The role of TerW protein in the tellurite resistance of uropathogenic Escherichia coli 期刊名称: Biologia
憋尿的危害作者: Lenka Valkovicova,Danka Valkova,Silvia Vavrova,Olga Alekhina,Vinh Phu大学学习方案
Hoang,Margareta Jezna,Jan Turna
急性化脓性腮腺炎>新鲜松茸怎么吃年份: 2011年
期号: 第4期
关键词: tellurite resistance;ter genes;TerW biological function周讯
摘要:The ter operon, which is found on a large conjugative plasmid, pTE53, from the uropathogenic strain Escherichia coli KL53, mediates heavy metal ion resistance to tellurium compounds. Here we prent the function of the terW gene, which is transcribed from its own promoter in the opposite direction to the terZABCDE loci and encodes a protein of 155 amino acids. A TerW protein containing an N-terminal His-tag was overexpresd, purified and analyd for the in vitro DNA-binding. Green flu
学习的重要性作文orescent protein fusions, a fluorimetric assay, nonradioactive gel retardation and capillary electrophoresis footprinting were ud to examine the interaction between the bioinformatically-predicted DNA-binding region and TerW. Here we show that TerW binds specifically to the potential promoter region of the terZABCDE genes, which are responsible
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