Purification and characterization of a novel estera promising for the production of uful compounds from Microbacterium sp. 7-1W
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志愿活动英语期刊名称： FEMS Microbiology Letters
作者： Honda Kohsuke,Kataoka Michihiko,Ono Hiroko,Sakamoto Keiji,Kita
Shinji,Shimizu Sakayu
期号： 第2期
蓝莓营养成分关键词： Estera;Serine hydrola;Regiolective三国志武帝纪
hydrolysis;Cetraxate;Pantothenate;\n Microbacterium\n太行山南麓
摘要：A novel estera catalyzing regiolective hydrolysis was purified from the membrane fraction of Microbacterium sp. 7-1W, and characterized. The enzyme was solubilized with Brij 58 and purified 13.8-fold to apparent homogeneity with 2.58% overall recovery. The relative molecular mass of the nati
ve enzyme as estimated by gel filtration was more than 600000 Da, and the subunit molecular mass was 62000 Da. The enzyme catalyzed cleavage of the terminal ester bonds of cetraxate esters and pantothenate esters. The K m and V max values for methyl cetraxate were
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0.380 mM and 7.76 μmole min 1 mg 1 protein, respectively. The enzyme was inhibited by rine hydrola inhibitors.
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