he染色步骤

组织名称wereimmerdin4%paraformaldehydefor4h,andtransferedto70%ethanol.

Individuallobesof组织名称biopsymaterialwereplacedinprocessingcasttes,dehydrated

througharialalcoholgradient,immunostaining,

5-um-thicklungtissuectionswered建军大业背景 ewaxedinxylene,rehydratedthroughdecreasing

concentrationsofethanol,nstainedwithhematoxylinandeosin

(H&E).Afterstaining,ctionsweredehydratedthro提醒的英文 ughincreasingconcentrationsofethanoland

xylene.

Immunohistochemistryfor写作业听的歌 CCR7,CD206,andF4/nswereunmas病假怎么请 kedbymicrowaving

ctionsin10mmol/Lcitratebuffer,pH6.鲜艳近义词 0(15minutes),andimmunostainingwasundertaken

usingtheavidinbiotinylatedenzymecomplexmethodwithantibodiesagainstCCR7ata

concentrationof1ug/ml,CD206ataconcentrationof1ug/ml,F4/80ataconcentrationof1

ug/ml,ncubation

withtheappropriatebiotin-conjugatedcondaryantibodyandsubquentlywithstreptavidin

solution,colordevelopmentwasperformedusing

3,3-diaminobenzidinetetrahydrochloride

(VectorL康熙八子 aboratories)nswerecounterstainedusingGill-2hematoxylin

(Thermo-Shandon,Pittsburgh,PA).Afterstaining,ctionsweredehydratedthroughincreasing

concentrationsofethanolandxylene.