pBlueScript SK(+)载体说明

pBlueScript SK(+)

编号 载体名称

北京华越洋生物VECT4780

pBlueScript SK(+)

pBlueScript SK(+)载体基本信息

载体名称:

pBlueScript范例教学理论 SK(+)

质粒类型: 噬粒载体

高拷贝/低拷贝: 高拷贝

启动子:

Lac

克隆方法: 多克隆位点，限制性内切酶

载体大小:

2958bp

5' 测序引物及序列:

M13-‐F: TGTAAAACGACGGCCAGT

3' 测序引物及序列:

M13-‐R: CAGGAAACAGCTATGAC

载体标签: 无

载体抗性: 氨苄

筛选标记:

-‐-‐

备注:

-‐-‐后天气丹

稳定性:

-‐-‐

组成型: 非组成型

病毒/非病毒: 非病毒

pBlueScript SK(+)载体质粒图谱和多克隆位点信息

pBlueScript SK(+)载体序列

ORIGIN

1 CACCTAAATT GTAAGCGTTA ATATTTTGTT AAAATTCGCG TTAAATTTTT GTTAAATCAG

61 CTCATTTTTT AACCAATAGG CCGAAATCGG CAAAATCCCT TATAAATCAA AAGAATAGAC

121 CGAGATAGGG TTGAGTGTTG TTCCAGTTTG GAACAAGAGT CCACTATTAA AGAACGTGGA

181 CTCCAACGTC AAAGGGCGAA AAACCGTCTA TCAGGGCGAT GGCCCACTAC GTGAACCATC

241 ACCCTAATCA AGTTTTTTGG GGTCGAGGTG CCGTAAAGCA CTAAATCGGA ACCCTAAAGG

301 GAGCCCCCGA TTTAGAGCTT GACGGGGAAA GCCGGCGAAC GTGGCGAGAA AGGAAGGGAA

361 GAAAGCGAAA GGAGCGGGCG CTAGGGCGCT GGCAAGTGTA GCGGTCACGC TGCGCGTAAC

421 CACCACACCC GCCGCGCTTA ATGCGCCGCT ACAGGGCGCG TCCCATTCGC CATTCAGGCT

481 GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA

541 AGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG

601 TTGTAAAACG ACGGCCAGTG AATTGTAATA CGACTCACTA TAGGGCGAAT TGGGTACCGG

661 GCCCCCCCTC GAGGTCGACG GTATCGATAA GCTTGATATC GAATTCCTGC AGCCCGGGGG

721 ATCCACTAGT TCTAGAGCGG CCGCCACCGC GGTGGAGCTC CAGCTTTTGT TCCCTTTAGT

781 GAGGGTTAAT TTCGAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT

841 ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG

901 CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG

961 GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC

1021 GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC

1081 GGCGAGCGGT ATCAG元宵节农历 CTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA

1141 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG

1201 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT

1261 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA

1321 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC

1381 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT

1441 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG

1501 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG

1561 CAGCAGCCAC TGGTA唐朝头饰 ACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT

1621 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC

1681 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG

1741 CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC

1801 AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT

1861 AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA

1921 AATGAAGT个人材料 TT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT

1981 GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TC租房合同样式 GTTCATCC ATAGTTGCCT

2041 GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG

2101 CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG

2161 CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA

2221 ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG

2281 CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG

2341 GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT

2401 CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA

2461 TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG

2521 GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC

2581 CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG

2641 GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA

2701 TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG

2761 GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT

2821 GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC

2881 TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA

2941 CATTTCCCCG AAAAGTGC

//

其他大肠杆菌表达载体：

pBV22远离垃圾食品 1 ptdTomato pET-52b(+) pAmCyan

pDsRed-Express2 pBV220 pCold-GST pColdS-SUMO

pCold TF pCold IV pCold III pCold II

pCold I pE-SUMO pCold-ProS2 pBAD102/D-TOPO

pBAD202/D-TOPO pACYC184 pBAD/Thio-TOPO pBad/Myc-His C

pBad/Myc-His B pBad/His B pBad/Myc-His A pBad/His C

pBad/His A pBAD-TOPO pET-23b(+) pET-23a(+)

pET-23c(+) pET-23(+) pET-12b(+) pET-12c(+)

pET-12a(+) pET-11b(+) pET-11a(+) pET-11c(+)

pBad24 pQE-82L pQE-81L pQE-80L

pQE-32 pQE-9 pQE-16 pQE-31

pQE-60 pQE-70 pQE-40 pET-51b(+)

pET-50b(+) pET-49b(+) pET-48b(+) pET-47b(+)

pET-26b(+) pET-32a(+) pET-21b(+) pET-22b(+)

pET-14b pET-16b pET-15b pET-19b

pET-20b(+) pET-21d(+) pET-21c(+) pET-21b(+)

pET-21a(+) pET-24a(+) pET-24d(+) pET-25b(+)

pET-27b(+) pET-28a(+) pET-30a(+) pET-42a(+)

pET-43.1c(+) pET-43.1b(+) pET-43.1a(+) pET-44a(+)

pET-44c(+) pET-46 EK/LIC pET-37b(+) pTrcHis2 C

pTrcHis2 B pTrcHis2 A 肠胃炎吃什么食物 pET303/CT-H励志短句 is pET302/NT-His

pRSET-CFP pRSET-EmGFP pRSET-BFP pGFPuv

pET300/NT-DEST pET301/CT-DEST pGEM-T pBad43

pGEX-4T-3 pGEX-5X-2 pBlueScript SK(+) pG-Tf2

pG-KJE8 pGro7 pET-SUMO pSE380

pET-17b pET102/D-TOPO pCDFDuet-1 pMAL-p5x

pTf16 pET-28c(+) pBluescript II SK(+) pET-30b(+)

pSUMO pProEX HTc pProEX HTb pProEX HTa

pKD3 pKD13 pKD46 pTYB1

pTYB2 pTWIN2 pBluescript II KS(-) pTYB12

pMAL-p5e pACYCDuet-1 pEGM-11ZF(+) pEGM-7ZF(+)

PinPoint Xa-3 PinPoint Xa-2 PinPoint Xa-1 pSP73

pSP64 pTWIN1 pTYB11 pTXB1

pET-5b(+) pBad/gIII C pBad/gIII B pBad/gIII A

pET-5a(+) pMal-p4X pMal-p2G pkk223-3

pkk232-8 pCYB1 pEZZ18 pBAD18

pMAL-c5x pMal-p2E pMal-p2X pET-44 EK/LIC

pET-43.1 EK/LIC pET-41 EK/LIC pMal-c4X pTrcHis B

pET-31b(+) pET-3b(+) pET-41a(+) pGEX-3X

pGEX-4T-2 pETDuet-1 pGEX-4T-1 pTrc99a

pET-28b(+) pET-His pALEX a,b,c pACYC177

pBR322 pKD4 pKD20 pMXB10

pEcoli-6xHN-GFPuv pKJE7 pRSET B pGEX-KG

pGEX-2T pRSFDuet-1 pCOLADuet-1 pTrcHis C

pTrcHis A pET-41b(+) pET-42b(+) pET-3a(+)

pGEX-6P-3 pGEX-6P-2 pGEX-6P-1 pGEX-5X-3

pGEX-5X-1 pGEX-2TK pRSET A pMal-c2G

pMal-c2E pMal-c2X pRSET C pQE-30

pET-45b(+) pET-44b(+) pET-42c(+) pET-41c(+)

pET-40b(+) pET-33b(+) pET-39b(+) pET-32 EK/LIC

pET-32 Xa/LIC pET-32c(+) pET-32b(+) pET-30 Xa/LIC

pET-30 EK/LIC pET-30c(+) pET-29c(+) pET-29b(+)

pET-29a(+) pET-24c(+) pET-24b(+) pET-24(+)

pET-23d(+) pET-11d(+) pBad33