Determination of Catecholamines in Plasma by HPLC

更新时间:2023-07-21 00:35:31 阅读: 评论:0

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Determination of Catecholamines in Plasma by HPLC and Amperometric Detection. Comparison with a Radioenzymatic Method 期刊名称: J Clin Chem Clin Biochem
作者: W. Bauersfeld,D. Ratge,E. Knoll,H. Wisr
年份: 1986年
蚕茧的作用期号: 第3期
山药的功效和作用关键词: Linkage;helicity;magnetohydrodynamics;solar magnetism
申请困难补助理由摘要:The determination of norepinephrine and epinephrine in plasma by HPLC with amperometric detection was modified, giving detection limits of 25 ng/l for norepinephrine and epinephrine, respectively, using 1 ml plasma. In order to achieve this nsitivity, it was necessary to minimize the background noi by modification of instrumentation and specimen handling. Particularly important was the extra purification of the reagents, the application of micro-bore HPLC, the enzymatic cleavage of uric acid and temperature control of the amperometric cell and the amplifier. Comparison of the pres
ent method with the radioenzymatic determination of catecholamines resulted in coefficients of correlation of r = 0.924 and 0.919 for norepinephrine and epinephrine, resp. (n = 38). The concentrations of the 38 different samples ud for the
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