提取方法主要有Bligh—Dyer法及Folch法。前者适用于大量脂类的提取，提取率在
绿茶有哪些品种95％甚至更高；后者适用于少量组织内脂的提取，提取率在95％～99％。我们在实验中
常用Folch法。称取1 g待测样品，加入10 ml甲醇，加少量酸洗砂研磨，匀浆1 min，然后
加入20 ml氯仿，继续匀浆2 min，过滤，滤渣中加入氯仿一甲醇混合液(2：1 V／V)30 ml
并研磨，过滤。先用20 ml氯仿及10 ml甲醇洗涤滤渣，合并滤液，然后加入全部滤液体
积1／4的水，振荡，静置分层，将上层及界面处固体物吸除去，然后加入甲醇一水混合物
(1：1)，体积为下层体积的1／4，振荡，静置分层，吸掉上层液体及界面物，重复洗涤两次。
然后将有机相减压蒸干，配制成氯仿溶液，一20℃下保存。注意：在分析植物组织中的脂
苏轼与佛印的故事质时，需要用加热法使植物中的脂酶失活。
1．皂化、酯化
蒜头怎么腌制好吃
取约4 mg的总脂按Carreau—Dubacq法，加入约1 ml 1．5％的NaOH／MeOH(或
1％Na／MeOH)溶液，在55。C水浴中加热30 min，然后加入1．5 ml HCl／MeOH，水浴加
热30 min，加0．5 ml左右的蒸馏水，然后用4 ml／Eg烷萃取，吸取正己烷层，重复两次。
然后将萃取液减压蒸干，加入0．3 ml氯仿。
Modified Folch procedure
Homogenid tissue (10 g) was progressively added to
small amounts of a chloroform/methanol 2:1 (v/v) mixture
(up to 200 ml), with vigorous shaking, and then the
extraction was carried on for a further 2h, using an
electromagnetic stirrer. The mixture was filtered and the
filter was re-washed with fresh solvent and presd.
分化事权
Fifty millilitres of 0.88% potassium chloride were added
姜不辣and the mixture was shaken. The aqueous layer (upper)
was removed by aspiration and the washing procedure
was repeated. The extract was then dried by adding
五边形英语
anhydrous sodium sulphate, which was filtered again,
before the solvent was removed using a rotary evaporator.
普希金的代表作
The extract was then placed in a desiccator
overnight and weighed
Extraction by acid hydrolysis
difficult的反义词Homogenid tissue (10 g) was progressively added to
small amounts of a chloroform/methanol 2:1 (v/v) mixture
(up to 200 ml), with vigorous shaking, and then the
extraction was carried on for a further 2h, using an
electromagnetic stirrer. The mixture was filtered and the
filter was re-washed with fresh solvent and presd.
Fifty millilitres of 0.88% potassium chloride were added
and the mixture was shaken. The aqueous layer (upper)
was removed by aspiration and the washing procedure
was repeated. The extract was then dried by adding
anhydrous sodium sulphate, which was filtered again,
before the solvent was removed using a rotary evaporator.
The extract was then placed in a desiccator
overnight and weighed
Extraction by ASE
