Biochemistry Test （part one）
Name Student ID Score
I Write the Structures and state their applications respectively in Biochemistry. (5 x 4 = 20)
Number | Structure | Applications |
1 | dicyclohexylcarbodiimide | 多肽合成中氨基酸单体的活化试剂或缩水试剂。 |
2 | cyanogen bromide | 特异性断裂甲硫氨酸C-内存涨价端肽键 |
3 | aspartyl protea active site 两个天冬氨酸 | 构成天冬氨酸蛋白酶的活性位点。 |
4 | ATP | 能量分子，磷酸基团供体 |
5 | cAMP | 蛋白激酶A的活化信使 |
| | |
II Matching Questions (30 x 1 = 30)
U the items listed in the table to answer questions 1-15. Not all of the answers will be ud.
| A | B | C | D | E |
1 | zymogen activation | phosphorylation | kinas | liga | phosphatas |
2 | evolutionary tree | convergent | divergernt | thalasmia | sickle-cell |
3 | centrifugation | ion exchange | affinity | gel filtration | precipitation |
4 | synonyms | anonymes | cleft | inner | surface |
5 | competitive | noncompetitive | uncompetitive | feedback | feedforward |
6 | turnover number | covalent bond | salt bridge | van der Waals | asparagine |
阿基米德原理7 | cysteine | histidine | arginine | aspartate | valine |
8 | γ-carboxyglutamate | tyrosine | lysine | rine | hydroxyproline |
9 | ATP | cAMP | 吊车支腿口诀 cGMP | homotropic | heterotropic |
10 | primary | condary | tertiary | negative国语运动 | positive |
11 | restriction endonucleas | orthologs | paralogs | adult | fetal |
12 | superoxide | Bohr effect | SD quence | gla domain | fingerprint |
13 | Ramachandran | Sanger | Chargaff | myoglobin | metmyoglobin |
14 | isozymes or isoenzymes | Ribozyme | allosteric | 交通古今异义cooperative | noncooperative |
15 | stopped-flow | RNAi | gene disruption | Zn2+ | Mg2+ |
16 | rever trancripta | DNA polymera | RNA polymera | Fe+2 | Fe+3 |
17 | gene expression | gene transcription | mRNAs | tRNAs | rRNAs |
18 | site-directed mutagenesis | elagation | relea | expression | cloning |
| | | | | |
1. Some inverted repeat quences or palindromic quences can be specifically cleaved by ____11A_________.
2. The process by which very different evolutionary pathways lead to a protein with similar function is referred to as __2B___ evolution.
3. Under normal conditions, the iron of heme in myoglobin and hemoglobin is in the ___16D____ oxidation state.
4. Carbon dioxide reacts with the amino terminal groups of hemoglobin to form carbamate groups, which carry a ___10D___ charge.
5. In normal adult hemoglobin, HbA, the β6 position is a glutamate residue, whereas in sickle-cell hemoglobin, HbS, it is a __7E___ residue.
6. The T-state of hemoglobin is stabilized by a salt bridge between β1 Asp 94 and the C-terminal ____7B____ of the β1 chain.
7. In trypsin, the specificity pocket contains a/an ____7D____ residue that binds to amino acid residue carrying positive charge of the substrate.
8. In proteas such as papain, a _____7A___ residue is activated by hydrogen-bonding to a histidine residue.
9. Multiple forms of homologous enzymes within the same organism that catalyze the same reaction but with different kinetic properties are ____14A____.
10. The effects of substrates on allosteric enzymes are referred to as _____9D_____ effects.
11. ___9A___ is the most common donor molecule in phosphorylation reactions catalyzed by protein kinas.
12. α helices, β sheets, and turns are referred as__10B___ structure of proteins.
13. The purification bad on the attraction of the protein for a particular chemical group is ___3C__ chromatography.
14. The information-carrying intermediates for translation are _17C_.
15. The person who noted the A:T and G:C ratios in DNA are approximately 1:1 is _13C_.
16. Codons that specify the same amino acids are termed __4A__.
17. A pattern of DNA fragments by restriction digestions can rve as a _12E of a particular DNA molecule.
18. Glutamine substitution with __6E__ is an example of a conrvative amino acid change.
19. The decread production of one of the subunits of hemoglobin can cau _2D__.
20. Two α chains and two γ chains are ud to constitute ___11E___ hemoglobin.
21. The kcat is often referred to as the _6A__.
22. Enzymes that do not obey Michaelis-Menten kinetics are _14C__.
23. The activity of carbonic anhydra requires metal ion __15D__.
24. We can u __18A___ to determine structure/function relationships in enzymes.
25. We usually refer the inhibition of the first step by the final product of the pathway as__5D__inhibition.
26. Activation of prothrombin requires modified amino acid __8A__ in gla domain.
27. The enzyme ud to create DNA from RNA is 16A .
28. Removal of phosphates from proteins is catalyzed by protein __1E__.
29. Ω, β turns and loops are often found on 4E of proteins.
30. Blood clotting cascades are controlled by __1A_.
III Fill in the Blanks (2 x 10 = 20)
1. The aromatic amino acids in proteins are Phe, Tyr, and Trp .
2. During protein purification, both 测定比活性和回收率 are necessary each step.
3. Monoclonal antibodies are made by 生产抗体的B细胞与小鼠骨髓瘤细胞融合 形容人聪明的词语.
4. Semi-conrvative replication of DNA was confirmed by M. Melson and F. W. Stahl experiment(s).
5. Distal histidine in hemoglobin donates 商务车有哪些hydrogen bond to oxyhemoglobin complex, which inhibits the oxidation of the iron to the ferric state.
6. α-hemoglobin is stabilized by AHSP .
7. The meaning of Vmax catalyzed by an enzyme is 所有活性位点都与底物结合时的反应初速度 .
8. An affinity label of an enzyme is 有反应活性基团的底物类似物 .
