目录
缩略词表 (1)
英文摘要 (2)心静的成语
中文摘要 (7)
论文正文张力刺激对髓核细胞CILP表达的调控及CILP对基质合成表型的影响 .. 11第一章前言 (11)
第二章 CILP在不同退变程度髓核组织中的差异性表达 (12)
2.1 在轻度退变组和严重退变组中的差异表达 (12)
2.2 小结 (16)
第三章张力刺激对CILP以及基质合成表型的调控效应 (17)
一举一动的意思3.1 CILP的张力调控及机制 (17)
3.2 小结 (26)
第四章 CILP通过抑制髓核细胞的基质合成表性促进椎间盘退变进程 (27)
4.1 CILP对基质合成表型的抑制效应 (27)
4.2 小结 (34)
全文结论 (35)
参考文献 (36)
文献综述软骨层间蛋白 (45)
参考文献 (48)
读硕士学位期间发表论文情况 (53)
致谢 (54)
缩略词表
英文缩写英文全称中文全称
LBP NP Low back pain
Nucleus pulposus
腰背痛
髓核
CILP Cartilage intermediate layer protein 软骨层间蛋白ECM Extracellular
matrix 细胞外基质
Col II Collagen II II型胶原蛋白Agg Aggrecan 蛋白多糖
牛膝功效与作用
CTS Cyclic tensional stimuli 循环性张力刺激
IDD IVD Intervertebral disc degeneration
Intervertebraal disc
椎间盘退变
椎间盘
Smad Drosophila
mothers
against decapentaplegic
protein
GAPDH Glyceraldehyde
phosphate dehydrogena 磷酸甘油醛脱氢酶SDS Sodium dodecyl sulfonate 十二烷基磺酸钠TBST Tris buffered saline tween 洗膜缓冲液
DMEM Dulbecco’s modified eagle’s medium
MAPK Mitogen activated protein kina 丝裂原活化蛋白激酶OA Osteoarthritis 骨关节炎
IOD Integrated optical density 累计光密度值
ERK Extracellular signal-regulated kina 细胞外信号调节激酶
BMP-2 IGF-1 SNP MMP ADAMT
AGC COMT VDR bone morphogenetic protein-2
Insulinlike growth factor-1
Single nucleotide polymorphism
matrix metalloproteina
A disintegrin and metalloprotea with
一二三四五六七
thrombospondin I type motif
Aggrecan 1
Catechol-O-methyltransfera
VitD receptor
骨形态发生蛋白-2
类胰岛素生长因子-1
单脱氧核苷酸突变
基质金属蛋白酶
含I型血小板结合蛋白基序的
爬叉
解聚蛋白样金属蛋白酶
蛋白聚糖1
福州北峰
儿茶酚氧位甲基转移酶
维他命D受体
CILP is regulated by tension stimulation and affects the
synthesis of matrix phenotype
翡翠鉴定
Abstract
Background
Low back pain(LBP) is one of the main disorders that has drew attention of scientists for its threat to people’s health. 70-80% of individuals in the world have experienced LBP at some time in their lives, of which a large percentage underwent surgeries, casting a heavy economic burden on families and society. Generally, Intervertebral disc degeneration (IDD) is regarded as the main cau contributing to the initiation and progress of LBP. With respect to the complicated pathology in IDD associated with susceptible genes, excessive mechanical loading, incread cellular nescence, nutrition failure and so on, more studies are required to identify critical factors that play important roles in IDD and cast a detailed knowledge of the mechanism in IDD.
Cartilage intermediate layer protein (CILP) is restrictively distributed in articular cartilage and intervertebral discs (IVDs). Previous studies have shown an incread expression of CILP in aging discs and revealed the correlation of a functional single nucleotide polymorphism (SNP) in 1184 allele of CILP gene with the incidence of disc dias, which indicates the possible involvement of CILP in IDD. Further, it has been validated that the polymorphism in 1184 allele increas the affinity of CILP for transforming growth factor-beta (TGF-β), handicaping the interaction of TGF-β with its receptor, suggesting that CILP may be involved in IDD by interfering in the interaction of some growt
笑歌词h factors with resident cells. Similarly, CILP is also able to decrea the pro-anabolism effect of IGF-1 on chondrocyte and indirectly promote PPi supersaturation in bovine aging and osteoarthritis cartilage. Moreover, in transgenic mice over-expressing CILP, a low signal was detected by MRI at early stage, indicating an early degeneration initiated in IVDs, which suggests the ability of CILP to launch the initiation of degeneration. In summary, the irregularly expresd CILP in IDD may not just be a landmark for degeneration, but also contribute to the progress as an accelerator.
