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A new integrative reporter plasmid for
Streptococcus pneumoniae
美术文献期刊名称： Fems Microbiology Letters志士不饮盗泉之水
多肉视频作者： Halfmann Alexander,Hakenbeck Regine,Brückner Reinhold
期号： 第2期谨向
关键词： promoter probe;genomic integration;β-galactosida;promoter fusion
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摘要：A new promoter probe system for Streptococcus pneumoniae has been developed that allows stable genomic integration of promoters cloned in front of a promoterless hybrid -galactosida gene consisting of translation initiation signals of the protea gene htrA of S. pneumoniae fud to a truncated Escherichia coli -galactosida gene lacZ . Chromosomal inrtions of promoter- lacZ fusions are directed to the endogenous -galactosida gene bgaA , thereby abolishing background -galactosida activity. The new system was tested by measuring -galactosida activity directed by the
一位小数two promoters of the early competence genes comA and comC . The new integrative plasmid offers veral advantages compared with existing systems and is especially suited for stable integration of small promoter fragments to conduct
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