Characterization of amyloid beta peptides in cerebrospinal fluid by an automated immunoprecipitation procedure followed by mass
拌粉皮spectrometry.
24节气雨水
期刊名称： Journal of Proteome Rearch
潘安作者： Portelius, Erik,Tran, Ai Jun,Andreasson, Ulf,Persson, Rita,Brinkmalm,
笨女孩安琪儿Gunnar,Zetterberg, Henrik,Blennow, Kaj,Westman-Brinkmalm, Ann
年份： 2007年
期号： 第11期
关键词： Humans;Cerebrospinal Fluid;Alzheimer
腰腹力量Dia;Peptides;Immunotherapy;Cell Culture
Techniques;Immunoprecipitation;Mass Spectrometry;Spectrometry, Mass,
关于牛的资料Matrix-Assisted Lar Desorption-Ionization;Chromatography, Liquid
养殖蜈蚣摘要：Pathogenic events in Alzheimer's dia are believed to involve an imbalance between the production and clearance of the neurotoxic 42 amino acid form of the beta-amyloid peptide (Abeta1-42). Although much is known about the production of Abeta1-42, many questions remain about its degradation. Here, we describe an optimized automated immunoprecipitation mass spectrometry method that enables accurate and rapid monitoring of the major Abeta isoforms in cerebrospinal fluid. Furthermore, we describe a technique of antibody immobilization, minimizing background signals. The identities of the Abeta products were confirmed using matrix-assisted lar desorption/ionization time-of-flight mass spectrometry and nanoflow liquid chromatography and tandem mass spectrometry with a hybrid linear trap Fourier transform ion cyclotron
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