戏剧种类叶酸受体介导的姜黄素纳米混合胶束的制备、理化性质考察及体外抗肿瘤的初步研究
摘要：
目的：制备一种具有较高靶向性和抗肿瘤效应的叶酸受体介导的姜黄素纳米混合胶束，并考察其理化性质和体外抗肿瘤作用。
方法：叶酸-DSPE-PEG2000和姜黄素-DSPE-PEG2000复合物通过薄膜分散法制备纳米混合胶束。采用动态光散射和透射电子显微镜测定胶束粒径和形态，测定胶束吸光度和制备纯度，表征药物载量和药物释放性能。通过MTT法和细胞凋亡率检测法评价胶束对MCF-7细胞的抑制作用和促进细胞凋亡的能力。
结果：制备的纳米混合胶束粒径为（132.7 ± 5.3）nm，载药量为（17.3 ± 1.2）％，药物释放速率为30 h内释放了57.8％的姜黄素。体外实验结果显示，与未经修饰的姜黄素纳米粒子相比，修饰了叶酸的纳米混合胶束能够更有效地抑制MCF-7细胞的增殖，选择性地靶向肿瘤细胞，并通过促进细胞凋亡的机制发挥抗肿瘤作用。
个人能力结论：叶酸受体介导的姜黄素纳米混合胶束的制备成功，能够提高药物在肿瘤细胞中的选址
性和治疗效果。
关键词：纳米混合胶束，叶酸受体，姜黄素，体外抗肿瘤，选址性
Preparation, Physicochemical Characterization, and Preliminary In Vitro Antitumor Study of Folate Receptor Targeted Curcumin Nanohybrid Micelles黄山的四绝是什么
Abstract:
Objective: To prepare a novel folate receptor targeted curcumin nanohybrid micelle with high targeting ability and antitumor effect, and investigate its physicochemical properties and in vitro antitumor activity.
Methods: Folate-DSPE-PEG2000 and curcumin-DSPE-PEG2000 composites were fabricated into nanohybrid micelles by thin film hydration method. The size and shape of micelles were determined by dynamic light scattering and transmission electron microscopy. The micelle purity and drug loading capacity were characterized by UV-Vis spectrophotometer. The drug relea behavior was also studied. The inhibitory effect and
apoptosis-inducing ability of micelles on MCF-7 cells were evaluated by MTT assay and apoptosis rate analysis.
Results: The nanohybrid micelles had an average size of (132.7 ± 5.3) nm, drug loading capacity of (17.3 ± 1.2) %, and a drug relea rate of 57.8% within 30 hours. Compared with non-modified curcumin nanoparticles, folate receptor targeted nanohybrid micelles displayed enhanced inhibition on MCF-7 cells, improved tumor lectivity, and exerted antitumor effect by inducing apoptosis.
Conclusion: Folate receptor targeted curcumin nanohybrid micelles were successfully prepared and showed superior targeting ability and antitumor efficacy.
qq状态Keywords: nanohybrid micelles, folate receptor, curcumin, in vitro antitumor, tumor lectivit
Curcumin, a natural polyphenol found in turmeric, has shown promising anticancer activity in various preclinical studies. However, its limited bioavailability and lack of tumor
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lectivity have hindered its clinical translation. To overcome the limitations, we developed folate receptor targeted nanohybrid micelles encapsulating curcumin for enhanced tumor lectivity and anticancer efficacy.
In this study, we first synthesized curcumin nanoparticles using a precipitation method and then modified them with a folate-conjugated lipid to target folate receptor overexpressing cancer cells. The resultant nanohybrid micelles exhibited a nanosize range of 75-105 nm and high drug-loading efficiency of 18.4%. We further evaluated the in vitro anticancer activity of the nanohybrid micelles on MCF-7 breast cancer cells.ps抠印章
The results showed that folate receptor targeted nanohybrid micelles significantly inhibited the proliferation of MCF-7 cells in a do-dependent manner, with an IC50 value of 11.5 μg/mL within 30 hours of incubation. Moreover, compared to non-modified curcumin nanoparticles, folate receptor targeted nanohybrid micelles displayed enhanced inhibition on MCF-7 cells, indicating the superior targeting ability of the nanohybrid micelles.
In addition, we investigated the mechanisms of action of folate receptor targeted nanohybrid micelles on MCF-7 cells. The nanohybrid micelles induced apoptosis in MCF-7 cells, as evidenced by the incread percentage of apoptotic cells, upregulation of pro-apoptotic Bax protein, and downregulation of anti-apoptotic Bcl-2 protein.
In conclusion, folate receptor targeted curcumin nanohybrid micelles were successfully prepared and showed superior targeting ability and anticancer efficacy. The development of such targeted nanohybrid micelles may facilitate the clinical translation of curcumin for improved cancer treatment
In addition to its anticancer properties, curcumin has been shown to have a variety of other health benefits. For example, it has anti-inflammatory effects and can help to alleviate symptoms of inflammatory dias such as arthritis and irritable bowel syndrome. Curcumin has also been shown to have neuroprotective effects and may be uful in the treatment of Alzheimer's dia and other neurodegenerative disorders.
Despite its many potential benefits, curcumin has limited bioavailability, meaning that it is
poorly absorbed by the body when taken orally. This has been a major barrier to the development of curcumin as a therapeutic agent. However, the u of targeted nanohybrid micelles has shown promi in improving the bioavailability and efficacy of curcumin.
百灵火In addition to folate receptor targeted nanohybrid micelles, other types of targeted delivery systems for curcumin are also being investigated. One example is the u of nanoparticles made from biocompatible and biodegradable polymers such as poly(lactic-co-glycolic acid) (PLGA) or chitosan. The nanoparticles can be coated with molecules that bind specifically to cancer cells, allowing for targeted delivery of curcumin to the cells.
