Ovarian tissue cryoprervation for patients with premature ovary insufficiency caud by cancer treatment: optimal protocol
期刊名称: Climacteric有关泰山的诗句>地瓜干热量
作者: Li, Y.,Ruan, X.,Liebenthron, J.,Montag, M.,Zhou, Q.,Kong, W.,Du, J.,Jin, F.,Li, S.,Cheng, J.,Wang, H.,Mueck, A. O.
年份: 2019年
关键词: Ovarian tissue cryoprervation;N-acetyl-l-cysteine;follicle viability;DNA fragmentation;reactive oxygen species;total antioxidant capability
喉音12色吧摘要:Objective: Premature ovary insufficiency is frequent after chemotherapy/radiotherapy in cancer patients. Ovarian tissue (OT) cryoprervation and later retransplantation, the routine method in Europe, has recently been implemented at the first center in China. We investigated the protective effect of the antioxidant N -acetyl- l -cysteine (NAC) during the decisive freezing–thawing steps. Metho
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机遇名言ds: Fifteen OT samples were obtained from each of 13 cancer patients prospectively and randomly assigned to a control group and four groups with different NAC concentrations (Group 1, 0 mM NAC; Group 2, 0.5 mM NAC; Group 3, 1 mM NAC; Group 4, 5 mM NAC; Group 5, 25 mM NAC). After thawing, the follicle viability, DNA fragmentation, levels of reactive oxygen species (ROS), and total antioxidant capacity (TAC) were evaluated. Results: OT...
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