、酒精组（S+E）、中药组(Z+S)和中药干预组（Z+E）四组。实验前12h禁食不禁水。中药组(Z+S)、中药干预组（Z+E）分别给予安神方，盐水组（S+S）、酒精组（S+E）给予相同体积的生理盐水，30min后，除盐水组（S+S）及中药组(Z+S)给予生理盐水外，其余各组均给予酒精灌胃建立CPP和行为敏化小鼠模型。观察CPP实验中小鼠在中药干预前后，小鼠在伴药箱（白箱）停留时间，评价安神方对酒精诱导CPP 形成的影响。计数行为敏化实验中小鼠的自主活动情况，评价安神方对小鼠酒精行为敏化获得和表达以及其自身对小鼠自主活动的影响。SPF级昆明种小鼠，雄性，223g，随机分为盐水组（S+S）、酒精组（S+E+E）、行为敏化形成中药干预组(Z+E+E)和行为敏化表达中药干预组（S+E+Z+E）四组。给予酒精灌胃建立行为敏化小鼠模型，经盐水、中药或/和酒精激发后断头处死，采用酶联免疫吸附法（ELISA）测定各组小鼠脑边缘区域多巴胺(
DA)、谷氨酸(Glu)和γ-氨基丁酸（GABA）浓度。
结果:
1.中药组(Z+S)小鼠在伴药箱停留时间为239.0±48.50s，和盐水组（S+S）相比无显著差异（P>0.05)。中药干预组（Z+E）小鼠在伴药箱停留时间为239.0±48.50s，明显低酒精组（S+E），两组比较无明显统计学差异(P<0.01)。
2.中药干预组（Z+E）在第1、3、5、7、9 天的自主活动次数为238.9±51.53，232.8±91.34，259.3±98.96，317.2±86.46，324.5±97.25次，明显低于酒精组（S+E），两组比较具有统计学差异(P<0.05或P<0.01)。
3.中药组（Z+S)第1、3、5、7、9 天的自主活动次数分别为21
汽水的英文
4.4±47.68、242.8±5
5.28、247.2±5
6.59、223.1±63.78、216.9±62.07次，和盐水组（S+S）比较差异均无统计学意义(P﹥0.05)。
大杏仁4.第13天中药组（Z+S)经中药激发后的自主活动次数为224.7±4
5.04次，和盐水组（S+S）经中药激发后的自主活动次数差异无统计学意义。
5.第13天中药干预组（Z+E）经酒精激发后的自主活动次数为38
6.4±103.48次，明显低酒精组（S+E）经酒精激发后的自主活动次数，两者比较差异均有统计学意义(P<0.01)。
饮酒古诗6.第13天酒精组（S+E）经中药﹢酒精激发后的自主活动次数为376.0±58.90次，明显低于酒精组（S+E）经酒精激发后的自主活动次数，两组比较具有统计学差异(P<0.01)。
7.酒精组（S+E+E）DA递质浓度为79.5±7.55ng/L，Glu递质浓度为138.5±9.36nmol/L，GABA 递质浓度为11.2±1.52ng/ml，与盐水组（S+S）、行为敏化形成中药干预组(Z+E+E)及行为敏化表达中药干预组（S+E+Z+E）比较差异均具有统计学意义（P<0.05)。
结论:
蒜苗生长过程记录1、安神方自身不诱导小鼠CPP的形成，但可抑制酒精诱导小鼠CPP的形成。
2、安神方单独使用不影响小鼠的自主活动，但可抑制单次急性酒精使用所致的小鼠高活动性，同时可抑制小鼠酒精行为敏化的获得和表达。
3、安神方可降低酒精敏化小鼠中脑边缘区域DA、Glu的含量，增加GABA含量。
4、安神方自身不具有成瘾潜力，治疗酒精依赖效果明确，组方药物安全，可考虑作为中草药治疗酒精成瘾饮料的母液进行推广。
关键词：中药；酒精；行为敏化；条件位置偏爱；多巴胺；谷氨酸(Glu)；γ-氨基丁酸
ABSTRACT
Objective:With the guidance of the theory of traditional Chine medicine ,to obrve the effect of traditional Chine medicine compound Anshen Decoction on conditioned place preference (CPP), behavioral nsitization and the concentration of neurotransmitter dopamine (DA), glutamic acid (Glu) and γ-aminobutyric acid(GABA) in the mesolimbic region of alcohol addiction mice , and to explore its intervention mechanism of alcohol addiction. In order to provide theoretical basis and new treatment ideas for the prevention and treatment of alcohol addiction.
Method: SPF grade Kunming mice, male, 22±3g, were randomly divided into saline group (S+S), alcohol group (S+E), traditional Chine medicine group (Z+S) and traditional Chine medicine intervention group (Z+E) four groups. Before the experiment 12h fasting can not help but water. Trad
itional Chine medicine group (Z+S) and traditional Chine medicine intervention group Z+E were given Anshen Decoction, saline group (s + s), alcohol group (s + e) given equal vo lume of normal saline, 30 min after, in addition to the saline group (s + s) and traditional Chine medicine group Z+S given physiological saline, other groups were given alcohol intragastric administration of CPP and locomotor nsitization in mice model was
沈可乐established. To obrve the effect of CPP test in mice before and after the intervention of traditional Chine medicine, mice in the medicine chest with retention time (white box), evaluation of Anshen Decoction on alcohol induced CPP. Obrvation of the independent activity frequency of behavioral nsitization experiments in mice, evaluation of Anshen Decoction on alcohol induced behavioral nsitization and expression and its own on spontaneous activity of mice. SPF Kunming mice, male, 223g, were randomly divided into saline group (S+S), alcohol group (S+E+E), behavioral nsitization medicine intervention group (Z+E+E) and the expression of behavioral nsitization TCM intervention group (S+E+Z+E) four groups. Given alcohol gavage behavioral nsitization in mice model was established, by salt water, medicine or / and alcohol after excitation were decapitated by enzyme linked immunosorbent assay (ELISA) was ud to determine the concentration of mice mesolimbic area dopamine (DA), glutamic acid (Glu) and gamma aminobutyric acid (GABA).
Results:
1. Traditional Chine medicine group (Z+S) mice in the medicine cabinet with the residence time of 23.9 + 48.50s and saline group (S+S) had no significant difference (P>0.05). Chine medicine intervention group (Z+E) mice in the medicine cabinet with the residence time of 23.9 + 48.50s obviously with low alcohol group (S+ E), the difference between the two groups has statistical significance (P < 0.01).
2. Traditional Chine medicine intervention group (Z+E) in the 1, 3, 5, 7, and 9 days of independent activity frequency for 97.25 238.9 + + 51.53232.8 91.34259.3 + 98.96317.2 + 86.46324.5 +, significantly lower than the alcohol group (s + e), two group differences
were statistically significant (P < 0.05 or P < 0.01).
3. The Chine medicine group (Z+S) 1, 3, 5, 7, 9 days of spontaneous activity times were 21.44 + 47.68, 242.8 + 55.28, 2
冰臼群
4.72 + 56.59, 223.1 + 63.78, 216.9 + 62.07 times and saline group (S + S), the difference had no statistical significance (P > 0.05).
欢乐颂王子文
4.The 13th day ,the traditional Chine medicine group (Z+S), the number of spontaneous activity after the traditional Chine medicine challenge was 224.7 + 4
5.04 times, and the saline group (S+S) after the spontaneous activity of the number of independent activities after the traditional Chine medicine was not statistically significant.
5. On the 13th day of the traditional Chine medicine intervention group Z+E by alcohol after excitation frequency of autonomic activities for 103.48 38
6.4 + and obviously low alcohol group (S+ E) by alcohol after excitation independent activity frequency, both comparative difference had statistical significance (P < 0.01).
6. The 13th day, alcohol group (S+ E) by Chine herbal medicine + alcohol after excitation independent activity frequency is 376.0±58.90 times, significantly lower than the alcohol group (S+E) by alcohol after excitation independent activity frequency. The difference between the two groups were statistically significant (P < 0.01).
7. The DA neurotransmitter concentration of alcohol group (S+E+E) was 79.5 + 7.55ng/L, Glu neurotransmitter concentrations was 13.85 + 9.36nmol/L and GABA concentration was 11.2 + 1.52n
g/ml, and saline group (S + S), behavioral nsitization nsitization behavior and Chine medicine intervention group (Z+E+E) expression of traditional Chine medicine intervention group (S+E+Z+E) differences were with
