山西农业科学2020,48(4):467-476
谷子
基因家族的鉴定及表达分析郭
昱1,李娜1,李珊1,韩渊怀1,2,段明2,马芳芳1,2(1.山西农业大学农学院,山西太谷030801;2.山西农业大学生物工程研究所,山西太谷030801)摘要:谷子是我国重要的粮饲作物,具有抗旱耐贫瘠的特点,OFP 家族是植物特有的一类调节植物生长和发育
居民幸福指数的转录因子,参与调控植物的抗逆性。为了探索谷子OFP 家族基因的特征和功能,通过基因定位、柠檬绿茶
基因结构分析、motif 预测、进化分析与顺式作用元件分析及亚细胞定位预测来解析谷子OFP 家族基因的特征,
香蕉吃了发胖吗分析谷子与拟南芥和水稻基因组中的OFP 家族基因的共线性、选择压力,
最后分析谷子OFP 家族基因的表达模式。结果表明,共筛选到谷子OFP 家族基因34个,分布在8条染色体上;进化分析发现,
谷子OFP 家族基因可以分为3个亚类;motif 预测中共鉴定到8个motif 基序;顺式作用元件分析发现,与干旱响应相关的OFP 基因共22个;与拟南芥的
点心有哪些5个基因、水稻的31个基因存在共线性;18个OFP 家族基因被预测定位到细胞核内;
谷子OFP 家族基因在穗中表达量最多,干旱胁迫下的OFP 基因大多表现下调,具有时空特异性。OFP 家族基因Seita.2G184300、Seita.
7G093000、Seita.3G008900的表达差异可能是造成豫谷1号和安-04抗旱性差异较大的原因之一。结果可为谷子
无线路由桥接OFP 家族基因的功能研究提供基础。
关键词:OFP 基因家族;谷子;抗旱;表达分析中图分类号:S515文献标识码:A 文章编号:1002-2481(2020)04-0467-10
Identification and Expression Analysis of Gene Family in
GUO Yu 1,LI Na 1,LI Shan 1,HAN Yuanhuai 1,2,DUAN Ming 2,MA Fangfang 1,
2(1.College of Agronomy ,
Shanxi Agricultural University ,Taigu 030801,China ;2.Institute of Bioengineering ,Shanxi Agricultural University ,Taigu 030801,China )
Abstract :Foxtail millet is an important foodstuff crop in China.It has the characteristics of drought resistance and barrenness tolerance.The OFP family is a unique transcription factor regulating plant growth and development,and also participates in the regulation of plant stress resistance.This study will preliminarily explore the characteristics and functions of the OFP family genes in foxtail millet.The characteristics of OFP family genes in foxtail millet were analyzed by gene mapping,gene structure analysis,motif prediction,evolutionary analysis,cis-acting element analysis and subcellular localization prediction.At the same time,the collinearity and lective pressure analysis of OFP family genes in foxtail millet,Arabidopsis and rice genomes were analyzed.Finally,we analyzed the gene expression patterns of OFP family in foxtail millet.The results showed that there were thirty-four genes of foxtail millet OFP family were screened and distributed on eight chromosomes.Evolutionary analysis revealed that OFP family genes in foxtail millet could be divided into three subgroups.Eight motifs were identified in motif prediction.Cis-acting element analysis showed that twenty-two genes related to drought respon,the foxtail millet OFP family gene was colinear with five Arabidopsis genes and 31genes of rice,and eighteen OFP family genes were predicted to be located in the nucle
us.The OFP family genes in foxtail millet were most expresd in panicles,and the OFP gene was more down-regulated under drought stress,which had temporal and spatial specificity.The difference of expression of Seita.2G184300,Seita.7G093000and Seita.3G008900may be the cau of the difference of drought resistance between Yugu 1and An-04.This study will provide a basis for functional study of OFP family genes in foxtail millet.
图书馆的英语Key words :OFP gene family;Setaria italica ;drought resistance;expression analysis
收稿日期:2019-10-16基金项目:国家自然科学基金项目(31401396);山西省重点研发计划项目(201803D221019-2,201703D211008);山西省青年科技研究基金项
目(201601D021125);山西农业大学校基金项目(2013YJ04,2014022,2014YZ2-5);山西农业大学科技创新基金项目
生日蛋糕的做法大全>航空联队
(2017GPY01)作者简介:郭昱(1992-),男,河南滑县人,在读硕士,研究方向:植物抗旱生理与分子育种。马芳芳为通信作者。
谷子(Setaria italica )为一年生草本植物,
属于禾本科狗尾草属,大约公元前6000a ,谷子从狗尾
草驯化而来,成为我国北方主要的谷类作物[1]。谷子
具有抗旱、耐贫瘠的特点,在炎热和干燥的月份,仅需要少量的水就可以快速成熟,对不利农业生态条件具有很强的适应性。谷子是生活在干旱、
半干旱467··