Differential integrin activity mediated by platelet collagen receptor engagement under flow
conditions
期刊名称： Thromb Haemost
作者： Pugh Nicholas,Maddox Ben,Bihan Dominique,Taylor Kirk,Mahaut-Smith Martyn,Farndale Richard
年份： 2017年
期号： 第08期
关键词： Platelet glycoproteins;collagen receptors;signal
transduction;thrombosis;fluid shear
摘要：The platelet receptors glycoprotein (Gp)VI, integrin α2β1and GpIb/V/IX mediate platelet adhesion and activation during thrombogenesis. Increas of intracellular Ca2+([Ca2+]i) are key signal
s during platelet activation; however, their relative importance in coupling different collagen receptors to functional respons under shear conditions remains unclear. To study shear-dependent, receptor-specific platelet respons, we ud collagen or combinations of receptor-specific collagen-mimetic peptides as substrates for platelet adhesion and activation in whole human blood under arterial flow conditions and compared real-time and endpoint parameters of thrombus formation alongside
[Ca2+]imeasurements using confocal imaging. All three collagen receptors coupled to [Ca2+]isignals, but the varied in amplitude and temporal
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