pDsRed-Monomer-Golgi
pDsRed-Monomer-Golgi 荧光蛋白报告载体基本信息：
质粒类型: 荧光蛋白报告载体
启动子:
CMV 载体大小:
4.9kb 载体抗性: Kanamycin (卡那霉素)
感恩父母亲筛选标记: Neomycin (新霉素)
pDsRed-Monomer-Golgi 载体质粒图谱和多克隆位点信息：
电子针灸
pDsRed-Monomer-Golgi 载体简介：
pDsRed-Monomer-Golgi encodes a fusion protein consisting of DsRed-Monomer, a monomeric mutant derived from the tetrameric Discosoma sp. red fluorescent protein DsRed (1), and a quence encoding the N-terminal 81 amino acids of human beta 1,4-galactosyltransfera (GT; 2).
This region of human beta 1,4-GT contains the membrane-anchoring signal peptide that targets the fusion protein to
the
新疆的美食trans-medial region of the Golgi apparatus (3–5).
pDsRed-Monomer-Golgi encodes a fusion protein consisting of DsRed-Monomer, a monomeric mutant derived from the tetrameric Discosoma sp. red fluorescent protein DsRed (1), and a quence encoding the N-terminal 81 amino acids of human beta 1,4-galactosyltransfera (GT; 2). This region of human beta 1,4-GT contains the membrane-anchoring signal peptide that targets the fusion protein to the trans-medial region of the Golgi apparatus (3–5).
SV40 polyadenylation signals downstream of the DsRed-Monomer-Golgi fusion direct proper processing of the 3' end of the mRNA. The vector backbone also contains an SV40 origin for replication in mammalian cells expressing the SV40 T-antigen. A neomycin resistance castte (Neor ) consisting of the SV40 early promoter, the neomycin/kanamycin resistance gene of Tn5, and polyadenylation signals from the herpes simplex virus thymidine kina (HSV-TK) gene allow stably transfected eukaryotic cells to be lected using G418 (7). A bacterial promoter upstream of this cas
tte drives expression of the gene encoding kanamycin resistance in E. coli. The pDsRed-Monomer-Golgi backbone also provides a pUC origin of replication for propagation in E. coli and an f1 origin for single-stranded DNA production.
pDsRed-Monomer-Golgi载体序列
pDsRed-Monomer-Golgi其他相关荧光蛋白报告载体：打的多音字组词
pEBFP-Mito
pEGFP-Mem
pIRES2-DsRed-Express
pcDNA3.1-EGFP
pDsRed-Monomer-Golgi
pDsRed1-N1
pECFP-MEM
尿常规能检查出什么
pECFP-Nuc
pIRES-hrGFP-1a
pRL-CMV
pDsRed2-1
关键时刻掉链子pEYFP-Mito
pCMS-EGFP
卡通乌龟图片pECFP-Peroxi
pEGFP-C2
pEGFP-N2
pEGFP-Tub
pEYFP-C1
pEYFP-ER
pEYFP-N1
pPAmCherry-Tubulin
pZsYellow-N1 pAmCyan-N1 pDsRed-Monomer-N1 pDsRed1-C1 pDsRed2-Mito pDsRed2-N1 pDsRed2-Nuc pEBFP-N1
pECFP-C1
pEGFP-1
pEGFP-Actin pEGFP-C1
pEGFP-C3
pEGFP-N1
pEGFP-N3
pEYFP凉拌黄瓜片
pIRES2-DsRed2 pIRES2-EGFP pLEGFP-C1 pmCherry-C1 pmCherry-N1
